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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2485 [new locus tag: SAUSA300_RS13800 ]
  • pan locus tag?: SAUPAN006209000
  • symbol: SAUSA300_2485
  • pan gene symbol?: —
  • synonym:
  • product: methylated DNA-protein cysteine methyltransferase

adaB (ogt): methylated-DNA:protein-cysteine methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2485 [new locus tag: SAUSA300_RS13800 ]
  • symbol: SAUSA300_2485
  • product: methylated DNA-protein cysteine methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2684265..2684786
  • length: 522
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGAGTATAAGAGTTACTATGATTCGCCTGTAGGACGATTGGAACTTTTGAGTGATGGG
    GTAAGTCTGACCGCTGTTTTGTTTGAAAATCAACAAGGTGATGGTACTAGGGAAGAAAAT
    ACGTCTTTAGCGATATTTAGAGAGGCGACACAGTGGTTAGACGCGTATTTTAAAGGTGAC
    AATCCTGAAATCACAATACCTTTAAAGCCAACAGGTAGTCATTTTCAACAGTGTGTTTGG
    AATGAATTAAGACAGGTTCCTTATGGGACTCTAACAACGTATGGTGCTATTGCCAAAAAA
    GTAGGCAAGCTGCTTAACAAGCCAAAAATGTCAGCCCAAGCGGTTGGAGGTGCAGTCGGA
    AGTAATCCATTATCTATTATCGTGCCATGTCATCGTGTTGTCGGTAAAACGGGTAGCTTA
    ACAGGGTTCGGTGGAACGATTAATAATAAAATTAAGCTGTTAGAGTTGGAGAATATCGAT
    ATGTCAAAGCTTTATGTACCAAAACATAGCACGAAGCCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    522

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2485 [new locus tag: SAUSA300_RS13800 ]
  • symbol: SAUSA300_2485
  • description: methylated DNA-protein cysteine methyltransferase
  • length: 173
  • theoretical pI: 9.01352
  • theoretical MW: 18945.6
  • GRAVY: -0.29422

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.63?  ExPASy
    Methylated-DNA--[protein]-cysteine S-methyltransferase DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair methylated-DNA--[protein]-cysteine S-methyltransferase (TIGR00589; EC 2.1.1.63; HMM-score: 92.5)
  • TheSEED  :
    • Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)
    DNA Metabolism DNA repair DNA repair, bacterial  Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)
  • PFAM:
    no clan defined DNA_binding_1; 6-O-methylguanine DNA methyltransferase, DNA binding domain (PF01035; HMM-score: 103.1)
    and 1 more
    Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain (PF02870; HMM-score: 30.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006297
    • TAT(Tat/SPI): 0.000595
    • LIPO(Sec/SPII): 0.001318
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLAIFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVGKLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENIDMSKLYVPKHSTKP

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

Technically, AdaB (OGT) is not an enzyme because it is consumed in the reaction. It catalyzes the transfer of a methyl group from O6-methylguanine or O4-methylthymidine within damaged DNA onto an active site cysteine, repairing the DNA but rendering the AdaB "enzyme" nonfunctional.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Kam Pou Ha, Andrew M Edwards
    DNA Repair in Staphylococcus aureus.
    Microbiol Mol Biol Rev: 2021, 85(4);e0009121
    [PubMed:34523959] [WorldCat.org] [DOI] (I p)
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