PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS12905 [old locus tag: SACOL2460 ]
• pan locus tag^?: SAUPAN006008000
• symbol: SACOL_RS12905
• pan gene symbol^?: —
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12905 [old locus tag: SACOL2460 ]
• symbol: SACOL_RS12905
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2518676..2519854
• length: 1179
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2518676..2519854) NCBI
• BioCyc: SACOL_RS12905 BioCyc
• MicrobesOnline: see SACOL2460

⊟Phenotype

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⊟DNA sequence

• 1
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  481
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  1021
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  1141
  
  ATGCGACTATTTACATTCATTTTAAGTATTTTTATCGTAGGAATGGTTGAAATGATGGTT
  GCAGGAATTATGAACTTGATGAGTCAGGACTTACATGTATCAGAAGCTGTCGTTGGTCAA
  TTAGTGACAATGTACGCTTTAACATTTGCGATATGTGGACCTATTCTGGTTAAATTAACG
  AACCGTTTTTCATCAAGGCCTGTATTATTATGGACATTACTTATATTTATCATTGGTAAT
  GGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATTATCTCATCT
  GCAGCAGCAGCACTAATTATCGTAAAAGTATTAGCTATTACAGCGATGTTATCAGCACCT
  AAAAATCGTGGTAAAATGATTGGACTTGTCTATACAGGGTTTAGTGGTGCTAATGTTTTT
  GGTGTACCAATTGGAACGGTTATCGGCGATTTAGTAGGTTGGCGCTATACATTTCTATTC
  TTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGATGATCTATTTACCGAAGGATCAG
  GAAATACAACGAGGCCCTGTGAATCATGAGACACCATCTCATGAAAATCATGTTACTTCG
  AAAATATTAAGACCTGCTGAAGTAGCAAAATATTTAATTATTACGTTTTTAGTATTGATT
  GCAAACTCAGTGACATTCGTCTTTATAAATCCACTTATTTTATCTAATGGTCATGATATG
  TCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGAACATCATTA
  GGTGGTATATTCTCCGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCTGTTTCTATT
  TTTATCGTCATGATGTTACTTATGAATTTAATCTTACCTGGTTCAGGTCTATTGTTAGCA
  GGACTATTTATTTGGAATATCATGCAATGGAGTACTAATCCAGCAGTGCAAAGCGGTGTG
  ATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCTAGTTTAAAC
  GCTGGTATTGGTGTTGGAGGCATTATTGGAGGCTTGGTCATGACACATGTTTCTGTTCAA
  GCTATCACATATACGAGTGCCATCATTGGCGCATTAGGATTAATCGTTGTTTTCACATTG
  AAAAATAATCATTATGCTAAAACATTTAAATCATCATAA
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  1179
  

⊟Protein

⊟General

• locus tag: SACOL_RS12905 [old locus tag: SACOL2460 ]
• symbol: SACOL_RS12905
• description: MFS transporter
• length: 392
• theoretical pI: 10.1636
• theoretical MW: 42243.4
• GRAVY: 0.959184

⊟Function

• TIGRFAM:
  multidrug resistance protein (TIGR00880; HMM-score: 96.1)
  and 10 more
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 72.3)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.3)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 65.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 48.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 42)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 36.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 25.2)
  Metabolism Transport and binding proteins Other cyanate transporter (TIGR00896; HMM-score: 24.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 22.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 8.8)
• TheSEED: see SACOL2460
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 198.7)
  and 2 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 27.4)
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.196203
  • TAT(Tat/SPI): 0.000913
  • LIPO(Sec/SPII): 0.046493
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: see SACOL2460
• RefSeq: WP_001802848 NCBI
• UniProt: see SACOL2460

⊟Protein sequence

• MRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL2460
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications