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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2480 [new locus tag: SAUSA300_RS13760 ]
  • pan locus tag?: SAUPAN006199000
  • symbol: SAUSA300_2480
  • pan gene symbol?: cidR
  • synonym:
  • product: LysR family transcriptional regulator

cidR: transcriptional activator of cellular suicide operon

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2480 [new locus tag: SAUSA300_RS13760 ]
  • symbol: SAUSA300_2480
  • product: LysR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2677719..2678606
  • length: 888
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGATGTTAGTGGATATCAAACATATGAAATATTTTATTGAAGTCGTTAAGCAAGGAGGC
    ATGACTAATGCTTCCAAATCATTATATATTGCACAACCTACAATTAGTAAAGCAATTAAA
    GATATTGAAAATGAAATGGGCACGCCCTTATTTGATAGAAGTAAAAGACATTTAATTCTT
    ACCGATGCAGGTCAAATTTTTTATGAGAAAAGTAAAGAAATTGTTGCACTGTATGATTAT
    TTACCATCTGAAATGGAACGCTTGAATGGACTGGAAACAGGACATATAAACATGGGCATG
    TCGGCAGTCATGAATATGAAGATTCTTATCAATATTCTTGGTGCATTCCATCAACAATAT
    CCAAATGTTACATATAATTTGATAGAAAATGGCGGTAAAACAATTGAACAGCAAATTATC
    AATGATGAAGTAGATATAGGCGTGACCACTTTGCCAGTCGATCATCATATTTTCGATTAT
    ACTACCCTAGATAAGGAAGATTTGCGACTTATCGTGAGCAGAGAGCATCGACTCGCAAAA
    TATGAAACTGTTAAACTCGAAGATTTAGCAGGTGAAGACTTCATTTTATTTAATAAAGAC
    TTTTACTTGAATGATAAAATTATTGAAAATGCTAAAAACGTTGGCTTTGTTCCGAATACT
    GTAGCGCAAATTTCACAATGGCATGTTATAGAAGATTTAGTTACGAATGAATTAGGTATT
    AGTATTTTACCAACATCAATTTCAGAGCAACTAAATGGAGATGTGAAGCTGCTACGCATT
    GAAGATGCTCATGTACATTGGGAATTAGGTGTTGTTTGGAAGAAGGATAAACAATTAAGT
    CATGCCACAACGAAATGGATAGAATTTTTGAAAGACCGTTTAGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    888

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2480 [new locus tag: SAUSA300_RS13760 ]
  • symbol: SAUSA300_2480
  • description: LysR family transcriptional regulator
  • length: 295
  • theoretical pI: 5.38489
  • theoretical MW: 33814.7
  • GRAVY: -0.200339

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 131.8)
    and 9 more
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 89.7)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 89.7)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 57.2)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 34.6)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 15)
    Signal transduction Regulatory functions Other ModE molybdate transport repressor domain (TIGR00637; HMM-score: 13)
  • TheSEED  :
    • Transcriptional regulator CidR, LysR family
    Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  LysR family regulatory protein CidR
  • PFAM:
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 143.7)
    and 2 more
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 67.8)
    no clan defined ERp29; Endoplasmic reticulum protein ERp29, C-terminal domain (PF07749; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007614
    • TAT(Tat/SPI): 0.001102
    • LIPO(Sec/SPII): 0.000968
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMLVDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMGTPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMNMKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTLDKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTVAQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQLSHATTKWIEFLKDRLG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Marat R Sadykov, Ian H Windham, Todd J Widhelm, Vijaya Kumar Yajjala, Sean M Watson, Jennifer L Endres, Arissa I Bavari, Vinai C Thomas, Jeffrey L Bose, Kenneth W Bayles
    CidR and CcpA Synergistically Regulate Staphylococcus aureus cidABC Expression.
    J Bacteriol: 2019, 201(23);
    [PubMed:31501288] [WorldCat.org] [DOI] (I e)    Soo-Jin Yang, Paul M Dunman, Steven J Projan, Kenneth W Bayles
    Characterization of the Staphylococcus aureus CidR regulon: elucidation of a novel role for acetoin metabolism in cell death and lysis.
    Mol Microbiol: 2006, 60(2);458-68
    [PubMed:16573694] [WorldCat.org] [DOI] (P p)    Soo-Jin Yang, Kelly C Rice, Raquel J Brown, Toni G Patton, Linda E Liou, Yong Ho Park, Kenneth W Bayles
    A LysR-type regulator, CidR, is required for induction of the Staphylococcus aureus cidABC operon.
    J Bacteriol: 2005, 187(17);5893-900
    [PubMed:16109930] [WorldCat.org] [DOI] (P p)
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