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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2555 [new locus tag: SACOL_RS13380 ]
  • pan locus tag?: SAUPAN006199000
  • symbol: SACOL2555
  • pan gene symbol?: cidR
  • synonym:
  • product: LysR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2555 [new locus tag: SACOL_RS13380 ]
  • symbol: SACOL2555
  • product: LysR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2614414..2615298
  • length: 885
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTTAGTGGATATCAAACATATGAAATATTTTATTGAAGTCGTTAAGCAAGGAGGCATG
    ACTAATGCTTCCAAATCATTATATATTGCACAACCTACAATTAGTAAAGCAATTAAAGAT
    ATTGAAAATGAAATGGGCACGCCCTTATTTGATAGAAGTAAAAGACATTTAATTCTTACC
    GATGCAGGTCAAATTTTTTATGAGAAAAGTAAAGAAATTGTTGCACTGTATGATTATTTA
    CCATCTGAAATGGAACGCTTGAATGGACTGGAAACAGGACATATAAACATGGGCATGTCG
    GCAGTCATGAATATGAAGATTCTTATCAATATTCTTGGTGCATTCCATCAACAATATCCA
    AATGTTACATATAATTTGATAGAAAATGGCGGTAAAACAATTGAACAGCAAATTATCAAT
    GATGAAGTAGATATAGGCGTGACCACTTTGCCAGTCGATCATCATATTTTCGATTATACT
    ACCCTAGATAAGGAAGATTTGCGACTTATCGTGAGCAGAGAGCATCGACTCGCAAAATAT
    GAAACTGTTAAACTCGAAGATTTAGCAGGTGAAGACTTCATTTTATTTAATAAAGACTTT
    TACTTGAATGATAAAATTATTGAAAATGCTAAAAACGTTGGCTTTGTTCCGAATACTGTA
    GCGCAAATTTCACAATGGCATGTTATAGAAGATTTAGTTACGAATGAATTAGGTATTAGT
    ATTTTACCAACATCAATTTCAGAGCAACTAAATGGAGATGTGAAGCTGCTACGCATTGAA
    GATGCTCATGTACATTGGGAATTAGGTGTTGTTTGGAAGAAGGATAAACAATTAAGTCAT
    GCCACAACGAAATGGATAGAATTTTTGAAAGACCGTTTAGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    885

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2555 [new locus tag: SACOL_RS13380 ]
  • symbol: SACOL2555
  • description: LysR family transcriptional regulator
  • length: 294
  • theoretical pI: 5.38489
  • theoretical MW: 33683.5
  • GRAVY: -0.207483

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 131.8)
    and 9 more
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 89.7)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 89.7)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 57.2)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 34.6)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 15)
    Signal transduction Regulatory functions Other ModE molybdate transport repressor domain (TIGR00637; HMM-score: 13)
  • TheSEED  :
    • Transcriptional regulator CidR, LysR family
    Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  LysR family regulatory protein CidR
  • PFAM:
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 143.7)
    and 2 more
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 67.8)
    no clan defined ERp29; Endoplasmic reticulum protein ERp29, C-terminal domain (PF07749; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008165
    • TAT(Tat/SPI): 0.001162
    • LIPO(Sec/SPII): 0.001815
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLVDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMGTPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMNMKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTLDKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTVAQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQLSHATTKWIEFLKDRLG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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