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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1401 [new locus tag: SACOL_RS07130 ]
  • pan locus tag?: SAUPAN003758000
  • symbol: tyrA
  • pan gene symbol?: tyrA
  • synonym:
  • product: prephenate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1401 [new locus tag: SACOL_RS07130 ]
  • symbol: tyrA
  • product: prephenate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1411431..1412522
  • length: 1092
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACAACAGTTTTATTTGTTGGGCTTGGATTAATTGGTGGAAGTCTTGCTAGCAATATA
    AAATACCATAACCCTAATACTAATATTATTGCATACGATGCAGATACTTCTCAGTTAGAT
    AAAGCTAAATCAATCGGCATTATTAATGAAAAATGTTTAAATTATAGTGAAGCTATTAAA
    AAAGCCGATGTAATTATTTATGCAACACCTGTTGCTATCACAAATAAATATCTTAGCGAG
    CTTATAGATATGCCAACTAAACCTGGTGTTATTGTTTCTGATACTGGTAGTACTAAAGCA
    ATGATACAGCAACACGAATGCAATTTATTAAAGCATAATATTCATTTAGTCAGTGGTCAT
    CCAATGGCTGGTAGTCATAAATCTGGTGTACTAAATGCTAAAAAGCACTTATTTGAAAAC
    GCTTATTATATTTTAGTCTACAATGAGCCAAGAAATGAGCAAGCAGCAAACACGTTAAAA
    GAACTGTTATCACCTACTCTTGCTAAATTTATTGTAACTACTGCTGAAGAACACGACTAC
    GTAACAAGCGTCGTAAGTCATTTACCTCATATCGTTGCATCTAGTTTAGTTCATGTTAGT
    CAAAAGAACGGTCAAGAACATCATTTAGTTAATAAACTTGCAGCTGGTGGTTTTCGTGAT
    ATCACTCGTATAGCTAGTAGTAATGCACAAATGTGGAAAGATATCACCTTGAGTAATAAA
    ACGTATATTTTAGAAATGATTCGACAGCTAAAAAGTCAGTTTCAAGATTTAGAAAGACTA
    ATTGAAAGCAATGATTCTGAAAAATTGTTATCATTTTTTGCCCAAGCTAAATCGTATCGC
    GACGCACTACCCGCTAAACAACTAGGTGGACTAAATACTGCGTATGATCTATATGTAGAC
    ATTCCGGATGAATCAGGTATGATAAGTAAAGTGACTTATATCCTGAGTTTACATAACATA
    TCTATAAGCAACTTAAGAATCTTAGAAGTACGCGAAGATATATACGGTGCTTTAAAAATT
    AGTTTCAAAAACCCTACTGACCGAGAACGCGGTATGCAAGCATTGAGTGATTTTGATTGT
    TATATCCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1401 [new locus tag: SACOL_RS07130 ]
  • symbol: TyrA
  • description: prephenate dehydrogenase
  • length: 363
  • theoretical pI: 7.22968
  • theoretical MW: 40394.9
  • GRAVY: -0.167493

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.1.12?  ExPASy
    Prephenate dehydrogenase Prephenate + NAD+ = 4-hydroxyphenylpyruvate + CO2 + NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 23.8)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 21.7)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 19.3)
    and 3 more
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 17)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 15.3)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 12.3)
  • TheSEED  :
    • Prephenate dehydrogenase (EC 1.3.1.12)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  Prephenate dehydrogenase (EC 1.3.1.12)
    and 1 more
    Amino Acids and Derivatives Aromatic amino acids and derivatives Phenylalanine and Tyrosine Branches from Chorismate  Prephenate dehydrogenase (EC 1.3.1.12)
  • PFAM:
    NADP_Rossmann (CL0063) PDH; Prephenate dehydrogenase (PF02153; HMM-score: 233)
    and 10 more
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 31.8)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 20.4)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 19.7)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 17.2)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 16.4)
    no clan defined P4Ha_N; Prolyl 4-Hydroxylase alpha-subunit, N-terminal region (PF08336; HMM-score: 14.2)
    NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 13.5)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.5
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.408934
    • TAT(Tat/SPI): 0.003896
    • LIPO(Sec/SPII): 0.132579
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKSIGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMIQQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLKELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGFRDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQAKSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDIYGALKISFKNPTDRERGMQALSDFDCYIQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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