PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS00400 [old locus tag: SACOL0078 ]
• pan locus tag^?: SAUPAN000858000
• symbol: SACOL_RS00400
• pan gene symbol^?: plc
• synonym:
• product: 1-phosphatidylinositol phosphodiesterase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00400 [old locus tag: SACOL0078 ]
• symbol: SACOL_RS00400
• product: 1-phosphatidylinositol phosphodiesterase
• replicon: chromosome
• strand: +
• coordinates: 88187..89173
• length: 987
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (88187..89173) NCBI
• BioCyc: SACOL_RS00400 BioCyc
• MicrobesOnline: see SACOL0078

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGAAAAAGTGTATTAAGACTTTGTTTTTAAGTATCATTTTAGTAGTGATGAGTGGTTGG
  TATCATTCAGCACATGCGTCAGATTCGTTGAGTAAAAGTCCAGAAAATTGGATGAGTAAA
  CTTGATGATGGAAAACATTTAACTGAGATTAATATACCGGGTTCACATGATAGTGGCTCA
  TTCACTTTAAAGGATCCAGTAAAATCAGTTTGGGCAAAGACTCAAGATAAAGATTACCTT
  ACCCAAATGAAGTCGGGAGTCAGGTTTTTTGATATTAGAGGTAGAGCAAGTGCTGATAAT
  ATGATTTCAGTTCATCACGGCATGGTTTATTTGCATCATGAATTAGGAAAATTTCTCGAT
  GATGCTAAATATTACTTGAGTGCTTATCCAAACGAAACAATTGTGATGTCTATGAAAAAG
  GACTACGATAGCGATTCTAAAGTTACGAAGACATTTGAAGAAATTTTTAGAGAATATTAT
  TATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTA
  AAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGT
  GGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAA
  ATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAA
  AAAGTTGAAGCTGTTAAAAATTTATTGGCTAAAGCTAAAACGGATAGTAACAAAGACAAT
  GTATATGTGAATTTCTTGAGTGTAGCGTCTGGAGGCAGCGCATTTAATAGTACTTATAAC
  TATGCATCACATATAAATCCTGAAATTGCAAAAACGATTAAAGCAAATGGGAAAGCTAGA
  ACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTA
  AGTGAAATTATAGATAGTAATAAATAA
  60
  120
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  420
  480
  540
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  840
  900
  960
  987
  

⊟Protein

• 3V16
  PDB
• 3V18
  PDB
• 3V1H
  PDB
• 4F2B
  PDB
• 4F2T
  PDB
• 4F2U
  PDB
• 4I8Y
  PDB
• 4I90
  PDB
• 4I9J
  PDB
• 4I9M
  PDB
• 4I9T
  PDB
• 4RV3
  PDB
• 4S3G
  PDB

Protein Data Bank: 3V16

Protein Data Bank: 3V18

Protein Data Bank: 3V1H

Protein Data Bank: 4F2B

Protein Data Bank: 4F2T

Protein Data Bank: 4F2U

Protein Data Bank: 4I8Y

Protein Data Bank: 4I90

Protein Data Bank: 4I9J

Protein Data Bank: 4I9M

Protein Data Bank: 4I9T

Protein Data Bank: 4RV3

Protein Data Bank: 4S3G

⊟General

• locus tag: SACOL_RS00400 [old locus tag: SACOL0078 ]
• symbol: SACOL_RS00400
• description: 1-phosphatidylinositol phosphodiesterase
• length: 328
• theoretical pI: 8.62785
• theoretical MW: 37086.4
• GRAVY: -0.555488

⊟Function

• reaction:
  EC 4.6.1.13?  ExPASy
  Phosphatidylinositol diacylglycerol-lyase 1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
• TIGRFAM:
• TheSEED: see SACOL0078
• PFAM:
  PLC (CL0384) PI-PLC-X; Phosphatidylinositol-specific phospholipase C, X domain (PF00388; HMM-score: 65.7)
  and 1 more
  Peptidase_CD (CL0093) Raptor_N; Raptor N-terminal CASPase like domain (PF14538; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.994497
  • TAT(Tat/SPI): 0.000816
  • LIPO(Sec/SPII): 0.003034
  • Cleavage Site: CS pos: 26-27. AHA-SD. Pr: 0.9839
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446633230 NCBI
• RefSeq: WP_000710576 NCBI
• UniProt: see SACOL0078

⊟Protein sequence

• MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDGKHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFETKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFNSTYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK

⊟Experimental data

• experimentally validated: see SACOL0078
• protein localization: see SACOL0078
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications