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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1917
  • pan locus tag?: SAUPAN005009000
  • symbol: map
  • pan gene symbol?: eap/map
  • synonym:
  • product:

mapW: major histocompatibility complex II analog immune evasion protein, programmed frameshift

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: pseudogene
  • locus tag: SAUSA300_1917
  • symbol: map
  • product:
  • replicon: chromosome
  • strand: -
  • coordinates: 2082045..2084100
  • length: 2056
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAAATTTAAGTCATTGATTACAACAACTTTAGCATTAGGTGTTTTAGCATCAACAGGT
    GCAAACTTTAATAATAATGAAGCGTCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
    TCGTTACACCATGGATATTCTAAAGTCCATGTTCCATATGCAATCACTGTGAACGGTACA
    AGCCAAAATATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
    TTAGAGGATAGAGTTAAATCAGTTTTAAAATCAGACAGAGGTATTAGTGATATAGATTTA
    AGACTATCGAAGCAAGCGAAATATACTGTTTACTTTAAAAATGGAACAAAGAAAGTTATC
    GATTTGAAAGCAGGTATTTACACAGCTGATTTAATTAATACAAGTGAAATTAAAGCTATT
    AATATTAACGTAGATACTAAAAAGCAAGTTGAAGATAAAAAGAAAGATAAAGCAAATTAC
    CAAGTTCCATACACAATCACTGTGAACGGTACAAGCCAAAATATTTTATCAAACTTAACA
    TTTAATAAGAATCAAAATATTAGTTACAAAGATTTAGAGGATAAAGTTAAATCAGTTTTA
    GAATCAAATAGAGGTATTACCGATGTTGATTTAAGATTATCGAAGCAAGCGAAATATACA
    GTTAATTTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCG
    AATTTAATAAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACAT
    ATCGAAAATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATTTAAATGGTACA
    TCTACAAACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAAT
    TTAACTAGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTA
    AAATATGCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTA
    CAGTTGAATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATT
    GAAATTACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATT
    GCAGTAAATGGCACATCAACACCAATTTTATCAGATTTAAAATTTACAGGTGACCCACGT
    GTAGGCTACAAAGATATCTCTAAAAAAGTTAAATCAGTATTGAAGCATGATAGAGGTATC
    GGGGAACGTGAATTAAAATATGCAAAAAAAGCAACTTACACAGTACATTTTAAAAATGGA
    ACGAAAAAAGTGATTAACATAAATTCAAATATTAGCCAACTGAATCTGCTTTATGTTCAA
    GATATTAAAAAGATAGATATTGATGTTAAAACAGGAACTAAAGCGAAAGCGGATAGCTAT
    GTACCATATACAATTGCAGTAAATGGCACATCAACACCAATTTTATCAAAACTTAAAATT
    TCGAATAAACAATTAATTAGTTACAAATATTTAAATGACAAAGTGAAATCTGTATTAAAA
    AGTGAAAGAGGCATCAGTGATCTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTA
    TATTTCAAAAATGGAAAGAAACAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAAT
    TTATTTAGTGCCAAAGATATTAAAAAGATTGATATTGATGTAAAACAATACACTAAATCA
    AAAAAAAATAAATAAATCTAATAATGTGAAATTCCCAGTAACAATAAATAAATTTGAAAA
    CATAGTTTCAAATGAATTTGTGTTCTATAATGCAAGCAAAATTACAATTAATGATTTAAG
    TATAAAACTTAAATCAGCAATGGCAAATGATCAAGGGATAACTAAACATGACATAGGACT
    TGCTGAACGCGCAGTGTATAAAGTGTATTTTAAAAATGGTTCGTCAAAATATGTAGACTT
    AAAAACTGAGTATAAAGATGAAAGAGTATTTAAAGCAACTGACATTAAAAAGGTAGATAT
    TGAACTTAAATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2056

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1917
  • symbol: Map
  • description:
  • length:
  • theoretical pI:
  • theoretical MW:
  • GRAVY:

⊟Function[edit | edit source]

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb:
  • LocateP:
  • SignalP:
  • predicted transmembrane helices (TMHMM):

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; compare RegPrecise for N315  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

There appear to be two alleles at this locus. One, the Map/Eap protein is exemplified by strain Newman; it is the original full-length eap gene. In contrast is the MapW protein. The mapW allele found in FPR3757, N315, Mu3, MW2 and COL is shorter with a poly-A region resulting in frame-slippage and a premature stop codon. Confusing the issue, characterization of the "Eap" protein and it's neutrophil protease, complement, extracellular matrix and eukaryotic cell surface receptor binding, appears to have used the shorter version encoded by mapW from Mu50. Therefore, although the gene nomenclature can use "eap" or "map" for the full-length protein and "mapW" for the shorter allele, the proper nomenclature for the encoded protein is unclear.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Qian Liu, Won-Sik Yeo, Taeok Bae
    The SaeRS Two-Component System of  Staphylococcus aureus.
    Genes (Basel): 2016, 7(10);
    [PubMed:27706107] [WorldCat.org] [DOI] (P e)

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Lawrence Y Lee, Yuko J Miyamoto, Bradley W McIntyre, Magnus Höök, Kirk W McCrea, Damien McDevitt, Eric L Brown
    The Staphylococcus aureus Map protein is an immunomodulator that interferes with T cell-mediated responses.
    J Clin Invest: 2002, 110(10);1461-71
    [PubMed:12438444] [WorldCat.org] [DOI] (P p)    Angus Buckling, James Neilson, Jodi Lindsay, Richard ffrench-Constant, Mark Enright, Nicholas Day, Ruth C Massey
    Clonal distribution and phase-variable expression of a major histocompatibility complex analogue protein in Staphylococcus aureus.
    J Bacteriol: 2005, 187(8);2917-9
    [PubMed:15805539] [WorldCat.org] [DOI] (P p)    Niamh Harraghy, Muzaffar Hussain, Axana Haggar, Triantafyllos Chavakis, Bhanu Sinha, Mathias Herrmann, Jan-Ingmar Flock
    The adhesive and immunomodulating properties of the multifunctional Staphylococcus aureus protein Eap.
    Microbiology (Reading): 2003, 149(Pt 10);2701-2707
    [PubMed:14523103] [WorldCat.org] [DOI] (P p)    Jordan L Woehl, Kasra X Ramyar, Benjamin B Katz, John K Walker, Brian V Geisbrecht
    The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein.
    Protein Sci: 2017, 26(8);1595-1608
    [PubMed:28512867] [WorldCat.org] [DOI] (I p)
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