From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11220 [old locus tag: SACOL2143 ]
  • pan locus tag?: SAUPAN005471000
  • symbol: SACOL_RS11220
  • pan gene symbol?: —
  • synonym:
  • product: HAD family phosphatase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS11220 [old locus tag: SACOL2143 ]
  • symbol: SACOL_RS11220
  • product: HAD family phosphatase
  • replicon: chromosome
  • strand: -
  • coordinates: 2204388..2205245
  • length: 858
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2204388..2205245) NCBI
  • BioCyc: SACOL_RS11220 BioCyc
  • MicrobesOnline: see SACOL2143

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATAGATAACGTAAAAGCAATATTTTTGGACATGGATGGAACAATTTTACATGAAAATAAT
    CAAGCATCAACGTATACGAAAGATGTCATTAATCAATTGAGAGAGAAAGGATATAAAGTA
    TTTTTGGCAACAGGACGTTCGCATTCTGAAATACATCAACTTGTACCTCAAGATTTTGCG
    GTTAATGGCATCATTAGTTCAAATGGAACAATTGGAGAAGTAGATGGAGAAATTATCTTC
    AAGCATGGTTTATCATTGGCTCAAGTGCAACAAATTACTAATTTAGCTAAGCGCCAACAA
    ATTTATTATGAGGTATTTCCTTTTGAAGGTAATAGAGTTTCTTTAAAAGAAGATGAAACA
    TGGATGCGAGATATGATTCGTAGTCAAGATCCTATTAATGGCGTAAGTCATAGTGAATGG
    TCTTCAAGACAAGATGCGCTTGCTGGTAAGATAGATTGGGTAACTAAGTTTCCTGAAGGT
    GAATATTCAAAAATTTATCTATTCAGTTCTAATTTAGAAAAAATAACAGCATTTAGATAT
    GAATTAAAGCAAAATCATGTGCAACTACAGATTAGTGTTTCAAATTCATCAAGATTTAAT
    GCGGAAACAATGGCTTATCAAACTGATAAAGGTACAGGCATTAAAGAAATGATTGCACAT
    TTTGGTATTCATCAAGAAGAAACGTTAGTTATTGGAGATAGCGACAATGATAGAGCAATG
    TTTGAATTTGGTCATTATACAGTTGCTATGAAAAATGCACGCCCTGAAATCCAAGCATTA
    ACTTCAGATGTAACGGCATACACGAATGAAGAGGATGGCGCAGCAAAATATTTAGCAGAG
    CATTTTTTAGCTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    858

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS11220 [old locus tag: SACOL2143 ]
  • symbol: SACOL_RS11220
  • description: HAD family phosphatase
  • length: 285
  • theoretical pI: 4.96362
  • theoretical MW: 32345.9
  • GRAVY: -0.480702

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 169)
    and 19 more
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 87.9)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 87.2)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 83.8)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 49.4)
    mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 46.8)
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 37.7)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 36.5)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 31.7)
    Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 30.3)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 27.2)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 26.1)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 21.4)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 19.1)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 18.7)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 18.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 18.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 18.3)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 13.4)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 12.6)
  • TheSEED: see SACOL2143
  • PFAM:
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 178)
    and 9 more
    S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 56.2)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 38.3)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 22)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 21.1)
    Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 20.1)
    Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 14.8)
    PH (CL0266) UCH_N; N-terminal of ubiquitin carboxyl-terminal hydrolase 37 (PF16674; HMM-score: 14.1)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14)
    Cache (CL0165) sCache_like; Single Cache-like (PF16736; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00549
    • TAT(Tat/SPI): 0.00027
    • LIPO(Sec/SPII): 0.000604
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLATGRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIYYEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEGEYSKIYLFSSNLEKITAFRYELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMIAHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAKYLAEHFLAE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS11220&oldid=101096"