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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01585
  • pan locus tag?: SAUPAN004024000
  • symbol: SAOUHSC_01585
  • pan gene symbol?: srrB
  • synonym:
  • product: respiratory response protein SrrB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01585
  • symbol: SAOUHSC_01585
  • product: respiratory response protein SrrB
  • replicon: chromosome
  • strand: -
  • coordinates: 1511734..1513485
  • length: 1752
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGAGCCGGCTAAATAGTGTCGTAATTAAACTGTGGTTAACTATTATTTTAATAGTG
    ACGACAGTTTTAATTTTATTAAGTATTGCTTTAATTACCTTTATGCAATACTATTTCACA
    CAAGAAACCGAAAATGCCATAAGAGAAGATGCTAGACGTATAAGTTCACTGGTCGAACAA
    TCACATAATAAAGAAGAAGCAATAAAATATAGTCAAACATTAATTGAAAATCCTGGTGGG
    TTGATGATTATAAATAATAAACATCGTCAATCAACGGCTTCACTTTCTAATATTAAAAAG
    CAAATGTTGAATGAAGTAGTCAACAACGACCATTTTGACGATGTGTTTGATAAAGGTAAA
    TCTGTTACTCGAAATGTAACGATTAAAGAAAAGGGCTCATCTCAAACATATATTTTGTTA
    GGCTATCCAACAAAAGCACAGAAGAATAGTCATAGCAAATATAGTGGAGTCTTTATATAT
    AAAGACTTGAAATCAATCGAAGATACAAATAATGCTATTACGATTATCACCATAATTACG
    GCTGTTATTTTCTTAACAATTACAACAGTCTTTGCGTTTTTCTTATCGTCAAGAATTACA
    AAACCTTTAAGACGTTTAAGAGACCAAGCTACACGTGTATCTGAAGGGGATTACTCTTAT
    AAACCTTCTGTCACAACGAAAGATGAAATTGGTCAATTATCGCAGGCATTTAATCAGATG
    AGTACAGAAATCGAAGAGCATGTCGACGCATTATCCACATCTAAAAATATTAGAGACAGC
    TTAATTAACTCTATGGTAGAAGGTGTCCTAGGTATTAATGAGAGTCGACAAATTATCTTA
    TCTAATAAGATGGCGAATGATATTATGGACAATATTGATGAAGATGCTAAAGCTTTCTTA
    TTAAGACAAATAGAAGATACTTTTAAATCAAAACAAACTGAAATGCGCGATTTAGAAATG
    AATGCACGATTCTTTGTTGTGACCACAAGCTATATCGACAAGATTGAACAGGGAGGTAAA
    AGTGGTGTTGTTGTGACAGTTCGTGATATGACTAATGAGCACAATCTAGATCAAATGAAG
    AAAGATTTCATTGCTAATGTATCACATGAATTACGTACACCGATATCATTACTTCAAGGT
    TATACTGAATCAATTGTAGATGGTATTGTTACAGAACCGGATGAAATAAAAGAATCGCTT
    GCCATTGTCCTTGATGAATCGAAACGTTTAAATCGTTTAGTTAATGAATTGTTAAATGTC
    GCACGCATGGATGCTGAAGGGTTATCCGTAAATAAAGAAGTTCAGCCTATTGCAGCGTTA
    CTAGATAAGATGAAAATTAAGTATCGCCAACAAGCTGATGATTTAGGTCTAAATATGACT
    TTTAATTATTGTAAGAAGCGTGTTTGGAGTTATGATATGGATCGCATGGACCAAGTACTA
    ACGAACTTAATTGATAATGCATCACGTTATACGAAACCTGGAGATGAAATTGCAATTACT
    TGTGATGAAAATGAAAGCGAAGATATTTTATACATTAAAGATACAGGTACAGGCATTGCA
    CCAGAACATTTACAACAAGTATTTGATCGTTTTTATAAAGTTGATGCAGCGAGAACGCGA
    GGTAAACAAGGTACCGGTTTAGGTTTGTTCATTTGTAAAATGATTATCGAAGAGCATGGT
    GGTTCCATAGATGTTAAAAGCGAATTAGGGAAAGGCACAACATTTATTATTAAACTACCA
    AAACCAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1752

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01585
  • symbol: SAOUHSC_01585
  • description: respiratory response protein SrrB
  • length: 583
  • theoretical pI: 5.76595
  • theoretical MW: 66075.2
  • GRAVY: -0.3

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 242.7)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 199.2)
    and 6 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 135)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 95.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.6)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.6)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 36.6)
  • TheSEED  :
    • Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 95.1)
    and 18 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 64.9)
    no clan defined HAMP; HAMP domain (PF00672; HMM-score: 62.7)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 23.4)
    no clan defined Halogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 18.9)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.8)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 16.8)
    no clan defined Cep57_MT_bd; Centrosome microtubule-binding domain of Cep57 (PF06657; HMM-score: 14.5)
    EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 13.6)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 12.6)
    Vpu; Vpu protein (PF00558; HMM-score: 12.3)
    DUF3328; Domain of unknown function (DUF3328) (PF11807; HMM-score: 12.3)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 10.8)
    no clan defined Serinc; Serine incorporator (Serinc) (PF03348; HMM-score: 9)
    PspB; Phage shock protein B (PF06667; HMM-score: 7.9)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 7.6)
    no clan defined TRP; Transient receptor potential (TRP) ion channel (PF06011; HMM-score: 7.2)
    CemA; CemA family (PF03040; HMM-score: 7.1)
    DUF3165; Protein of unknown function (DUF3165) (PF11364; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002704
    • TAT(Tat/SPI): 0.000289
    • LIPO(Sec/SPII): 0.00216
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETENAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQMLNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIYKDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDYSYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESRQIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDKIEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTEPDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQQADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESEDILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVKSELGKGTTFIIKLPKPE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Rex* (repression) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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