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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS10460 [old locus tag: SACOL2002 ]
  • pan locus tag?: SAUPAN005009000
  • symbol: SACOL_RS10460
  • pan gene symbol?: eap/map
  • synonym:
  • product: MAP domain-containing protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS10460 [old locus tag: SACOL2002 ]
  • symbol: SACOL_RS10460
  • product: MAP domain-containing protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2061583..2063337
  • length: 1755
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (2061583..2063337) NCBI
  • BioCyc: SACOL_RS10460 BioCyc
  • MicrobesOnline: see SACOL2002

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAATTTAAGTCATTGATTACAACAACTTTAGCATTAGGTGTTTTAGCATCAACAGGT
    GCAAACTTTAATAATAATGAAGCGTCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
    TCGTTACACCATGGATATTCTAAAGTCCATGTTCCATATGCAATCACTGTGAACGGTACA
    AGCCAAAATATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
    TTAGAGGATAGAGTTAAATCAGTTTTAAAATCAGACAGAGGTATTAGTGATATAGATTTA
    AGACTATCGAAGCAAGCGAAATATACTGTTTACTTTAAAAATGGAACAAAGAAAGTTATC
    GATTTGAAAGCAGGTATTTACACAGCTGATTTAATTAATACAAGTGAAATTAAAGCTATT
    AATATTAACGTAGATACTAAAAAGCAAGTTGAAGATAAAAAGAAAGATAAAGCAAATTAC
    CAAGTTCCATACACAATCACTGTGAACGGTACAAGCCAAAATATTTTATCAAACTTAACA
    TTTAATAAGAATCAAAATATTAGTTACAAAGATTTAGAGGATAAAGTTAAATCAGTTTTA
    GAATCAAATAGAGGTATTACCGATGTTGATTTAAGATTATCGAAGCAAGCGAAATATACA
    GTTAATTTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCG
    AATTTAATAAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACAT
    ATCGAAAATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATTTAAATGGTACA
    TCTACAAACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAAT
    TTAACTAGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTA
    AAATATGCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTA
    CAGTTGAATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATT
    GAAATTACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATT
    GCAGTAAATGGCACATCAACACCAATTTTATCAGATTTAAAATTTACAGGTGACCCACGT
    GTAGGCTACAAAGATATCTCTAAAAAAGTTAAATCAGTATTGAAGCATGATAGAGGTATC
    GGGGAACGTGAATTAAAATATGCAAAAAAAGCAACTTACACAGTACATTTTAAAAATGGA
    ACGAAAAAAGTGATTAACATAAATTCAAATATTAGCCAACTGAATCTGCTTTATGTTCAA
    GATATTAAAAAGATAGATATTGATGTTAAAACAGGAACTAAAGCGAAAGCGGATAGCTAT
    GTACCATATACAATTGCAGTAAATGGCACATCAACACCAATTTTATCAAAACTTAAAATT
    TCGAATAAACAATTAATTAGTTACAAATATTTAAATGACAAAGTGAAATCTGTATTAAAA
    AGTGAAAGAGGCATCAGTGATCTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTA
    TATTTCAAAAATGGAAAGAAACAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAAT
    TTATTTAGTGCCAAAGATATTAAAAAGATTGATATTGATGTAAAACAATACACTAAATCA
    AAAAAAAATAAATAA
    60
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    840
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    1560
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    1680
    1740
    1755

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS10460 [old locus tag: SACOL2002 ]
  • symbol: SACOL_RS10460
  • description: MAP domain-containing protein
  • length: 584
  • theoretical pI: 10.5258
  • theoretical MW: 65573.8
  • GRAVY: -0.581678

Function[edit | edit source]

  • TIGRFAM:
    modification target Cys-rich peptide, AC3_0185 family (TIGR04118; HMM-score: 13.7)
  • TheSEED: see SACOL2002
  • PFAM:
    Ant-toxin_C (CL0386) MAP; MAP domain (PF03642; HMM-score: 586)
    and 9 more
    no clan defined DUF3553; Protein of unknown function (DUF3553) (PF12073; HMM-score: 21.8)
    Tudor (CL0049) LBR_tudor; Lamin-B receptor of TUDOR domain (PF09465; HMM-score: 20.6)
    no clan defined DUF4333; Domain of unknown function (DUF4333) (PF14230; HMM-score: 17.9)
    GAPES3; Gammaproteobacterial periplasmic sensor domain (PF17154; HMM-score: 13.6)
    Trefoil (CL0066) DUF569; Domain of unknown function (DUF569) (PF04601; HMM-score: 12.8)
    no clan defined Ribosomal_S21e; Ribosomal protein S21e (PF01249; HMM-score: 9.2)
    Cupin (CL0029) VIT_2; Vault protein inter-alpha-trypsin domain (PF13757; HMM-score: 8.9)
    Homing_endonuc (CL0324) Hom_end; Homing endonuclease (PF05204; HMM-score: 8)
    Sm-like (CL0527) LSM14; Scd6-like Sm domain (PF12701; HMM-score: 7.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.8373
    • TAT(Tat/SPI): 0.003408
    • LIPO(Sec/SPII): 0.153715
    • Cleavage Site: CS pos: 30-31. ASA-AA. Pr: 0.5354
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHHGYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRLSKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANYQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSDLKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSNISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKNK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL2002
  • protein localization: see SACOL2002
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]