From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2686 [new locus tag: SACOL_RS14060 ]
  • pan locus tag?: SAUPAN006408000
  • symbol: cap1B
  • pan gene symbol?: cap1B
  • synonym:
  • product: capsular polysaccharide biosynthesis protein Cap1B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2686 [new locus tag: SACOL_RS14060 ]
  • symbol: cap1B
  • product: capsular polysaccharide biosynthesis protein Cap1B
  • replicon: chromosome
  • strand: -
  • coordinates: 2760376..2761068
  • length: 693
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACGAATACACGAAGAAGTACATCAAGTTTAATTGTCCATGAACAACCAAAGTCACCT
    ATTAGCGAGAAATTTCGAGGCATAAGATCAAATATTATGTTTGCAAATCCTGACAGTGCA
    GTTCAAAGCATTGTAATCACTTCAGAGGCACCAGGCGCAGGTAAGTCTACAATTGCAGCA
    AATTTAGCAGTTGCATATGCGCAAGCAGGTTATAAAACACTAATCGTAGACGGGGATATG
    CGTAAACCTACGCAGCATTATATTTTTAATTTGCCAAACAATGAAGGCCTATCAAGTTTA
    TTGCTAAATTGGTCAACTTATCAAGACAGTATTATCTCAACTGAAATTCAAGATTTAGAC
    GTCTTGACGTCTGGGCCAATCCCACCGAATCCGTCAGAGTTAATTACATCAAGGGCATTT
    GCAAATTTGTATGACACATTATTGATGAATTATAACTTTGTAATTATCGATACGCCACCA
    GTGAACACAGTTACAGATGCGCAATTATTTTCAAAGTTTACCGGCAATGTTGTCTACGTA
    GTTAATTCGGAAAATAATAATAGAGATGAAGTTAAAAAAGGAAAAGAACTTATTGAAGCA
    ACAGGTGCTAAATTATTAGGTGTAGTCTTAAATAGAATGCCTAAAGATAAAAGTGCTAGT
    TACTATGCATATTATGGGACTGATGAATCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2686 [new locus tag: SACOL_RS14060 ]
  • symbol: Cap1B
  • description: capsular polysaccharide biosynthesis protein Cap1B
  • length: 230
  • theoretical pI: 5.30455
  • theoretical MW: 25251.3
  • GRAVY: -0.247391

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids capsular exopolysaccharide family (TIGR01007; HMM-score: 258.7)
    and 17 more
    chain length determinant protein tyrosine kinase EpsG (TIGR03029; HMM-score: 145.5)
    exopolysaccharide/PEP-CTERM locus tyrosine autokinase (TIGR03018; EC 2.7.10.2; HMM-score: 129.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 113.1)
    cell division ATPase MinD (TIGR01969; HMM-score: 55.5)
    Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 40.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides cellulose synthase operon protein YhjQ (TIGR03371; HMM-score: 37.4)
    helicase/secretion neighborhood CpaE-like protein (TIGR03815; HMM-score: 25.7)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogenase iron protein (TIGR01287; EC 1.18.6.1; HMM-score: 17.4)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepA (TIGR03453; HMM-score: 17.4)
    Hypothetical proteins Conserved transport-energizing ATPase, TRC40/GET3/ArsA family (TIGR00345; EC 3.6.1.-; HMM-score: 16.7)
    arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16.3)
    Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 16.2)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 14.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-independent protochlorophyllide reductase, iron-sulfur ATP-binding protein (TIGR01281; EC 1.3.7.7; HMM-score: 13.8)
    signal recognition particle protein SRP54 (TIGR01425; HMM-score: 12.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 11.7)
    HprK-related kinase A (TIGR04352; HMM-score: 10.9)
  • TheSEED  :
    • Capsular polysaccharide synthesis enzyme Cap5B
    • Tyrosine-protein kinase EpsD (EC 2.7.10.2)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Exopolysaccharide Biosynthesis  Tyrosine-protein kinase EpsD (EC 2.7.10.2)
    and 1 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Tyrosine-protein kinase EpsD (EC 2.7.10.2)
  • PFAM:
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 51.7)
    ParA; NUBPL iron-transfer P-loop NTPase (PF10609; HMM-score: 46.5)
    AAA_31; AAA domain (PF13614; HMM-score: 45.8)
    and 14 more
    MipZ; ATPase MipZ (PF09140; HMM-score: 35.6)
    CBP_BcsQ; Cellulose biosynthesis protein BcsQ (PF06564; HMM-score: 27.7)
    ArsA_ATPase; Anion-transporting ATPase (PF02374; HMM-score: 20.1)
    AAA_26; AAA domain (PF13500; HMM-score: 20)
    AAA_25; AAA domain (PF13481; HMM-score: 16.9)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 16.4)
    CLP1_P; mRNA cleavage and polyadenylation factor CLP1 P-loop (PF16575; HMM-score: 15.9)
    SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 14.8)
    Fer4_NifH; 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family (PF00142; HMM-score: 14.6)
    KTI12; Chromatin associated protein KTI12 (PF08433; HMM-score: 12.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.2)
    ArgK; ArgK protein (PF03308; HMM-score: 12)
    AAA_24; AAA domain (PF13479; HMM-score: 12)
    AAA_30; AAA domain (PF13604; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024914
    • TAT(Tat/SPI): 0.011712
    • LIPO(Sec/SPII): 0.002336
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSIVITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLLNWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPPVNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKSASYYAYYGTDES

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2686&oldid=62567"