From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03530 [old locus tag: SACOL0686 ]
  • pan locus tag?: SAUPAN002501000
  • symbol: SACOL_RS03530
  • pan gene symbol?: mnhG2
  • synonym:
  • product: cation:proton antiporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03530 [old locus tag: SACOL0686 ]
  • symbol: SACOL_RS03530
  • product: cation:proton antiporter
  • replicon: chromosome
  • strand: +
  • coordinates: 708981..709439
  • length: 459
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (708981..709439) NCBI
  • BioCyc: SACOL_RS03530 BioCyc
  • MicrobesOnline: see SACOL0686

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGGTGGGGGGCATGTGTTTAATGGAAATAACAAAAGAAATCTTTAGTCTTATTGCTGCT
    GTGATGTTGTTGTTAGGTAGTTTTATTGCTCTTATTAGTGCAATAGGTATCGTGAAATTC
    CAAGATGTTTTCTTAAGAAGTCACGCTGCGACAAAAAGTTCAACTTTATCCGTGTTATTA
    ACTTTAATCGGTGTATTAATTTATTTTATTGTGAATACAGGATTTTTCAGTGTGCGTTTA
    TTACTGTCACTTGTTTTTATTAATTTAACTTCACCAGTCGGCATGCACTTAGTCGCTCGC
    GCTGCTTATCGCAACGGCGCTTATATGTATCGAAAAAATGATGCTCACACACATGCATCA
    ATATTATTAAGTTCAAATGAACAAAACTCTACAGAAGCATTACAATTACGTGCTGAAAAA
    CGAGAAGAGCATCGTAAGAAATGGTATCAAAACGATTGA
    60
    120
    180
    240
    300
    360
    420
    459

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03530 [old locus tag: SACOL0686 ]
  • symbol: SACOL_RS03530
  • description: cation:proton antiporter
  • length: 152
  • theoretical pI: 9.97435
  • theoretical MW: 16994.9
  • GRAVY: 0.417763

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation/proton antiporter, MnhG/PhaG subunit (TIGR01300; HMM-score: 89.9)
    and 1 more
    exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 15.9)
  • TheSEED: see SACOL0686
  • PFAM:
    no clan defined PhaG_MnhG_YufB; Na+/H+ antiporter subunit (PF03334; HMM-score: 78.4)
    and 4 more
    CCB1; Cofactor assembly of complex C subunit B (PF12046; HMM-score: 15.8)
    DUF4029; Protein of unknown function (DUF4029) (PF13221; HMM-score: 9.8)
    DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 8.7)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 6.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002441
    • TAT(Tat/SPI): 0.000197
    • LIPO(Sec/SPII): 0.008848
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVGGMCLMEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAATKSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYMYRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS03530&oldid=87838"