PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS12980 [old locus tag: SACOL2476 ]
• pan locus tag^?: SAUPAN006027000
• symbol: SACOL_RS12980
• pan gene symbol^?: cntA
• synonym:
• product: nickel ABC transporter, nickel/metallophore periplasmic binding protein

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12980 [old locus tag: SACOL2476 ]
• symbol: SACOL_RS12980
• product: nickel ABC transporter, nickel/metallophore periplasmic binding protein
• replicon: chromosome
• strand: -
• coordinates: 2532192..2533790
• length: 1599
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2532192..2533790) NCBI
• BioCyc: SACOL_RS12980 BioCyc
• MicrobesOnline: see SACOL2476

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  
  ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTACATCAGGGCTAATTTTAACTGGT
  TGTGGCGGTAATAAAGGTTTAGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACG
  GTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTGGATCAATGTCTGCTGAAAGT
  ATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGTATTAAGCCTTTACTAGCTAAA
  AAGTGGGATGTGTCTGAAGATGGGAAGACATACACGTTCCATTTGAGAGATGACGTTAAA
  TTCCATGATGGTACGCCATTTGATGCTGACGCAGTTAAGAAAAATATTGACGCAGTTCAA
  GAAAACAAAAAATTGCATTCTTGGTTAAAGATTTCGACATTAATTGACAATGTTAAAGTT
  AAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
  TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACA
  AAAGATGGCGTTAAAAAGTTCGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAA
  GATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGGGCGAAAAGTCTAAACTTAAC
  AAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
  GAAACGAACTTTGCCTTCACAGATGATAGAGGTACAGATAGCTTAGACAAAGACTCTTTA
  AAACAATTGAAAGATACAGGTGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAA
  ATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTGAGTGACAAAACAGTCAGACAA
  GCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
  AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACA
  CGTAAGTATGACCTTAAAAAAGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGT
  AAAGACAGCGATGTTCGTCAAAAAGATGGTAAAAACCTTGAAATGGCAATGTACTATGAC
  AAAGGTTCTTCAAGTCAAAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
  GGTATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCT
  GGTGATTATGACTTAATGTTCAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACT
  ATTGCAGCATTTAAAGCGAAAAATGGTTATGAAAGTGCAACATCAGGCATTGAGAACAAA
  GATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
  GACGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCA
  CACGGTAGTATGACAGTTGTTGCACCAAAAGATTTAGAAAAAGTATCATTCACACAATCA
  CAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1599
  

⊟Protein

⊟General

• locus tag: SACOL_RS12980 [old locus tag: SACOL2476 ]
• symbol: SACOL_RS12980
• description: nickel ABC transporter, nickel/metallophore periplasmic binding protein
• length: 532
• theoretical pI: 9.23365
• theoretical MW: 60048.7
• GRAVY: -0.814662

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 787.6)
  and 1 more
  Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 115.2)
• TheSEED: see SACOL2476
• PFAM:
  PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 278.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 9.21
  • Extracellular Score: 0.78
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.442
  • Cell wall & surface Score: 0.0764
  • Extracellular Score: 0.4814
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.000471
  • TAT(Tat/SPI): 0.000107
  • LIPO(Sec/SPII): 0.999036
  • Cleavage Site: CS pos: 20-21. LTG-CG. Pr: 0.9998
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447151839 NCBI
• RefSeq: WP_001229095 NCBI
• UniProt: see SACOL2476

⊟Additional information (user-provided)

⊟Protein sequence

• MRKLTKMSAMLLTSGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

⊟Experimental data

• experimentally validated: see SACOL2476
• protein localization: see SACOL2476
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications