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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2732 [new locus tag: SACOL_RS14280 ]
  • pan locus tag?: SAUPAN006480000
  • symbol: SACOL2732
  • pan gene symbol?: mbtS
  • synonym:
  • product: transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2732 [new locus tag: SACOL_RS14280 ]
  • symbol: SACOL2732
  • product: transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2801462..2802031
  • length: 570
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGAATCTGAGTAAACAAATTAAAAAGTATAGGGAACGAGATGGTTATTCACAAGAATAT
    CTTGCTGAAAAGTTATATGTATCTAGGCAGAGTATTTCTAATTGGGAAAATGACAAAAGC
    TTACCAGACATACATAACTTATTAATGATGTGTGAATTGTTCAATGTAACTTTAGATGAT
    TTAGTAAAAGGGACCATTCCATTTGTACCTGATATTAAAGCGCAACGAAGTCTTAACTTA
    TGGACATATGTGATGCTTATTTTCATGACATTAGCTGCAATTTTAATGGGACCTTTAGTT
    GTTTATTGGAATTGGACTTGGGGTGTAACGGTGGCAATCATTTTGGGAATAGGTTTTTAT
    GCATCTATGAAAATAGAAGATTTAAAAAAAGTGCATAAAATGGACAACTACGATCGAATT
    GTTGCTTTTATGAATGGAAAAGATCCTAGTGAAGTACAAACGACTAAAGCTAGAAATACG
    ATGACAAATGCGCTTTCTATTATATCAGTAATTGGTATACTCAGCCTCATAATTTTCCTT
    AGTGTGTATTTGGCAAATAAGTTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    570

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2732 [new locus tag: SACOL_RS14280 ]
  • symbol: SACOL2732
  • description: transcriptional regulator
  • length: 189
  • theoretical pI: 9.20529
  • theoretical MW: 21756.5
  • GRAVY: 0.18254

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 36.8)
    and 3 more
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 20.7)
    Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 20.6)
    integral membrane protein (TIGR04561; HMM-score: 7.8)
  • TheSEED  :
    • Transcriptional regulator, XRE family
  • PFAM:
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 61.5)
    and 7 more
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 41.8)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 31.2)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 23.3)
    YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 16.7)
    IT (CL0182) DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 16.6)
    HTH (CL0123) Phage_CI_repr; Bacteriophage CI repressor helix-turn-helix domain (PF07022; HMM-score: 16.3)
    HTH_11; HTH domain (PF08279; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005516
    • TAT(Tat/SPI): 0.000335
    • LIPO(Sec/SPII): 0.001107
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDIHNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVYWNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNTMTNALSIISVIGILSLIIFLSVYLANKFL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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