From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2451 [new locus tag: SACOL_RS12865 ]
  • pan locus tag?: SAUPAN005998000
  • symbol: SACOL2451
  • pan gene symbol?: opuCC
  • synonym:
  • product: amino acid ABC transporter amino acid-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2451 [new locus tag: SACOL_RS12865 ]
  • symbol: SACOL2451
  • product: amino acid ABC transporter amino acid-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2510752..2511693
  • length: 942
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGAAAATTAAATATATACTTGTCGTGTTTGTCTTATCGCTTACCGTATTATCTGGA
    TGTAGTTTGCCCGGACTAGGTAGTAAGAGCACGAAAAATGATGTCAAAATTACAGCATTA
    TCAACAAGCGAATCGCAAATTATTTCACATATGTTACGGTTGTTAATAGAGCATGATACA
    CACGGTAAGATAAAGCCAACATTAGTAAATAATTTAGGGTCAAGTACGATTCAACATAAT
    GCCTTAATTAATGGGGATGCTAATATATCAGGTGTTAGATATAATGGCACAGATTTAACG
    GGAGCTTTGAAGGAAGCACCAATTAAAAATCCTAAGAAAGCAATGATAGCAACACAACAA
    GGATTTAAAAAGAAATTTGATCAAACGTTTTTTGATTCGTATGGTTTTGCGAATACGTAT
    GCATTCATGGTAACGAAGGAAACCGCTAAAAAATATCATTTAGAGACAGTTTCAGATTTA
    GCAAAGCATAGTAAAGATTTACGTTTAGGTATGGATAGTTCATGGATGAATCGTAAAGGC
    GATGGCTATGAAGGATTTAAAAAAGAGTATGGTTTTGACTTTGGTACAGTGAGACCAATG
    CAAATAGGTCTAGTCTACGACGCATTAAACTCAGAGAAGTTAGACGTTGCATTAGGTTAT
    TCTACAGATGGTCGAATTGCGGCGTATGATTTGAAAGTACTTAAAGATGATAAACAATTT
    TTCCCACCTTATGCTGCGAGTGCTGTTGCAACAAATGAATTATTACGGCAACACCCAGAA
    CTTAAAACGACGATTAATAAGTTGACAGGAAAGATTTCGACTTCAGAGATGCAACGCTTG
    AATTATGAAGCGGATGGTAAAGGTAAAGAACCTGCTGTCGTCGCAGAAGAATTTTTGAAA
    AAACACCACTATTTTGATAAACAGAAAGGTGGTCATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2451 [new locus tag: SACOL_RS12865 ]
  • symbol: SACOL2451
  • description: amino acid ABC transporter amino acid-binding protein
  • length: 313
  • theoretical pI: 9.89386
  • theoretical MW: 34812.7
  • GRAVY: -0.4377

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 14.7)
  • TheSEED  :
    • Osmotically activated L-carnitine/choline ABC transporter, substrate-binding protein OpuCC
    Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Osmotically activated L-carnitine/choline ABC transporter, substrate-binding protein OpuCC
  • PFAM:
    PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 180)
    and 2 more
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.9)
    no clan defined Nrap_D2; Nrap protein PAP/OAS-like domain (PF17403; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: TVLSGCS
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.001304
    • TAT(Tat/SPI): 0.000196
    • LIPO(Sec/SPII): 0.998173
    • Cleavage Site: CS pos: 20-21. LSG-CS. Pr: 0.9989
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSESQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGALKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDLAKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVALGYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSEMQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2451&oldid=82099"