PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS07820 [old locus tag: SACOL1534 ]
• pan locus tag^?: SAUPAN004024000
• symbol: SACOL_RS07820
• pan gene symbol^?: srrB
• synonym:
• product: two-component sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07820 [old locus tag: SACOL1534 ]
• symbol: SACOL_RS07820
• product: two-component sensor histidine kinase
• replicon: chromosome
• strand: -
• coordinates: 1572353..1574104
• length: 1752
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1572353..1574104) NCBI
• BioCyc: SACOL_RS07820 BioCyc
• MicrobesOnline: see SACOL1534

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  
  ATGATGAGCCGGCTAAATAGTGTCGTAATTAAACTGTGGTTAACTATTATTTTAATAGTG
  ACGACAGTTTTAATTTTATTAAGTATTGCTTTAATTACCTTTATGCAATACTATTTCACA
  CAAGAAACCGAAAATGCCATAAGAGAAGATGCTAGACGTATAAGTTCACTGGTCGAACAA
  TCACATAATAAAGAAGAAGCAATAAAATATAGTCAAACATTAATTGAAAATCCTGGTGGG
  TTGATGATTATAAATAATAAACATCGTCAATCAACGGCTTCACTTTCTAATATTAAAAAG
  CAAATGTTGAATGAAGTAGTCAACAACGACCATTTTGACGATGTGTTTGATAAAGGTAAA
  TCTGTTACTCGAAATGTAACGATTAAAGAAAAGGGCTCATCTCAAACATATATTTTGTTA
  GGCTATCCAACAAAAGCACAGAAGAATAGTCATAGCAAATATAGTGGAGTCTTTATATAT
  AAAGACTTGAAATCAATCGAAGATACAAATAATGCTATTACGATTATCACCATAATTACG
  GCTGTTATTTTCTTAACAATTACAACAGTCTTTGCGTTTTTCTTATCGTCAAGAATTACA
  AAACCTTTAAGACGTTTAAGAGACCAAGCTACACGTGTATCTGAAGGGGATTACTCTTAT
  AAACCTTCTGTCACAACGAAAGATGAAATTGGTCAATTATCGCAGGCATTTAATCAGATG
  AGTACAGAAATCGAAGAGCATGTCGACGCATTATCCACATCTAAAAATATTAGAGACAGC
  TTAATTAACTCTATGGTAGAAGGTGTCCTAGGTATTAATGAGAGTCGACAAATTATCTTA
  TCTAATAAGATGGCGAATGATATTATGGACAATATTGATGAAGATGCTAAAGCTTTCTTA
  TTAAGACAAATAGAAGATACTTTTAAATCAAAACAAACTGAAATGCGCGATTTAGAAATG
  AATGCACGATTCTTTGTTGTGACCACAAGCTATATCGACAAGATTGAACAGGGAGGTAAA
  AGTGGTGTTGTTGTGACAGTTCGTGATATGACTAATGAGCACAATCTAGATCAAATGAAG
  AAAGATTTCATTGCTAATGTATCACATGAATTACGTACACCGATATCATTACTTCAAGGT
  TATACTGAATCAATTGTAGATGGTATTGTTACAGAACCGGATGAAATAAAAGAATCGCTT
  GCCATTGTCCTTGATGAATCGAAACGTTTAAATCGTTTAGTTAATGAATTGTTAAATGTC
  GCACGCATGGATGCTGAAGGGTTATCCGTAAATAAAGAAGTTCAGCCTATTGCAGCGTTA
  CTAGATAAGATGAAAATTAAGTATCGCCAACAAGCTGATGATTTAGGTCTAAATATGACT
  TTTAATTATTGTAAGAAGCGTGTTTGGAGTTATGATATGGATCGCATGGACCAAGTACTA
  ACGAACTTAATTGATAATGCATCACGTTATACGAAACCTGGAGATGAAATTGCAATTACT
  TGTGATGAAAATGAAAGCGAAGATATTTTATACATTAAAGATACAGGTACAGGCATTGCA
  CCAGAACATTTACAACAAGTATTTGATCGTTTTTATAAAGTTGATGCAGCGAGAACGCGA
  GGTAAACAAGGTACCGGTTTAGGTTTGTTCATTTGTAAAATGATTATCGAAGAGCATGGT
  GGTTCCATAGATGTTAAAAGCGAATTAGGGAAAGGCACAACATTTATTATTAAACTACCA
  AAACCAGAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1752
  

⊟Protein

⊟General

• locus tag: SACOL_RS07820 [old locus tag: SACOL1534 ]
• symbol: SACOL_RS07820
• description: two-component sensor histidine kinase
• length: 583
• theoretical pI: 5.76595
• theoretical MW: 66075.2
• GRAVY: -0.3

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 242.7)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 199.2)
  and 6 more
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 135)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 95.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.6)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.6)
• TheSEED: see SACOL1534
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 95.1)
  and 18 more
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 64.9)
  no clan defined HAMP; HAMP domain (PF00672; HMM-score: 62.7)
  His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 23.4)
  no clan defined Halogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 18.9)
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.8)
  PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 16.8)
  no clan defined Cep57_MT_bd; Centrosome microtubule-binding domain of Cep57 (PF06657; HMM-score: 14.5)
  EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.6)
  Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 12.6)
  Vpu; Vpu protein (PF00558; HMM-score: 12.3)
  DUF3328; Domain of unknown function (DUF3328) (PF11807; HMM-score: 12.3)
  Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 10.8)
  no clan defined Serinc; Serine incorporator (Serinc) (PF03348; HMM-score: 9)
  PspB; Phage shock protein B (PF06667; HMM-score: 7.9)
  ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 7.6)
  no clan defined TRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 7.2)
  CemA; CemA family (PF03040; HMM-score: 7.1)
  DUF3165; Protein of unknown function (DUF3165) (PF11364; HMM-score: 7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002704
  • TAT(Tat/SPI): 0.000289
  • LIPO(Sec/SPII): 0.00216
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446910513 NCBI
• RefSeq: WP_000987769 NCBI
• UniProt: see SACOL1534

⊟Protein sequence

• MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETENAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQMLNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIYKDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESRQIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESEDILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLPKPE

⊟Experimental data

• experimentally validated: see SACOL1534
• protein localization: see SACOL1534
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: Rex* see SACOL1534

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications