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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2002 [new locus tag: SACOL_RS10460 ]
  • pan locus tag?: SAUPAN005009000
  • symbol: map
  • pan gene symbol?: eap/map
  • synonym:
  • product: map protein, programmed frameshift

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2002 [new locus tag: SACOL_RS10460 ]
  • symbol: map
  • product: map protein, programmed frameshift
  • replicon: chromosome
  • strand: -
  • coordinates: join(2061282..2061587,2061589..2063337)
  • length: 2055
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAAATTTAAGTCATTGATTACAACAACTTTAGCATTAGGTGTTTTAGCATCAACAGGT
    GCAAACTTTAATAATAATGAAGCGTCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
    TCGTTACACCATGGATATTCTAAAGTCCATGTTCCATATGCAATCACTGTGAACGGTACA
    AGCCAAAATATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
    TTAGAGGATAGAGTTAAATCAGTTTTAAAATCAGACAGAGGTATTAGTGATATAGATTTA
    AGACTATCGAAGCAAGCGAAATATACTGTTTACTTTAAAAATGGAACAAAGAAAGTTATC
    GATTTGAAAGCAGGTATTTACACAGCTGATTTAATTAATACAAGTGAAATTAAAGCTATT
    AATATTAACGTAGATACTAAAAAGCAAGTTGAAGATAAAAAGAAAGATAAAGCAAATTAC
    CAAGTTCCATACACAATCACTGTGAACGGTACAAGCCAAAATATTTTATCAAACTTAACA
    TTTAATAAGAATCAAAATATTAGTTACAAAGATTTAGAGGATAAAGTTAAATCAGTTTTA
    GAATCAAATAGAGGTATTACCGATGTTGATTTAAGATTATCGAAGCAAGCGAAATATACA
    GTTAATTTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCG
    AATTTAATAAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACAT
    ATCGAAAATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATTTAAATGGTACA
    TCTACAAACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAAT
    TTAACTAGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTA
    AAATATGCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTA
    CAGTTGAATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATT
    GAAATTACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATT
    GCAGTAAATGGCACATCAACACCAATTTTATCAGATTTAAAATTTACAGGTGACCCACGT
    GTAGGCTACAAAGATATCTCTAAAAAAGTTAAATCAGTATTGAAGCATGATAGAGGTATC
    GGGGAACGTGAATTAAAATATGCAAAAAAAGCAACTTACACAGTACATTTTAAAAATGGA
    ACGAAAAAAGTGATTAACATAAATTCAAATATTAGCCAACTGAATCTGCTTTATGTTCAA
    GATATTAAAAAGATAGATATTGATGTTAAAACAGGAACTAAAGCGAAAGCGGATAGCTAT
    GTACCATATACAATTGCAGTAAATGGCACATCAACACCAATTTTATCAAAACTTAAAATT
    TCGAATAAACAATTAATTAGTTACAAATATTTAAATGACAAAGTGAAATCTGTATTAAAA
    AGTGAAAGAGGCATCAGTGATCTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTA
    TATTTCAAAAATGGAAAGAAACAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAAT
    TTATTTAGTGCCAAAGATATTAAAAAGATTGATATTGATGTAAAACAATACACTAAATCA
    AAAAAAAATAATAAATCTAATAATGTGAAATTCCCAGTAACAATAAATAAATTTGAAAAC
    ATAGTTTCAAATGAATTTGTGTTCTATAATGCAAGCAAAATTACAATTAATGATTTAAGT
    ATAAAACTTAAATCAGCAATGGCAAATGATCAAGGGATAACTAAACATGACATAGGACTT
    GCTGAACGCGCAGTGTATAAAGTGTATTTTAAAAATGGTTCGTCAAAATATGTAGACTTA
    AAAACTGAGTATAAAGATGAAAGAGTATTTAAAGCAACTGACATTAAAAAGGTAGATATT
    GAACTTAAATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2055

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2002 [new locus tag: SACOL_RS10460 ]
  • symbol: Map
  • description: map protein, programmed frameshift
  • length: 684
  • theoretical pI: 10.458
  • theoretical MW: 77058.8
  • GRAVY: -0.560234

⊟Function[edit | edit source]

  • TIGRFAM:
    modification target Cys-rich peptide, AC3_0185 family (TIGR04118; HMM-score: 13.5)
  • TheSEED  :
    • Extracellular adherence protein of broad specificity Eap/Map
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Extracellular adherence protein of broad specificity Eap/Map
  • PFAM:
    Ant-toxin_C (CL0386) MAP; MAP domain (PF03642; HMM-score: 684.4)
    and 7 more
    no clan defined DUF3553; Protein of unknown function (DUF3553) (PF12073; HMM-score: 20.5)
    Tudor (CL0049) LBR_tudor; Lamin-B receptor of TUDOR domain (PF09465; HMM-score: 19.6)
    no clan defined DUF4333; Domain of unknown function (DUF4333) (PF14230; HMM-score: 16.9)
    GAPES3; Gammaproteobacterial periplasmic sensor domain (PF17154; HMM-score: 13.1)
    Trefoil (CL0066) DUF569; Domain of unknown function (DUF569) (PF04601; HMM-score: 10.4)
    no clan defined Ribosomal_S21e; Ribosomal protein S21e (PF01249; HMM-score: 8)
    Homing_endonuc (CL0324) Hom_end; Homing endonuclease (PF05204; HMM-score: 7.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NEASAAAK
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.8373
    • TAT(Tat/SPI): 0.003408
    • LIPO(Sec/SPII): 0.153715
    • Cleavage Site: CS pos: 30-31. ASA-AA. Pr: 0.5354
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHHGYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRLSKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANYQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSDLKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSNISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKNNKSNNVKFPVTINKFENIVSNEFVFYNASKITINDLSIKLKSAMANDQGITKHDIGLAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cell wall associated [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 15.2 h [4]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

S J Projan, J Kornblum, B Kreiswirth, S L Moghazeh, W Eisner, R P Novick
Nucleotide sequence: the beta-hemolysin gene of Staphylococcus aureus.
Nucleic Acids Res: 1989, 17(8);3305
[PubMed:2726469] [WorldCat.org] [DOI] (P p)

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