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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
- pan locus tag?: SAUPAN006214000
- symbol: SAUSA300_2489
- pan gene symbol?: farE
- synonym:
- product: antibiotic transport-associated protein-like protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
- symbol: SAUSA300_2489
- product: antibiotic transport-associated protein-like protein
- replicon: chromosome
- strand: -
- coordinates: 2690001..2692469
- length: 2469
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913822 NCBI
- RefSeq: YP_495123 NCBI
- BioCyc: see SAUSA300_RS13825
- MicrobesOnline: 1294004 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2461ATGGGGACATTCATCGCAAAACATAAATGGTCCGCGGTCATTGCATGGATAGTGATTGTA
GCTGCAATATTGATACCACTTGCTACAAATGCACCGAAATTTGACAATGATATTAAAATG
ACAGGATTAGAGTCGTTAGATACGAATAAAAAGATTGAAAAACATTTTAATCAAGATAGT
GAAAAGGCACAAATACGTGTTGTCTTTAAGACGACTAAAGATGATGGTATTGTGCAACCG
AACATTACTAAAGATATTAAGAAAACGTTAGATGATATTAAAAAAGATGACAAGCACATT
GATAAAATATCAGATCCTTATGAAAATAAGCAAATTAGTAAAGATAAAACAACTGCATTT
GCAGATATCACATATGACGTTAGTCAAACATCTTTAAAAGATGGCTCTAGAGATAATGTT
AAAAGTCATTTGAAAGACTTACGTGACAATCATAATGTACAAACAGAGTTAACTGGTACA
GGAATGACATCTACAGAAGTAGGCGGTAATTCTGAACTAGTTGGTATTATTGTTGCATTT
GTTGTACTGTTAATCACATTCGGTTCAGTCATTGCTGCAGGATTACCAATTATAAGTGCA
TTGATTGGTCTAGCCTCTGGTGTAGGTATTATCAGTTTGTTAACTTATGCATTCGATATA
CCAAATGTCACACTGACATTAGCCGTAATGATTGGACTAGCAGTTGGTATCGATTATGCA
TTATTTATTTTATTCAGATATCGACAAGTTATGAAAACTGAAACAGATTACATTAAAGGC
ATTGGTCTTGCAATAGGAACAGCAGGTAGTGCAGTTGTTTTTGCTGGTGTAACAGTTGTA
ATTGCAGTATGTGGTTTATCATTAGTAGGCATTGACTTCTTAGCGGTAATGGGATTTGCT
TCGGCAATTAGTGTTGTTTTTGCAGTACTTAGCGCTTTAACTTTATTACCTGCATTAATA
AGCATTTTCCATAAACGCATTAAAGTGAATAAATTACAGTCTAAATTCAAAAAAGATATT
GATACGCCATGGTCTAAATTTATTACAGGAAATGCACTTGCGGCAGTATTACTAGGTTTA
ATCATATTAGTAGCAGCTGCAATACCAGTTAGTCATATGCGACTTGGTATTCCAGATGAC
GGTGTTAAACCAGCAGATTCTACACAGAAAAAAGCTTACGATATTATCTCGGATAAATTT
GGTGAAGGATTTAATGGCCAAATACCGATGTTGATAAATGTGAAAGATAAAAAGGATGAT
CCACAAGGATTACAACAAGATTTACAGTCTGTATATAAAGATATTAAAGATAAGAAAAAT
GTAGATATCGTTACGCCACCACAAATGAGTAAAGATAATGATTACGCTTTGATGGTCGTG
ATACCTAAGCAAGGGCCTAATGCAGAGAGTACAAATGATTTAGTGCATGATTTACGAGAT
TATCATAAAGATGCACAAGATAAATATGGGTTCAAAACGGAGATTTCGGGACAAAGTGTC
ATTAATATTGATATGTCTAAAAAGTTAAATGAGGCGATACCTTTATTTGCCTCAGTCATT
GTTGTATTAGCATTTTTCTTATTAATGATTGTCTTCCGTTCAATTCTAATACCATTAAAA
GCAGTTTTAGGATTCGTCTTGTCATTAATGGCAACATTAGGATTTACAACATTAGTAATG
CAAGATGGTTTTATGAAAGGTCTGTTTGGTATTGAAACGACAGGACCAATGCTAGCATTT
TTACCGGTTATTACTATAGGTATCTTATTTGGATTAGCGATGGATTATGAAGTCTTCCTT
ATGTCGCGAATTCATGAAGAATATAGTAAAACAGGGGATAATGATTATTCTATCAAAGTA
GGATTGAAAGAAAGCGGTCCTGTTATTGTAGCAGCTGCATTAATTATGTTTAGTGTCTTC
TTTGCGTTTGTATTCCAAGAAGATGTCATGATTAAATCAATGGGTATGGCATTAGCGTTT
GGTGTACTCTTTGATGCCTTCGTAGTCAGAATGATGTTAATTCCTGCATTAACGAAATTG
TTTGGAAAAGGTTCGTGGTATTTACCAGCATGGTTGAATCGCATTATTCCACGAGTTGAT
ATTGAAGGACATGCACTTGAAAAATATAAAACGGTTGAATCACAGGAATCTGAAGCTAAA
GATAGTAAGGAAACATACGACACAACCTTTAAAGTATATCCACAAGGTGCAACAAATGTA
TCGAAACATCAAGATGCACATGGGCAAGATGATGCACATTCAATAGTACTAGATGATAAA
ACGATGGCATTGTACCAAGAAGTGAAACAGCAATCAGCAAGTTCATTGTTCTTATATGAT
GCTTTAATAGATTATCAAAACAAGCATCAACTAAATTCAAAAAAACAAGCAACGAATATA
GAACAGTTGAATAAAAATATTGAAAAGCTCAATCAATTGTTAGAAAAAAATCTACGAAAT
AAATCATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
- symbol: SAUSA300_2489
- description: antibiotic transport-associated protein-like protein
- length: 822
- theoretical pI: 7.09563
- theoretical MW: 90408.7
- GRAVY: 0.158273
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 225.2)and 5 moreTransport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 121.3)hopanoid biosynthesis associated RND transporter like protein HpnN (TIGR03480; HMM-score: 60.9)Protein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 8.8)transmembrane receptor Patched (TIGR00918; HMM-score: 5)Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecD (TIGR01129; HMM-score: 4.3)
- TheSEED :
- Putative antibiotic transport-associated protein
- PFAM: RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 358.2)and 2 moreno clan defined PAP_fibrillin; PAP_fibrillin (PF04755; HMM-score: 11.8)Fez1; Fez1 (PF06818; HMM-score: 8.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 31 aa
- SP(Sec/SPI): 0.713169
- TAT(Tat/SPI): 0.006206
- LIPO(Sec/SPII): 0.045188
- Cleavage Site: CS pos: 31-32. TNA-PK. Pr: 0.2643
- predicted transmembrane helices (TMHMM): 12
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDNDIKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKKDDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNVQTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGIISLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAGSAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRIKVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPADSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDIVTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVINIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLVMQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYSIKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIPALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVYPQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQLNSKKQATNIEQLNKNIEKLNQLLEKNLRNKS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
regulated by farR (SAUSA300_2490)
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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