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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
- pan locus tag?: SAUPAN006022000
- symbol: SACOL2471
- pan gene symbol?: cntE
- synonym:
- product: transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
- symbol: SACOL2471
- product: transporter
- replicon: chromosome
- strand: -
- coordinates: 2527619..2528812
- length: 1194
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237241 NCBI
- RefSeq: YP_187268 NCBI
- BioCyc:
- MicrobesOnline: 913945 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGAAAGGTGCAATGGCTTGGCCCTTTTTGAGATTATATATATTAACATTGATGTTCTTT
AGTGCCAATGCAATCTTAAACGTGTTTATACCTTTACGAGGGCATGATTTAGGCGCAACG
AATACGGTTATCGGTATCGTTATGGGGGCATACATGTTAACAGCAATGGTATTTCGACCA
TGGGCAGGACAAATTATTGCTCGTGTCGGTCCCATTAAAGTATTAAGATTTATTTTGATT
ATCAATGCCATAGCTTTAATTATTTATGGTTTTACTGGCTTAGAAGGTTATTTCGTAGCA
CGTGTTATGCAAGGTGTGTGTACGGCATTCTTTTCTATGTCTTTACAGCTAGGTATTATT
GATGCATTACCAGAGGAACATCGTTCTGAAGGTGTATCATTGTACTCGCTATTTTCAACG
ATTCCAAACTTAATCGGACCATTAGTTGCCGTAGGTATTTGGAATGCAAATAATATTTCA
CTATTTGCAATTGTCATTATCTTTATCGCATTAACAACAACATTCTTTGGCTATCGCGTG
ACCTTTGCTGAACAGGAACCCGATACGTCAGATAAGATTGAAAAAATGCCGTTTAACGCT
GTAACTGTTTTTGCGCAATTTTTCAAAAATAAAGAGTTGTTGAACAGTGGTATTATCATG
ATTGTTGCATCGATTGTATTTGGTGCAGTTAGTACATTTGTACCGTTATACACAGTGAGT
TTAGGATTCGCGAATGCGGGAATCTTTTTGACAATACAGGCCATCGCAGTTGTTGCGGCA
AGATTTTACTTAAGGAAATACATTCCGTCAGATGGTATGTGGCATCCTAAATATATGGTA
TCTGTACTATCATTATTAGTAATCGCGTCATTTGTAGTGGCATTTGGTCCGCAAGTAGGT
GCAATTATTTTCTATGGTAGTGCGATATTAATAGGAATGACGCAAGCAATGGTGTACCCA
ACATTAACATCATACTTAAGCTTCGTCTTACCAAAAGTAGGTCGTAATATGTTGTTAGGT
TTATTTATTGCCTGTGCAGACTTAGGTATATCGTTAGGTGGCGCATTGATGGGACCTATT
TCCGATTTAGTAGGATTTAAATGGATGTATCTAATTTGTGGTATGTTAGTCATTGTAATA
ATGATTATGAGTTTCTTGAAAAAGCCAACACCACGTCCAGCGAGTAGTCTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
- symbol: SACOL2471
- description: transporter
- length: 397
- theoretical pI: 9.73958
- theoretical MW: 43328.9
- GRAVY: 0.975567
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 44.6)and 5 moreTransport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 35.3)multidrug resistance protein (TIGR00880; HMM-score: 33.7)Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 21.6)Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 21.6)Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 6.5)
- TheSEED :
- Antibiotic resistance protein
- PFAM: MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 135.4)and 2 moreMFS_1_like; MFS_1 like family (PF12832; HMM-score: 28.1)Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.127089
- TAT(Tat/SPI): 0.001388
- LIPO(Sec/SPII): 0.084986
- predicted transmembrane helices (TMHMM): 12
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVIGIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRFILIINAIALIIYGFTGLEGYFVARVMQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNISLFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGIIMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHPKYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVGRNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPRPASSL
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: Integral membrane [1]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e)
⊟Relevant publications[edit | edit source]