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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2359 [new locus tag: NWMN_RS13600 ]
  • pan locus tag?: SAUPAN006022000
  • symbol: NWMN_2359
  • pan gene symbol?: cntE
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2359 [new locus tag: NWMN_RS13600 ]
  • symbol: NWMN_2359
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2596115..2597308
  • length: 1194
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAGGTGCAATGGCTTGGCCCTTTTTGAGATTATATATATTAACATTGATGTTCTTT
    AGTGCCAATGCAATCTTAAACGTGTTTATACCTTTACGAGGGCATGATTTAGGCGCAACG
    AATACGGTTATCGGTATCGTTATGGGGGCATACATGTTAACAGCAATGGTATTTCGACCA
    TGGGCAGGACAAATTATTGCTCGTGTCGGTCCCATTAAAGTATTAAGAATTATTTTGATT
    ATCAATGCCATAGCTTTAATTATTTATGGTTTTACTGGCTTAGAAGGTTATTTCGTAGCA
    CGTGTTATGCAAGGTGTGTGTACGGCATTCTTTTCTATGTCTTTACAGCTAGGTATTATT
    GATGCATTACCAGAGGAACATCGTTCTGAAGGTGTATCATTGTACTCGCTATTTTCAACG
    ATTCCAAACTTAATCGGACCATTAGTTGCCGTAGGTATTTGGAATGCAAATAATATTTCA
    CTATTTGCAATTGTCATTATCTTTATCGCATTAACAACAACATTCTTTGGCTATCGCGTG
    ACCTTTGCTGAACAGGAACCCGATACGTCAGATAAGATTGAAAAAATGCCGTTTAACGCT
    GTAACTGTTTTTGCGCAATTTTTCAAAAATAAAGAGTTGTTGAACAGTGGTATTATCATG
    ATTGTTGCATCGATTGTATTTGGTGCAGTTAGTACATTTGTACCGTTATACACAGTGAGT
    TTAGGATTCGCGAATGCGGGAATCTTTTTGACAATACAGGCCATCGCAGTTGTTGCGGCA
    AGATTTTACTTAAGGAAATACATTCCGTCAGATGGTATGTGGCATCCTAAATATATGGTA
    TCTGTACTATCATTATTAGTAATCGCGTCATTTGTAGTGGCATTTGGTCCGCAAGTAGGT
    GCAATTATTTTCTATGGTAGTGCGATATTAATAGGAATGACGCAAGCAATGGTGTACCCA
    ACATTAACATCATACTTAAGCTTCGTCTTACCAAAAGTAGGTCGTAATATGTTGTTAGGT
    TTATTTATTGCCTGTGCAGACTTAGGTATATCGTTAGGTGGCGCATTGATGGGACCTATT
    TCCGATTTAGTAGGATTTAAATGGATGTATCTAATTTGTGGTATGTTAGTCATTGTAATA
    ATGATTATGAGTTTCTTGAAAAAGCCAACACCACGTCCAGCGAGTAGTCTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2359 [new locus tag: NWMN_RS13600 ]
  • symbol: NWMN_2359
  • description: hypothetical protein
  • length: 397
  • theoretical pI: 9.73958
  • theoretical MW: 43294.9
  • GRAVY: 0.979849

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 46.1)
    and 5 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 36.8)
    multidrug resistance protein (TIGR00880; HMM-score: 33.5)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.8)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 7.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 135.9)
    and 2 more
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 29.8)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 18.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.127089
    • TAT(Tat/SPI): 0.001388
    • LIPO(Sec/SPII): 0.084986
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVIGIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARVMQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNISLFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGIIMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHPKYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVGRNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPRPASSL

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]