From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08935 [old locus tag: SACOL1746 ]
  • pan locus tag?: SAUPAN004325000
  • symbol: SACOL_RS08935
  • pan gene symbol?: pfkA
  • synonym:
  • product: ATP-dependent 6-phosphofructokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08935 [old locus tag: SACOL1746 ]
  • symbol: SACOL_RS08935
  • product: ATP-dependent 6-phosphofructokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1783705..1784673
  • length: 969
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1783705..1784673) NCBI
  • BioCyc: SACOL_RS08935 BioCyc
  • MicrobesOnline: see SACOL1746

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGAAAATTGCAGTTTTAACTAGTGGTGGAGATTCACCTGGAATGAATGCTGCCGTA
    AGAGCAGTTGTTCGTACAGCAATTTACAATGAAATTGAAGTTTATGGTGTGTATCATGGT
    TACCAAGGATTGTTAAATGATGATATTCATAAACTTGAATTAGGATCAGTTGGGGATACG
    ATTCAGCGTGGAGGTACATTCTTGTATTCAGCAAGATGTCCAGAGTTTAAGGAGCAAGAA
    GTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAGGGCCTTGTAGTTATT
    GGTGGTGACGGTAGTTATCGCGGTGCACAACGCATCAGTGAGGAATGTAAAGAAATTCAA
    ACTATCGGTATTCCTGGTACGATTGACAATGATATCAATGGTACTGATTTTACAATTGGA
    TTTGACACAGCATTAAATACGATTATTGGCTTAGTCGACAAAATTAGAGATACTGCGTCA
    AGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGTGGAGATCTAGCATTA
    TGGGCTGGATTATCAGTTGGTGCTGAGACAATTGTAGTTCCAGAAGTGAAAACAGATATT
    AAAGAAATAGCTGATAAAATTGAACAAGGTATTAAACGTGGTAAGAAACACTCAATCGTT
    CTTGTAGCAGAAGGTTGTATGACTGCGCAAGATTGTCAAAAAGAATTATCACAATACATC
    AATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGTGGTAGCCCAACAGGT
    GCGGATAGAGTTTTAGCATCACGTTTAGGTGGATATGCGGTAGACTTATTAATGCAAGGT
    GAAACAGCTAAGGGTGTTGGAATTAAGAACAATAAAATTGTAGCAACATCTTTTGATGAA
    ATTTTTGATGGTAAAGATCATAAATTTGATTATAGTCTATATGAACTTGCTAACAAGTTA
    TCTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS08935 [old locus tag: SACOL1746 ]
  • symbol: SACOL_RS08935
  • description: ATP-dependent 6-phosphofructokinase
  • length: 322
  • theoretical pI: 5.71393
  • theoretical MW: 34839.4
  • GRAVY: -0.160559

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.11?  ExPASy
    6-phosphofructokinase ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis 6-phosphofructokinase (TIGR02482; EC 2.7.1.11; HMM-score: 428)
    and 3 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphofructokinase (TIGR02483; HMM-score: 301.2)
    6-phosphofructokinase (TIGR02478; EC 2.7.1.11; HMM-score: 295.8)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis diphosphate--fructose-6-phosphate 1-phosphotransferase (TIGR02477; EC 2.7.1.90; HMM-score: 82.2)
  • TheSEED: see SACOL1746
  • PFAM:
    PFK (CL0240) PFK; Phosphofructokinase (PF00365; HMM-score: 367.1)
    and 2 more
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 13.1)
    DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030571
    • TAT(Tat/SPI): 0.000327
    • LIPO(Sec/SPII): 0.001795
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS08935&oldid=84475"