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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2590 [new locus tag: SACOL_RS13565 ]
  • pan locus tag?: SAUPAN006248000
  • symbol: SACOL2590
  • pan gene symbol?: —
  • synonym:
  • product: glyoxalase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2590 [new locus tag: SACOL_RS13565 ]
  • symbol: SACOL2590
  • product: glyoxalase
  • replicon: chromosome
  • strand: -
  • coordinates: 2653392..2653769
  • length: 378
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGATTCAATCAATGTGGTTTAATTTGCATGTGCAAGATTTAGAAAAGAGCGCACAGTTT
    TATAAAGCGTTAGGATTTAAAATAAATAGAAACCCACAAATGTTAGATAAAATGGTCGGC
    ATTCAAATAGGTCAAACAACCGTAATTTTAATAGAAAACAAGCATTTTCAAAATGTAAGT
    CAGCAAAGCCTTAATACTGAACCAAATGAAGTGATGATTTCTCTAGGTGTGAACACAAAT
    GAAGAAGTTGACCAGTTGGTGAATAAAGTGAAAGAAGCGGGTGGCACAGTCGTTCAAGAA
    CCAACAGTAAGCCAAGGGTTTTATGGAGCAATGTTCAAAGATCTTGATGGACACCATTTT
    AATTTTTTGGTCTGCTAA
    60
    120
    180
    240
    300
    360
    378

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2590 [new locus tag: SACOL_RS13565 ]
  • symbol: SACOL2590
  • description: glyoxalase
  • length: 125
  • theoretical pI: 5.54363
  • theoretical MW: 14160.1
  • GRAVY: -0.2224

⊟Function[edit | edit source]

  • TIGRFAM:
    3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 23.8)
    Metabolism Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 23)
    Metabolism Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 23)
  • TheSEED  :
    • hypothetical protein
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 32.9)
    and 6 more
    Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 14.3)
    3-dmu-9_3-mt; 3-demethylubiquinone-9 3-methyltransferase (PF06983; HMM-score: 13.6)
    no clan defined Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 13.2)
    BRCT-like (CL0459) BRCT; BRCA1 C Terminus (BRCT) domain (PF00533; HMM-score: 13)
    Peptidase_MH (CL0035) Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 12.7)
    P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002114
    • TAT(Tat/SPI): 0.000315
    • LIPO(Sec/SPII): 0.000431
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIGQTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPTVSQGFYGAMFKDLDGHHFNFLVC

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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