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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2255 [new locus tag: SA_RS12950 ]
  • pan locus tag?: SAUPAN006027000
  • symbol: opp-1A
  • pan gene symbol?: cntA
  • synonym:
  • product: oligopeptide transporter substrate binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2255 [new locus tag: SA_RS12950 ]
  • symbol: opp-1A
  • product: oligopeptide transporter substrate binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2531455..2533053
  • length: 1599
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTGCATCAGGGCTAATTTTAACTGGT
    TGTGGCGGTAATAAAGGTTTAGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACG
    GTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTGGATCAATGTCTGCTGAAAGT
    ATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGTATTAAGCCTTTACTAGCTAAA
    AAATGGGATGTGTCTGAAGATGGGAAGACTTATACATTCCATTTAAGAGATGATGTGAAA
    TTCCATGATGGAACAACATTTGATGCTGACGCAGTTAAGAAAAATATTGATGCGGTTCAA
    CAAAATAAAAAATTGCATTCTTGGTTAAAGATTTCAACATTAATTGACAATGTTAAAGTT
    AAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
    TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACA
    AAAGATGGCGTTAAAAAGTTCGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAA
    GATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGGGCGAAAAGTCTAAACTTAAC
    AAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
    GAAACGAACTTTGCCTTCACAGATGATAGAGGTACGGATAGCTTAGACAAAGACTCTTTA
    AAACAATTGAAAGATACAGGTGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAA
    ATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTCAGTGACAAAACAGTCAGACAA
    GCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
    AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACA
    CGTAAGTATGACCTTAAAAAAGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGT
    AAAGACAGTGATGTTCGTCAAAAAGATGGTAAAAACCTTGAAATGGCAATGTACTATGAC
    AAAGGTTCTTCAAGTCAAAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
    GGTATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCT
    GGTGATTATGACTTAATGTTCAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACT
    ATTGCAGCATTTAAAGCGAAAAATGGTTATGAAAGTGCAACATCAGGCATTGAGAACAAA
    GATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
    GACGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCA
    CACGGTAGTATGACAGTTGTTGCGCCGAAAGATTTAGAAAAAGTATCATTCACACAATCA
    CAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1599

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2255 [new locus tag: SA_RS12950 ]
  • symbol: Opp-1A
  • description: oligopeptide transporter substrate binding protein
  • length: 532
  • theoretical pI: 9.32649
  • theoretical MW: 60021.7
  • GRAVY: -0.808271

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 784.7)
    and 1 more
    Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 112.2)
  • TheSEED  :
    • Staphylopine-metal ABC transporter, substrate-binding protein CntA
    Membrane Transport ABC transporters ABC transporter dipeptide (TC 3.A.1.5.2)  Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2)
    and 1 more
    Motility and Chemotaxis Motility and Chemotaxis - no subcategory Bacterial Chemotaxis  Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2)
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 262.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.21
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LILTGCG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000447
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.999166
    • Cleavage Site: CS pos: 20-21. LTG-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol Microbiol: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)

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