PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2480 [new locus tag: SAUSA300_RS13760 ]
• pan locus tag^?: SAUPAN006199000
• symbol: SAUSA300_2480
• pan gene symbol^?: cidR
• synonym:
• product: LysR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2480 [new locus tag: SAUSA300_RS13760 ]
• symbol: SAUSA300_2480
• product: LysR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 2677719..2678606
• length: 888
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914891 NCBI
• RefSeq: YP_495114 NCBI
• BioCyc: see SAUSA300_RS13760
• MicrobesOnline: 1293995 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATGTTAGTGGATATCAAACATATGAAATATTTTATTGAAGTCGTTAAGCAAGGAGGC
  ATGACTAATGCTTCCAAATCATTATATATTGCACAACCTACAATTAGTAAAGCAATTAAA
  GATATTGAAAATGAAATGGGCACGCCCTTATTTGATAGAAGTAAAAGACATTTAATTCTT
  ACCGATGCAGGTCAAATTTTTTATGAGAAAAGTAAAGAAATTGTTGCACTGTATGATTAT
  TTACCATCTGAAATGGAACGCTTGAATGGACTGGAAACAGGACATATAAACATGGGCATG
  TCGGCAGTCATGAATATGAAGATTCTTATCAATATTCTTGGTGCATTCCATCAACAATAT
  CCAAATGTTACATATAATTTGATAGAAAATGGCGGTAAAACAATTGAACAGCAAATTATC
  AATGATGAAGTAGATATAGGCGTGACCACTTTGCCAGTCGATCATCATATTTTCGATTAT
  ACTACCCTAGATAAGGAAGATTTGCGACTTATCGTGAGCAGAGAGCATCGACTCGCAAAA
  TATGAAACTGTTAAACTCGAAGATTTAGCAGGTGAAGACTTCATTTTATTTAATAAAGAC
  TTTTACTTGAATGATAAAATTATTGAAAATGCTAAAAACGTTGGCTTTGTTCCGAATACT
  GTAGCGCAAATTTCACAATGGCATGTTATAGAAGATTTAGTTACGAATGAATTAGGTATT
  AGTATTTTACCAACATCAATTTCAGAGCAACTAAATGGAGATGTGAAGCTGCTACGCATT
  GAAGATGCTCATGTACATTGGGAATTAGGTGTTGTTTGGAAGAAGGATAAACAATTAAGT
  CATGCCACAACGAAATGGATAGAATTTTTGAAAGACCGTTTAGGCTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2480 [new locus tag: SAUSA300_RS13760 ]
• symbol: SAUSA300_2480
• description: LysR family transcriptional regulator
• length: 295
• theoretical pI: 5.38489
• theoretical MW: 33814.7
• GRAVY: -0.200339

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 131.8)
  and 9 more
  Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 89.7)
  Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 89.7)
  putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 57.2)
  Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 43.6)
  Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 34.6)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 15)
  Signal transduction Regulatory functions Other ModE molybdate transport repressor domain (TIGR00637; HMM-score: 13)
• TheSEED  :
  • Transcriptional regulator CidR, LysR family
  Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  LysR family regulatory protein CidR
• PFAM:
  PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 143.7)
  and 2 more
  HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 67.8)
  no clan defined ERp29; Endoplasmic reticulum protein ERp29, C-terminal domain (PF07749; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007614
  • TAT(Tat/SPI): 0.001102
  • LIPO(Sec/SPII): 0.000968
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161994 NCBI
• RefSeq: YP_495114 NCBI
• UniProt: A0A0H2XI75 UniProt

⊟Protein sequence

• MMLVDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMGTPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMNMKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTLDKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTVAQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQLSHATTKWIEFLKDRLG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications