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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2562 [new locus tag: SACOL_RS13420 ]
- pan locus tag?: SAUPAN006209000
- symbol: ogt
- pan gene symbol?: —
- synonym:
- product: methylated-DNA--protein-cysteine methyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2562 [new locus tag: SACOL_RS13420 ]
- symbol: ogt
- product: methylated-DNA--protein-cysteine methyltransferase
- replicon: chromosome
- strand: -
- coordinates: 2620960..2621481
- length: 522
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238673 NCBI
- RefSeq: YP_187354 NCBI
- BioCyc: see SACOL_RS13420
- MicrobesOnline: 914033 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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481ATGGAGTATAAGAGTTACTATGATTCGCCTGTAGGACGATTGGAACTTTTGAGTGATGGG
GTAAGTCTGACCGCTGTTTTGTTTGAAAATCAACAAGGTGATGGTACTAGGGAAGAAAAT
ACGTCTTTAGCGATATTTAGAGAGGCGACACAGTGGTTAGACGCGTATTTTAAAGGTGAC
AATCCTGAAATCACAATACCTTTAAAGCCAACAGGTAGTCATTTTCAACAGTGTGTTTGG
AATGAATTAAGACAGGTTCCTTATGGGACTCTAACAACGTATGGTGCTATTGCCAAAAAA
GTAGGCAAGCTGCTTAACAAGCCAAAAATGTCAGCCCAAGCGGTTGGAGGTGCAGTCGGA
AGTAATCCATTATCTATTATCGTGCCATGTCATCGTGTTGTCGGTAAAACGGGTAGCTTA
ACAGGGTTCGGTGGAACGATTAATAATAAAATTAAGCTGTTAGAGTTGGAGAATATCGAT
ATGTCAAAGCTTTATGTACCAAAACATAGCACGAAGCCGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2562 [new locus tag: SACOL_RS13420 ]
- symbol: Ogt
- description: methylated-DNA--protein-cysteine methyltransferase
- length: 173
- theoretical pI: 9.01352
- theoretical MW: 18945.6
- GRAVY: -0.29422
⊟Function[edit | edit source]
- reaction: EC 2.1.1.63? ExPASyMethylated-DNA--[protein]-cysteine S-methyltransferase DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair methylated-DNA--[protein]-cysteine S-methyltransferase (TIGR00589; EC 2.1.1.63; HMM-score: 92.5)
- TheSEED :
- Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)
- PFAM: no clan defined DNA_binding_1; 6-O-methylguanine DNA methyltransferase, DNA binding domain (PF01035; HMM-score: 103.1)and 1 moreMethyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain (PF02870; HMM-score: 30.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.006297
- TAT(Tat/SPI): 0.000595
- LIPO(Sec/SPII): 0.001318
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLAIFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVGKLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENIDMSKLYVPKHSTKP
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: 29.96 h [4]
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)