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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
  • pan locus tag?: SAUPAN006022000
  • symbol: SACOL2471
  • pan gene symbol?: cntE
  • synonym:
  • product: transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
  • symbol: SACOL2471
  • product: transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2527619..2528812
  • length: 1194
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAGGTGCAATGGCTTGGCCCTTTTTGAGATTATATATATTAACATTGATGTTCTTT
    AGTGCCAATGCAATCTTAAACGTGTTTATACCTTTACGAGGGCATGATTTAGGCGCAACG
    AATACGGTTATCGGTATCGTTATGGGGGCATACATGTTAACAGCAATGGTATTTCGACCA
    TGGGCAGGACAAATTATTGCTCGTGTCGGTCCCATTAAAGTATTAAGATTTATTTTGATT
    ATCAATGCCATAGCTTTAATTATTTATGGTTTTACTGGCTTAGAAGGTTATTTCGTAGCA
    CGTGTTATGCAAGGTGTGTGTACGGCATTCTTTTCTATGTCTTTACAGCTAGGTATTATT
    GATGCATTACCAGAGGAACATCGTTCTGAAGGTGTATCATTGTACTCGCTATTTTCAACG
    ATTCCAAACTTAATCGGACCATTAGTTGCCGTAGGTATTTGGAATGCAAATAATATTTCA
    CTATTTGCAATTGTCATTATCTTTATCGCATTAACAACAACATTCTTTGGCTATCGCGTG
    ACCTTTGCTGAACAGGAACCCGATACGTCAGATAAGATTGAAAAAATGCCGTTTAACGCT
    GTAACTGTTTTTGCGCAATTTTTCAAAAATAAAGAGTTGTTGAACAGTGGTATTATCATG
    ATTGTTGCATCGATTGTATTTGGTGCAGTTAGTACATTTGTACCGTTATACACAGTGAGT
    TTAGGATTCGCGAATGCGGGAATCTTTTTGACAATACAGGCCATCGCAGTTGTTGCGGCA
    AGATTTTACTTAAGGAAATACATTCCGTCAGATGGTATGTGGCATCCTAAATATATGGTA
    TCTGTACTATCATTATTAGTAATCGCGTCATTTGTAGTGGCATTTGGTCCGCAAGTAGGT
    GCAATTATTTTCTATGGTAGTGCGATATTAATAGGAATGACGCAAGCAATGGTGTACCCA
    ACATTAACATCATACTTAAGCTTCGTCTTACCAAAAGTAGGTCGTAATATGTTGTTAGGT
    TTATTTATTGCCTGTGCAGACTTAGGTATATCGTTAGGTGGCGCATTGATGGGACCTATT
    TCCGATTTAGTAGGATTTAAATGGATGTATCTAATTTGTGGTATGTTAGTCATTGTAATA
    ATGATTATGAGTTTCTTGAAAAAGCCAACACCACGTCCAGCGAGTAGTCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2471 [new locus tag: SACOL_RS12955 ]
  • symbol: SACOL2471
  • description: transporter
  • length: 397
  • theoretical pI: 9.73958
  • theoretical MW: 43328.9
  • GRAVY: 0.975567

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 44.6)
    and 5 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 35.3)
    multidrug resistance protein (TIGR00880; HMM-score: 33.7)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 21.6)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 21.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 6.5)
  • TheSEED  :
    • Antibiotic resistance protein
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 135.4)
    and 2 more
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 28.1)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.127089
    • TAT(Tat/SPI): 0.001388
    • LIPO(Sec/SPII): 0.084986
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVIGIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRFILIINAIALIIYGFTGLEGYFVARVMQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNISLFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGIIMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHPKYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVGRNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPRPASSL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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