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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2566 [new locus tag: SACOL_RS13445 ]
  • pan locus tag?: SAUPAN006214000
  • symbol: SACOL2566
  • pan gene symbol?: farE
  • synonym:
  • product: MmpL efflux pump

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2566 [new locus tag: SACOL_RS13445 ]
  • symbol: SACOL2566
  • product: MmpL efflux pump
  • replicon: chromosome
  • strand: -
  • coordinates: 2626696..2629185
  • length: 2490
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    TTGGCAAAATTTTTATATAAGATGGGGACATTCATCGCAAAACATAAATGGTCCGCGGTC
    ATTGCATGGATAGTGATTGTAGCTGCAATATTGATACCACTTGCTACAAATGCACCGAAA
    TTTGACAATGATATTAAAATGACAGGATTAGAGTCGTTAGATACGAATAAAAAGATTGAA
    AAACATTTTAATCAAGATAGTGAAAAGGCACAAATACGTGTTGTCTTTAAGACGACTAAA
    GATGATGGTATTGTGCAACCGAACATTACTAAAGATATTAAGAAAACGTTAGATGATATT
    AAAAAAGATGACAAGCACATTGATAAAATATCAGATCCTTATGAAAATAAGCAAATTAGT
    AAAGATAAAACAACTGCATTTGCAGATATCACATATGACGTTAGTCAAACATCTTTAAAA
    GATGGCTCTAGAGATAATGTTAAAAGTCATTTGAAAGACTTACGTGACAATCATAATGTA
    CAAACAGAGTTAACTGGTACAGGAATGACATCTACAGAAGTAGGCGGTAATTCTGAACTA
    GTTGGTATTATTGTTGCATTTGTTGTACTGTTAATCACATTCGGTTCAGTCATTGCTGCA
    GGATTACCAATTATAAGTGCATTGATTGGTCTAGCCTCTGGTGTAGGTATTATCAGTTTG
    TTAACTTATGCATTCGATATACCAAATGTCACACTGACATTAGCCGTAATGATTGGACTA
    GCAGTTGGTATCGATTATGCATTATTTATTTTATTCAGATATCGACAAGTTATGAAAACT
    GAAACAGATTACATTAAAGGCATTGGTCTTGCAATAGGAACAGCAGGTAGTGCAGTTGTT
    TTTGCTGGTGTAACAGTTGTAATTGCAGTATGTGGTTTATCATTAGTAGGCATTGACTTC
    TTAGCGGTAATGGGATTTGCTTCGGCAATTAGTGTTGTTTTTGCAGTACTTAGCGCTTTA
    ACTTTATTACCTGCATTAATAAGCATTTTCCATAAACGCATTAAAGTGAATAAATTACAG
    TCTAAATTCAAAAAAGATATTGATACGCCATGGTCTAAATTTATTACAGGAAATGCACTT
    GCGGCAGTATTACTAGGTTTAATCATATTAGTAGCAGCTGCAATACCAGTTAGTCATATG
    CGACTTGGTATTCCAGATGACGGTGTTAAACCAGCAGATTCTACACAGAAAAAAGCTTAC
    GATATTATCTCGGATAAATTTGGTGAAGGATTTAATGGCCAAATACCGATGTTGATAAAT
    GTGAAAGATAAAAAGGATGATCCACAAGGATTACAACAAGATTTACAGTCTGTATATAAA
    GATATTAAAGATAAGAAAAATGTAGATATCGTTACGCCACCACAAATGAGTAAAGATAAT
    GATTACGCTTTGATGGTCGTGATACCTAAGCAAGGGCCTAATGCAGAGAGTACAAATGAT
    TTAGTGCATGATTTACGAGATTATCATAAAGATGCACAAGATAAATATGGGTTCAAAACG
    GAGATTTCGGGACAAAGTGTCATTAATATTGATATGTCTAAAAAGTTAAATGAGGCGATA
    CCTTTATTTGCCTCAGTCATTGTTGTATTAGCATTTTTCTTATTAATGATTGTCTTCCGT
    TCAATTCTAATACCATTAAAAGCAGTTTTAGGATTCGTCTTGTCATTAATGGCAACATTA
    GGATTTACAACATTAGTAATGCAAGATGGTTTTATGAAAGGTCTGTTTGGTATTGAAACG
    ACAGGACCAATGCTAGCATTTTTACCGGTTATTACTATAGGTATCTTATTTGGATTAGCG
    ATGGATTATGAAGTCTTCCTTATGTCGCGAATTCATGAAGAATATAGTAAAACAGGGGAT
    AATGATTATTCTATCAAAGTAGGATTGAAAGAAAGCGGTCCTGTTATTGTAGCAGCTGCA
    TTAATTATGTTTAGTGTCTTCTTTGCGTTTGTATTCCAAGAAGATGTCATGATTAAATCA
    ATGGGTATGGCATTAGCGTTTGGTGTACTCTTTGATGCCTTCGTAGTCAGAATGATGTTA
    ATTCCTGCATTAACGAAATTGTTTGGAAAAGGTTCGTGGTATTTACCAGCATGGTTGAAT
    CGCATTATTCCACGAGTTGATATTGAAGGACATGCACTTGAAAAATATAAAACGGTTGAA
    TCACAGGAATCTGAAGCTAAAGATAGTAAGGAAACATACGACACAACCTTTAAAGTATAT
    CCACAAGGTGCAACAAATGTATCGAAACATCAAGATGCACATGGGCAAGATGATGCACAT
    TCAATAGTACTAGATGATAAAACGATGGCATTGTACCAAGAAGTGAAACAGCAATCAGCA
    AGTTCATTGTTCTTATATGATGCTTTAATAGATTATCAAAACAAGCATCAACTAAATTCA
    AAACAACAAGCAACGAATATAGAACAGTTGAATAAAAATATTGAAAAGCTCAATCAATTG
    TTAGAAAAAAATCTACGAAATAAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2490

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2566 [new locus tag: SACOL_RS13445 ]
  • symbol: SACOL2566
  • description: MmpL efflux pump
  • length: 829
  • theoretical pI: 7.28458
  • theoretical MW: 91290.8
  • GRAVY: 0.158866

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 224.1)
    and 5 more
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 123.1)
    hopanoid biosynthesis associated RND transporter like protein HpnN (TIGR03480; HMM-score: 59.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 8.9)
    transmembrane receptor Patched (TIGR00918; HMM-score: 5.5)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecD (TIGR01129; HMM-score: 4.2)
  • TheSEED  :
    • Putative antibiotic transport-associated protein
    CBSS-196620.1.peg.2477  Putative antibiotic transport-associated protein
  • PFAM:
    RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 357.6)
    and 2 more
    no clan defined PAP_fibrillin; PAP_fibrillin (PF04755; HMM-score: 12.1)
    Fez1; Fez1 (PF06818; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 38 aa
    • SP(Sec/SPI): 0.544085
    • TAT(Tat/SPI): 0.004763
    • LIPO(Sec/SPII): 0.034836
    • Cleavage Site: CS pos: 38-39. TNA-PK. Pr: 0.1992
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDNDIKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKKDDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNVQTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGIISLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAGSAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRIKVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPADSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDIVTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVINIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLVMQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYSIKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIPALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVYPQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQLNSKQQATNIEQLNKNIEKLNQLLEKNLRNKS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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