PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS07175 [old locus tag: SACOL1410 ]
• pan locus tag^?: SAUPAN003769000
• symbol: SACOL_RS07175
• pan gene symbol^?: femA
• synonym:
• product: aminoacyltransferase FemA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07175 [old locus tag: SACOL1410 ]
• symbol: SACOL_RS07175
• product: aminoacyltransferase FemA
• replicon: chromosome
• strand: +
• coordinates: 1420849..1422111
• length: 1263
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1420849..1422111) NCBI
• BioCyc: SACOL_RS07175 BioCyc
• MicrobesOnline: see SACOL1410

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGAAGTTTACAAATTTAACAGCTAAAGAGTTTGGTGCCTTTACAGATAGCATGCCATAC
  AGTCATTTCACGCAAACTGTTGGCCACTATGAGTTAAAGCTTGCTGAAGGTTATGAAACA
  CATTTAGTGGGAATAAAAAACAATAATAACGAGGTCATTGCAGCTTGCTTACTTACTGCT
  GTACCTGTTATGAAAGTGTTCAAGTATTTTTATTCAAATCGCGGTCCAGTGATTGATTAT
  GAAAATCAAGAACTCGTACACTTTTTCTTTAATGAATTATCAAAATATGTTAAAAAACAT
  CGTTGTCTATACCTACATATCGATCCATATTTACCATATCAATACTTGAATCATGATGGC
  GAGATTACAGGTAATGCTGGTAATGATTGGTTCTTTGATAAAATGAGTAACTTAGGATTT
  GAACATACTGGATTCCATAAAGGATTTGATCCTGTGCTACAAATTCGTTATCACTCAGTG
  TTAGATTTAAAAGATAAAACAGCAGATGACATCATTAAAAATATGGATGGACTTAGAAAA
  AGAAACACGAAAAAAGTTAAAAAGAATGGTGTTAAAGTAAGATTTTTATCTGAAGAAGAA
  CTACCAATTTTTAGATCATTTATGGAAGATACGTCAGAATCAAAAGCTTTTGCTGATCGT
  GATGACAAATTTTACTACAATCGCTTAAAATATTACAAAGACCGTGTGTTAGTACCTTTA
  GCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAAT
  AAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACAT
  AACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGT
  AAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATC
  AATCCATTTGAAGTTGTTTATTATGCTGGTGGTACATCAAATGCATTCCGTCATTTTGCC
  GGAAGTTATGCAGTGCAATGGGAAATGATTAATTATGCATTAAATCATGGCATTGACCGT
  TATAATTTCTATGGTGTTAGTGGTAAATTTACAGAAGATGCTGAAGATGCTGGTGTAGTT
  AAATTCAAAAAAGGTTACAATGCTGAAATTATTGAATATGTTGGTGACTTTATTAAACCA
  ATTAATAAACCTGTTTACGCAGCATATACCGCACTTAAAAAAGTTAAAGACAGAATTTTT
  TAG
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  960
  1020
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  1140
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  1260
  1263
  

⊟Protein

⊟General

• locus tag: SACOL_RS07175 [old locus tag: SACOL1410 ]
• symbol: SACOL_RS07175
• description: aminoacyltransferase FemA
• length: 420
• theoretical pI: 7.48443
• theoretical MW: 49123.3
• GRAVY: -0.614762

⊟Function

• reaction:
  EC 2.3.2.17?  ExPASy
  N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N⁶-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N⁶-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNA = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N⁶-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation serine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 7.5)
  and 1 more
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage major capsid protein, HK97 family (TIGR01554; HMM-score: 4.1)
• TheSEED: see SACOL1410
• PFAM:
  Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 509)
  and 3 more
  Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 16.2)
  no clan defined FlxA; FlxA-like protein (PF14282; HMM-score: 11.5)
  FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 7.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 10
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012423
  • TAT(Tat/SPI): 0.000339
  • LIPO(Sec/SPII): 0.000745
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446595963 NCBI
• RefSeq: WP_000673309 NCBI
• UniProt: see SACOL1410

⊟Protein sequence

• MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVGIKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRCLYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSVLDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFADRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPENKKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSNAFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIEYVGDFIKPINKPVYAAYTALKKVKDRIF

⊟Experimental data

• experimentally validated: see SACOL1410
• protein localization: see SACOL1410
• quantitative data / protein copy number per cell: see SACOL1410
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications