From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12170 [old locus tag: SACOL2314 ]
  • pan locus tag?: SAUPAN005801000
  • symbol: SACOL_RS12170
  • pan gene symbol?: —
  • synonym:
  • product: sodium transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS12170 [old locus tag: SACOL2314 ]
  • symbol: SACOL_RS12170
  • product: sodium transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2374307..2375224
  • length: 918
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2374307..2375224) NCBI
  • BioCyc: SACOL_RS12170 BioCyc
  • MicrobesOnline: see SACOL2314

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTTCAAAAATTGAGTATATTTGCCACGAAGAGTTTTTTGGTATGGATGTTAGTAGCA
    GCTGTTATTGGATTTATTTTCCCACAACATGTTGCGACATTAGGTAAATGGGTACCTTAT
    TTACTTGGTATTGTTATGTTAGGTATGGGATTAACAATTACACCTAATGATTTCAAAATG
    GTCTTTAAAGCACCTAGAGCAGTAATTATTGGTGTCTGTCTACAATTCAGTATTATGCCC
    ACATTAGCATTTATAATTGCAAAGTCTTTTCATTTACCACCTGATATTGCTGTTGGCGTA
    ATATTAGTTGGATGTTGTCCGGGTGGGACATCAAGTAATGTAATGAGTTATTTAGCCAAA
    GCTAACGTAGCACTTTCTGTTTCTATTACGACGGTCTCTACGTTGCTAGCGCCATTCGTT
    ACACCTGCGTTAATATATCTATTTGCAAATGAATGGTTGGAAGTATCTTTCGTGAGTATG
    TTGTGGTCAGTTGTTCAAGTTGTATTAATTCCAATTGCTTTAGGTATTGTTTTGCAAATT
    ATTAATCGTAAAATTGCTGAAAAAGCTTCTACAGCTTTGCCAATTATATCAGTTGTTGCT
    ATTTCATTAATTTTAGCAATAGTTGTAGGTGGCAGTAAGCACCAAATCTTAACTACAGGA
    TTATTAATATTTTTAGTAGTTATTTTACATAACGTATTAGGGTATACGATTGGATATTGG
    TTAGCTCGTCTTTTAAAATTAGATCGACAAGATCAAAAAGCAGTCAGTATTGAAGTTGGA
    ATGCAGAACTCTGGTTTAGCTGTGTCATTAGCAGCATTGCATTTTAATCCAATTGCAGCA
    GTACCAGGCGCAGTGTTTAGTTTCATTCATAATATAACAGGGCCTATTTTAGCAAAGTAT
    TGGTCAAAAAAGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS12170 [old locus tag: SACOL2314 ]
  • symbol: SACOL_RS12170
  • description: sodium transporter
  • length: 305
  • theoretical pI: 10.2753
  • theoretical MW: 32851.6
  • GRAVY: 1.06951

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids bile acid transporter (TIGR00841; HMM-score: 289)
    and 1 more
    membrane protein AbrB duplication (TIGR03082; HMM-score: 11.3)
  • TheSEED: see SACOL2314
  • PFAM:
    CPA_AT (CL0064) SBF; Sodium Bile acid symporter family (PF01758; HMM-score: 155.2)
    and 3 more
    SBF_like; SBF-like CPA transporter family (DUF4137) (PF13593; HMM-score: 77.2)
    Mem_trans; Membrane transport protein (PF03547; HMM-score: 10.4)
    no clan defined DUF3464; Protein of unknown function (DUF3464) (PF11947; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025516
    • TAT(Tat/SPI): 0.001144
    • LIPO(Sec/SPII): 0.015437
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGIVMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVILVGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSMLWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILTTGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFNPIAAVPGAVFSFIHNITGPILAKYWSKKL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: see SACOL2314
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS12170&oldid=101005"