PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS00865 [old locus tag: SACOL0170 ]
• pan locus tag^?: SAUPAN001025000
• symbol: SACOL_RS00865
• pan gene symbol^?: argD
• synonym:
• product: ornithine aminotransferase 1

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00865 [old locus tag: SACOL0170 ]
• symbol: SACOL_RS00865
• product: ornithine aminotransferase 1
• replicon: chromosome
• strand: -
• coordinates: 194970..196154
• length: 1185
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (194970..196154) NCBI
• BioCyc: SACOL_RS00865 BioCyc
• MicrobesOnline: see SACOL0170

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATTCAATCATTGAATTAACTGATTATTATAGCTCTAATAATTATGCACCACTTAAG
  CTTGTCATTTCTAAAGGTAAAGGTGTCAAAGTTTGGGATACTGATGGCAAACAATATATA
  GATTGCATTTCGGGTTTTTCAGTTGCAAACCAAGGCCATTGTCATCCAACAATTGTTAAA
  GCGATGACAGAACAAGCTTCAAAGTTGTCTATCATTTCACGTGTCCTTTATAGTGACAAT
  CTCGGGAAATGGGAAGAAAAAATTTGTCATCTTGCTAAGAAAGACAAAGTACTCCCCCTT
  AACTCTGGTACTGAAGCTGTTGAAGCAGCCATTAAAATTGCTAGAAAATGGGGCTCTGAA
  GTTAAAGGCATTACTGACGGACAAGTTGAAATCATCGCTATGAATAACAATTTTCACGGT
  CGTACACTTGGCTCATTATCACTATCTAACCACGACGCATATAAAGCAGGATTTCACCCC
  CTACTTCAAGGCACTACAACAGTAGATTTTGGAGACATTGAACAATTAACACAAGCTATT
  TCACCGAATACAGCAGCAATTATTTTGGAACCAATTCAAGGTGAAGGTGGCGTTAATATA
  CCACCGAAAGGATATATTCAAGCTGTGCGTCAACTATGTGATAAACATCAAATATTATTG
  ATTGCAGATGAAATTCAAGTTGGTCTTGGTAGAACTGGGAAATGGTTTGCTATGGAATGG
  GAGCAAGTCGTTCCAGACATTTATATTTTAGGTAAGGCATTGGGTGGCGGCTTATACCCT
  GTATCTGCTGTACTTGCAAATAATGATGTCATGCGTGTTCTAACACCAGGTACACATGGT
  TCAACATTTGGTGGTAACCCTTTAGCCATTGCAATATCGACGGCAGCGCTTGATGTACTT
  AAAGATGAACAACTGGTTGAACGATCAGAACGCTTAGGTTCATTTTTATTAAAAGCGTTG
  CTACAACTTAAACATCCTAGTATTAAAGAAATTAGAGGTCGTGGTTTATTTATAGGCATA
  GAGCTTAACACAGATGCTGCACCTTTTGTGGATCAACTGATTCAACGTGGAATCTTATGC
  AAAGACACGCATCGTACTATCATTCGATTGTCTCCACCTCTAGTCATTGATAAAGAGGAA
  ATCCATCAAATTGTTGCAGCTTTTCAAGACGTTTTTAAAAATTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS00865 [old locus tag: SACOL0170 ]
• symbol: SACOL_RS00865
• description: ornithine aminotransferase 1
• length: 394
• theoretical pI: 7.10134
• theoretical MW: 43048.3
• GRAVY: -0.0327411

⊟Function

• reaction:
  EC 2.6.1.13?  ExPASy
  Ornithine aminotransferase L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid
• TIGRFAM:
  ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 486.8)
  transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 424.9)
  and 13 more
  Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 328)
  Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 271.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 262.1)
  Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 247.9)
  Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 216.3)
  Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 198.2)
  L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 160.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 145.5)
  Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 109.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 14.4)
  tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 14.1)
  Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 13.4)
  Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 12.3)
• TheSEED: see SACOL0170
• PFAM:
  PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 430.9)
  and 5 more
  Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 21.6)
  DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 20.4)
  Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 18.1)
  Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 15.1)
  Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.132451
  • TAT(Tat/SPI): 0.000497
  • LIPO(Sec/SPII): 0.001728
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447007180 NCBI
• RefSeq: WP_001084436 NCBI
• UniProt: see SACOL0170

⊟Protein sequence

• MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCISGFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNSGTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHPLLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQILLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTPGTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGRGLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVFKN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: ArgR/AhrC see SACOL0170

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications