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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1269 [new locus tag: SAUSA300_RS06890 ]
  • pan locus tag?: SAUPAN003769000
  • symbol: femA
  • pan gene symbol?: femA
  • synonym:
  • product: methicillin resistance protein FemA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1269 [new locus tag: SAUSA300_RS06890 ]
  • symbol: femA
  • product: methicillin resistance protein FemA
  • replicon: chromosome
  • strand: +
  • coordinates: 1397007..1398308
  • length: 1302
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAATTAATTAACGAGAGACAAATAGGAGTAATGATAATGAAGTTTACAAATTTAACA
    GCTAAAGAGTTTGGTGCCTTTACAGATAGCATGCCATACAGTCATTTCACGCAAACTGTT
    GGCCACTATGAGTTAAAGCTTGCTGAAGGTTATGAAACACATTTAGTGGGAATAAAAAAC
    AATAATAACGAGGTCATTGCAGCTTGCTTACTTACTGCTGTACCTGTTATGAAAGTGTTC
    AAGTATTTTTATTCAAATCGCGGTCCAGTGATTGATTATGAAAATCAAGAACTCGTACAC
    TTTTTCTTTAATGAATTATCAAAATATGTTAAAAAACATCGTTGTCTATACCTACATATC
    GATCCATATTTACCATATCAATACTTGAATCATGATGGCGAGATTACAGGTAATGCTGGT
    AATGATTGGTTCTTTGATAAAATGAGTAACTTAGGATTTGAACATACTGGATTCCATAAA
    GGATTTGATCCTGTGCTACAAATTCGTTATCACTCAGTGTTAGATTTAAAAGATAAAACA
    GCAGATGACATCATTAAAAATATGGATGGACTTAGAAAAAGAAACACGAAAAAAGTTAAA
    AAGAATGGTGTTAAAGTAAGATTTTTATCTGAAGAAGAACTACCAATTTTTAGATCATTT
    ATGGAAGATACGTCAGAATCAAAAGCTTTTGCTGATCGTGATGACAAATTTTACTACAAT
    CGCTTAAAATATTACAAAGACCGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAA
    TATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTA
    AAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAA
    CAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACAT
    GGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAGTTGTTTAT
    TATGCTGGTGGTACATCAAATGCATTCCGTCATTTTGCCGGAAGTTATGCAGTGCAATGG
    GAAATGATTAATTATGCATTAAATCATGGCATTGACCGTTATAATTTCTATGGTGTTAGT
    GGTAAATTTACAGAAGATGCTGAAGATGCTGGTGTAGTTAAATTCAAAAAAGGTTACAAT
    GCTGAAATTATTGAATATGTTGGTGACTTTATTAAACCAATTAATAAACCTGTTTACGCA
    GCATATACCGCACTTAAAAAAGTTAAAGACAGAATTTTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1302

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1269 [new locus tag: SAUSA300_RS06890 ]
  • symbol: FemA
  • description: methicillin resistance protein FemA
  • length: 433
  • theoretical pI: 7.8089
  • theoretical MW: 50650.2
  • GRAVY: -0.582448

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage major capsid protein, HK97 family (TIGR01554; HMM-score: 4)
  • TheSEED  :
    • FemA, factor essential for methicillin resistance
    • tRNA-dependent lipid II-Gly--glycine ligase
    • tRNA-dependent lipid II-GlyGly--glycine ligase
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory tRNA-dependent amino acid transfers  tRNA-dependent lipid II-Gly--glycine ligase
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory tRNA-dependent amino acid transfers  tRNA-dependent lipid II-GlyGly--glycine ligase
  • PFAM:
    Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 508.8)
    and 3 more
    Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 16.2)
    no clan defined FlxA; FlxA-like protein (PF14282; HMM-score: 11.4)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 7.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.031114
    • TAT(Tat/SPI): 0.001401
    • LIPO(Sec/SPII): 0.004041
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLINERQIGVMIMKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVGIKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRCLYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSVLDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFADRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPENKKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSNAFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIEYVGDFIKPINKPVYAAYTALKKVKDRIF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

FemA is responsible for the second step in synthesis of the peptidoglycan pentaglycine cross-bridge. It adds a glycyl-glycine to glycyl-lipid-II resulting in a triglycine cross-bridge. When bound to FmhA, FemA can incorporate a glycyl-serine resulting in an altered cross-bridge.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Stephanie Willing, Emma Dyer, Olaf Schneewind, Dominique Missiakas
    FmhA and FmhC of Staphylococcus aureus incorporate serine residues into peptidoglycan cross-bridges.
    J Biol Chem: 2020, 295(39);13664-13676
    [PubMed:32759309] [WorldCat.org] [DOI] (I p)
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