PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS07130 [old locus tag: SACOL1401 ]
• pan locus tag^?: SAUPAN003758000
• symbol: SACOL_RS07130
• pan gene symbol^?: tyrA
• synonym:
• product: prephenate dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07130 [old locus tag: SACOL1401 ]
• symbol: SACOL_RS07130
• product: prephenate dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 1411431..1412522
• length: 1092
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1411431..1412522) NCBI
• BioCyc: SACOL_RS07130 BioCyc
• MicrobesOnline: see SACOL1401

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
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  661
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  841
  901
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  1021
  1081
  
  ATGACAACAGTTTTATTTGTTGGGCTTGGATTAATTGGTGGAAGTCTTGCTAGCAATATA
  AAATACCATAACCCTAATACTAATATTATTGCATACGATGCAGATACTTCTCAGTTAGAT
  AAAGCTAAATCAATCGGCATTATTAATGAAAAATGTTTAAATTATAGTGAAGCTATTAAA
  AAAGCCGATGTAATTATTTATGCAACACCTGTTGCTATCACAAATAAATATCTTAGCGAG
  CTTATAGATATGCCAACTAAACCTGGTGTTATTGTTTCTGATACTGGTAGTACTAAAGCA
  ATGATACAGCAACACGAATGCAATTTATTAAAGCATAATATTCATTTAGTCAGTGGTCAT
  CCAATGGCTGGTAGTCATAAATCTGGTGTACTAAATGCTAAAAAGCACTTATTTGAAAAC
  GCTTATTATATTTTAGTCTACAATGAGCCAAGAAATGAGCAAGCAGCAAACACGTTAAAA
  GAACTGTTATCACCTACTCTTGCTAAATTTATTGTAACTACTGCTGAAGAACACGACTAC
  GTAACAAGCGTCGTAAGTCATTTACCTCATATCGTTGCATCTAGTTTAGTTCATGTTAGT
  CAAAAGAACGGTCAAGAACATCATTTAGTTAATAAACTTGCAGCTGGTGGTTTTCGTGAT
  ATCACTCGTATAGCTAGTAGTAATGCACAAATGTGGAAAGATATCACCTTGAGTAATAAA
  ACGTATATTTTAGAAATGATTCGACAGCTAAAAAGTCAGTTTCAAGATTTAGAAAGACTA
  ATTGAAAGCAATGATTCTGAAAAATTGTTATCATTTTTTGCCCAAGCTAAATCGTATCGC
  GACGCACTACCCGCTAAACAACTAGGTGGACTAAATACTGCGTATGATCTATATGTAGAC
  ATTCCGGATGAATCAGGTATGATAAGTAAAGTGACTTATATCCTGAGTTTACATAACATA
  TCTATAAGCAACTTAAGAATCTTAGAAGTACGCGAAGATATATACGGTGCTTTAAAAATT
  AGTTTCAAAAACCCTACTGACCGAGAACGCGGTATGCAAGCATTGAGTGATTTTGATTGT
  TATATCCAATAA
  60
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  1080
  1092
  

⊟Protein

⊟General

• locus tag: SACOL_RS07130 [old locus tag: SACOL1401 ]
• symbol: SACOL_RS07130
• description: prephenate dehydrogenase
• length: 363
• theoretical pI: 7.22968
• theoretical MW: 40394.9
• GRAVY: -0.167493

⊟Function

• reaction:
  EC 1.3.1.12?  ExPASy
  Prephenate dehydrogenase Prephenate + NAD⁺ = 4-hydroxyphenylpyruvate + CO₂ + NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 23.8)
  Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 21.7)
  Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 19.3)
  and 3 more
  Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 17)
  Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 15.3)
  Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 12.3)
• TheSEED: see SACOL1401
• PFAM:
  NADP_Rossmann (CL0063) PDH; Prephenate dehydrogenase (PF02153; HMM-score: 233)
  and 10 more
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 31.8)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29)
  ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 20.4)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 19.7)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 17.2)
  GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 16.4)
  no clan defined P4Ha_N; Prolyl 4-Hydroxylase alpha-subunit, N-terminal region (PF08336; HMM-score: 14.2)
  NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 13.5)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13)
  NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.408934
  • TAT(Tat/SPI): 0.003896
  • LIPO(Sec/SPII): 0.132579
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446136411 NCBI
• RefSeq: WP_000214266 NCBI
• UniProt: see SACOL1401

⊟Protein sequence

• MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKSIGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMIQQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLKELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGFRDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQAKSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDIYGALKISFKNPTDRERGMQALSDFDCYIQ

⊟Experimental data

• experimentally validated: see SACOL1401
• protein localization: see SACOL1401
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SACOL1401

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications