PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2250 [new locus tag: SA_RS12925 ]
• pan locus tag^?: SAUPAN006022000
• symbol: SA2250
• pan gene symbol^?: cntE
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2250 [new locus tag: SA_RS12925 ]
• symbol: SA2250
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2526882..2528075
• length: 1194
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125178 NCBI
• RefSeq: NP_375574 NCBI
• BioCyc: see SA_RS12925
• MicrobesOnline: 104600 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1141
  
  ATGAAAGGTGCAATGGCTTGGCCCTTTTTGAGATTATATATATTAACATTGATGTTCTTT
  AGTGCCAATGCAATCTTAAACGTATTTATACCGTTACGAGGGCATGATTTAGGCGCAACG
  AATACGGTTATCGGTATCGTTATGGGGGCATACATGTTAACAGCAATGGTGTTTCGACCA
  TGGGCAGGACAAATTATTGCTCGTGTCGGTCCCATTAAAGTTTTAAGAATTATTTTGATC
  ATCAATGCCATAGCTTTAATTATTTATGGTTTTACTGGATTAGAAGGTTATTTCGTAGCA
  CGTGTTATGCAAGGTGTGTGTACGGCATTCTTTTCTATGTCTTTACAGTTAGGTATTATT
  GATGCATTACCAGAGGAACATCGTTCTGAAGGTGTATCATTGTACTCGCTATTTTCAACG
  ATTCCAAACTTAATTGGACCATTAGTTGCCGTAGGTATTTGGAATGCAAATAATATTTCA
  CTATTTGCAATTGTCATTATCTTTATCGCATTAACAACAACATTCTTTGGCTATCGCGTG
  ACCTTTGCTGAACAGGAACCCGATACGTCAGATAAGATTGAAAAAATGCCGTTTAACGCT
  GTAACTGTTTTTGCGCAATTTTTCAAAAATAAAGAGTTGTTGAACAGTGGTATTATCATG
  ATTGTTGCATCGATTGTATTTGGTGCAGTTAGTACATTTGTACCGTTATACACAGTGAGT
  TTAGGATTCGCGAATGCGGGAATCTTTTTGACAATACAGGCCATCGCAGTTGTTGCGGCA
  AGATTTTACTTAAGGAAATACATTCCGTCAGATGGTATGTGGCATCCTAAATATATGGTA
  TCTGTACTATCATTATTAGTAATCGCGTCATTTGTAGTGGCATTTGGTCCGCAAGTAGGT
  GCAATTATTTTCTATGGTAGTGCGATATTAATAGGAATGACGCAAGCAATGGTGTACCCA
  ACATTAACATCATACTTAAGCTTCGTCTTACCAAAAGTAGGTCGTAATATGTTGTTAGGT
  TTATTTATTGCCTGTGCAGACTTAGGTATATCGTTAGGTGGCGCATTGATGGGACCTATT
  TCCGATTTAGTAGGATTTAAATGGATGTATCTAATTTGTGGTATGTTAGTCATTGTAATA
  ATGATTATGAGTTTCTTGAAAAAGCCAACACCACGTCCAGCGAGTAGTCTTTAA
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⊟Protein

⊟General

• locus tag: SA2250 [new locus tag: SA_RS12925 ]
• symbol: SA2250
• description: hypothetical protein
• length: 397
• theoretical pI: 9.73958
• theoretical MW: 43294.9
• GRAVY: 0.979849

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 46.1)
  and 5 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 36.8)
  multidrug resistance protein (TIGR00880; HMM-score: 33.5)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.8)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 7.1)
• TheSEED  :
  • Staphylopine exporter, MFS-family
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 135.9)
  and 2 more
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 29.8)
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.127089
  • TAT(Tat/SPI): 0.001388
  • LIPO(Sec/SPII): 0.084986
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15928041 NCBI
• RefSeq: NP_375574 NCBI
• UniProt: A0A0H3JVZ8 UniProt

⊟Protein sequence

• MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVIGIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARVMQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNISLFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGIIMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHPKYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVGRNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPRPASSL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2250 < opp-1F < opp-1D < opp-1C < opp-1B < opp-1A < SA2256 < SA2257

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications