PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2363 [new locus tag: NWMN_RS13620 ]
• pan locus tag^?: SAUPAN006026000
• symbol: opp1B
• pan gene symbol^?: cntB
• synonym:
• product: oligopeptide transporter putative membrane permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2363 [new locus tag: NWMN_RS13620 ]
• symbol: opp1B
• product: oligopeptide transporter putative membrane permease
• replicon: chromosome
• strand: -
• coordinates: 2599740..2600675
• length: 936
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332220 NCBI
• RefSeq: YP_001333397 NCBI
• BioCyc:
• MicrobesOnline: 3707965 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGTTCAAATTTATCTTAAAACGTATTGCGCTCATGTTTCCATTGATGATTGTAGTAAGT
  TTTATGACATTTCTATTGACGTATATTACAAATGAAAATCCAGCTGTGACAATTTTACAT
  GCACAAGGGACGCCAAATGTAACACCAGAGTTGATTGCAGAAACGAATGAGAAGTACGGT
  TTCAATGATCCATTATTAATTCAATATAAAAATTGGTTACTTGAAGCGATGCAATTTAAT
  TTTGGTACAAGCTACATTACAGGTGACCCAGTTGCTGAACGTATTGGTCCAGCATTTATG
  AATACATTGAAATTAACAATAATTTCAAGTGTTATGGTGATGATTACATCAATTATTTTA
  GGTGTAGTTAGTGCATTAAAAAGAGGAAAGTTCACTGATCGTGCGATACGTTCAGTGGCT
  TTCTTTCTAACTGCATTACCATCATATTGGATAGCTTCAATACTTATTATTTACGTTTCA
  GTGAAGTTAAACATATTGCCGACTTCTGGATTAACAGGTCCAGAAAGTTACATATTGCCA
  GTGATCGTTATTACGATTGCCTATGCTGGTATTTACTTTAGAAATGTTAGACGCTCGATG
  GTGGAACAATTAAATGAAGATTATGTACTTTATTTAAGAGCAAGCGGTGTGAAATCTATC
  ACATTAATGTTGCATGTGTTGCGTAATGCTTTACAAGTTGCGGTATCAATCTTTTGTATG
  TCTATACCAATGATAATGGGTGGACTAGTTGTTATCGAGTATATCTTTGCATGGCCTGGA
  CTAGGTCAATTAAGTTTAAAAGCAATACTTGAACACGATTTTCCAGTCATTCAAGCATAT
  GTATTAATTGTAGCGGTATTATTTATTGTATTTAATACATTAGCAGATATCATTAATGCG
  CTATTAAATCCAAGATTAAGGGAGGGCGCACGATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  936
  

⊟Protein

⊟General

• locus tag: NWMN_2363 [new locus tag: NWMN_RS13620 ]
• symbol: Opp1B
• description: oligopeptide transporter putative membrane permease
• length: 311
• theoretical pI: 9.51657
• theoretical MW: 34780.4
• GRAVY: 0.746302

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 284.4)
  and 1 more
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 11.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 140.8)
  and 1 more
  no clan defined DUF4133; Domain of unknown function (DUF4133) (PF13571; HMM-score: 9.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.461507
  • TAT(Tat/SPI): 0.002052
  • LIPO(Sec/SPII): 0.016595
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 151222575 NCBI
• RefSeq: YP_001333397 NCBI
• UniProt: A0A0H3KAB6 UniProt

⊟Protein sequence

• MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGTPNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVSVKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2359 < opp1F < opp1D < opp1C < opp1B < opp1A

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol Microbiol: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)