PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
• pan locus tag^?: SAUPAN006214000
• symbol: SAUSA300_2489
• pan gene symbol^?: farE
• synonym:
• product: antibiotic transport-associated protein-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
• symbol: SAUSA300_2489
• product: antibiotic transport-associated protein-like protein
• replicon: chromosome
• strand: -
• coordinates: 2690001..2692469
• length: 2469
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913822 NCBI
• RefSeq: YP_495123 NCBI
• BioCyc: see SAUSA300_RS13825
• MicrobesOnline: 1294004 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGGACATTCATCGCAAAACATAAATGGTCCGCGGTCATTGCATGGATAGTGATTGTA
  GCTGCAATATTGATACCACTTGCTACAAATGCACCGAAATTTGACAATGATATTAAAATG
  ACAGGATTAGAGTCGTTAGATACGAATAAAAAGATTGAAAAACATTTTAATCAAGATAGT
  GAAAAGGCACAAATACGTGTTGTCTTTAAGACGACTAAAGATGATGGTATTGTGCAACCG
  AACATTACTAAAGATATTAAGAAAACGTTAGATGATATTAAAAAAGATGACAAGCACATT
  GATAAAATATCAGATCCTTATGAAAATAAGCAAATTAGTAAAGATAAAACAACTGCATTT
  GCAGATATCACATATGACGTTAGTCAAACATCTTTAAAAGATGGCTCTAGAGATAATGTT
  AAAAGTCATTTGAAAGACTTACGTGACAATCATAATGTACAAACAGAGTTAACTGGTACA
  GGAATGACATCTACAGAAGTAGGCGGTAATTCTGAACTAGTTGGTATTATTGTTGCATTT
  GTTGTACTGTTAATCACATTCGGTTCAGTCATTGCTGCAGGATTACCAATTATAAGTGCA
  TTGATTGGTCTAGCCTCTGGTGTAGGTATTATCAGTTTGTTAACTTATGCATTCGATATA
  CCAAATGTCACACTGACATTAGCCGTAATGATTGGACTAGCAGTTGGTATCGATTATGCA
  TTATTTATTTTATTCAGATATCGACAAGTTATGAAAACTGAAACAGATTACATTAAAGGC
  ATTGGTCTTGCAATAGGAACAGCAGGTAGTGCAGTTGTTTTTGCTGGTGTAACAGTTGTA
  ATTGCAGTATGTGGTTTATCATTAGTAGGCATTGACTTCTTAGCGGTAATGGGATTTGCT
  TCGGCAATTAGTGTTGTTTTTGCAGTACTTAGCGCTTTAACTTTATTACCTGCATTAATA
  AGCATTTTCCATAAACGCATTAAAGTGAATAAATTACAGTCTAAATTCAAAAAAGATATT
  GATACGCCATGGTCTAAATTTATTACAGGAAATGCACTTGCGGCAGTATTACTAGGTTTA
  ATCATATTAGTAGCAGCTGCAATACCAGTTAGTCATATGCGACTTGGTATTCCAGATGAC
  GGTGTTAAACCAGCAGATTCTACACAGAAAAAAGCTTACGATATTATCTCGGATAAATTT
  GGTGAAGGATTTAATGGCCAAATACCGATGTTGATAAATGTGAAAGATAAAAAGGATGAT
  CCACAAGGATTACAACAAGATTTACAGTCTGTATATAAAGATATTAAAGATAAGAAAAAT
  GTAGATATCGTTACGCCACCACAAATGAGTAAAGATAATGATTACGCTTTGATGGTCGTG
  ATACCTAAGCAAGGGCCTAATGCAGAGAGTACAAATGATTTAGTGCATGATTTACGAGAT
  TATCATAAAGATGCACAAGATAAATATGGGTTCAAAACGGAGATTTCGGGACAAAGTGTC
  ATTAATATTGATATGTCTAAAAAGTTAAATGAGGCGATACCTTTATTTGCCTCAGTCATT
  GTTGTATTAGCATTTTTCTTATTAATGATTGTCTTCCGTTCAATTCTAATACCATTAAAA
  GCAGTTTTAGGATTCGTCTTGTCATTAATGGCAACATTAGGATTTACAACATTAGTAATG
  CAAGATGGTTTTATGAAAGGTCTGTTTGGTATTGAAACGACAGGACCAATGCTAGCATTT
  TTACCGGTTATTACTATAGGTATCTTATTTGGATTAGCGATGGATTATGAAGTCTTCCTT
  ATGTCGCGAATTCATGAAGAATATAGTAAAACAGGGGATAATGATTATTCTATCAAAGTA
  GGATTGAAAGAAAGCGGTCCTGTTATTGTAGCAGCTGCATTAATTATGTTTAGTGTCTTC
  TTTGCGTTTGTATTCCAAGAAGATGTCATGATTAAATCAATGGGTATGGCATTAGCGTTT
  GGTGTACTCTTTGATGCCTTCGTAGTCAGAATGATGTTAATTCCTGCATTAACGAAATTG
  TTTGGAAAAGGTTCGTGGTATTTACCAGCATGGTTGAATCGCATTATTCCACGAGTTGAT
  ATTGAAGGACATGCACTTGAAAAATATAAAACGGTTGAATCACAGGAATCTGAAGCTAAA
  GATAGTAAGGAAACATACGACACAACCTTTAAAGTATATCCACAAGGTGCAACAAATGTA
  TCGAAACATCAAGATGCACATGGGCAAGATGATGCACATTCAATAGTACTAGATGATAAA
  ACGATGGCATTGTACCAAGAAGTGAAACAGCAATCAGCAAGTTCATTGTTCTTATATGAT
  GCTTTAATAGATTATCAAAACAAGCATCAACTAAATTCAAAAAAACAAGCAACGAATATA
  GAACAGTTGAATAAAAATATTGAAAAGCTCAATCAATTGTTAGAAAAAAATCTACGAAAT
  AAATCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
• symbol: SAUSA300_2489
• description: antibiotic transport-associated protein-like protein
• length: 822
• theoretical pI: 7.09563
• theoretical MW: 90408.7
• GRAVY: 0.158273

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 225.2)
  and 5 more
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 121.3)
  hopanoid biosynthesis associated RND transporter like protein HpnN (TIGR03480; HMM-score: 60.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 8.8)
  transmembrane receptor Patched (TIGR00918; HMM-score: 5)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecD (TIGR01129; HMM-score: 4.3)
• TheSEED  :
  • Putative antibiotic transport-associated protein
  CBSS-196620.1.peg.2477  Putative antibiotic transport-associated protein
• PFAM:
  RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 358.2)
  and 2 more
  no clan defined PAP_fibrillin; PAP_fibrillin (PF04755; HMM-score: 11.8)
  Fez1; Fez1 (PF06818; HMM-score: 8.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 31 aa
  • SP(Sec/SPI): 0.713169
  • TAT(Tat/SPI): 0.006206
  • LIPO(Sec/SPII): 0.045188
  • Cleavage Site: CS pos: 31-32. TNA-PK. Pr: 0.2643
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87162338 NCBI
• RefSeq: YP_495123 NCBI
• UniProt: A0A0H2XIZ6 UniProt

⊟Protein sequence

• MGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDNDIKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKKDDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNVQTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGIISLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAGSAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRIKVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPADSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDIVTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVINIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLVMQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYSIKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIPALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVYPQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQLNSKKQATNIEQLNKNIEKLNQLLEKNLRNKS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

regulated by farR (SAUSA300_2490)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications