From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2254 [new locus tag: SA_RS12945 ]
  • pan locus tag?: SAUPAN006026000
  • symbol: opp-1B
  • pan gene symbol?: cntB
  • synonym:
  • product: oligopeptide transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2254 [new locus tag: SA_RS12945 ]
  • symbol: opp-1B
  • product: oligopeptide transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2530507..2531442
  • length: 936
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTCAAATTTATCTTAAAACGTATTGCGCTCATGTTTCCATTAGTGATTGTAGTAAGT
    TTTATGACATTTCTATTGACGTATATTACAAATGAAAATCCAGCTGTGACAATTTTACAT
    GCACAAGGAACGCCAAATGTAACACCAGAGTTGATTGCAGAAACGAATGAAAAGTACGGT
    TTCAATGATCCATTATTAATTCAATATAAAAACTGGTTACTTGAAGCGATGCAATTTAAT
    TTTGGTACAAGTTACATTACAGGGGATCCTGTTGCTGAACGCATTGGCCCAGCATTTATG
    AACACATTGAAATTAACAATAATTTCAAGTGTTATGGTGATGATTACATCAATTATTTTA
    GGTGTAGTTAGTGCATTAAAAAGAGGAAAGTTCACTGATCGTGCGATACGTTCAGTGGCT
    TTCTTTCTAACTGCATTACCATCATATTGGATAGCTTCAATACTTATTATTTACGTTTCA
    GTGAAGTTAAACATATTGCCGACTTCTGGATTAACAGGCCCAGAAAGTTACATATTGCCA
    GTGATCGTTATTACGATTGCCTATGCTGGTATTTACTTTAGAAATGTTAGACGCTCGATG
    GTGGAACAATTAAATGAAGATTATGTACTTTATTTAAGAGCAAGCGGTGTGAAATCTATC
    ACATTAATGTTGCATGTGTTGCGTAATGCTATACAAGTTGCGGTATCAATCTTTTGTATG
    TCTATACCAATGATAATGGGTGGACTAGTTGTTATCGAGTATATCTTTGCATGGCCTGGA
    CTAGGTCAATTAAGTTTAAAAGCAATACTTGAACACGATTTTCCAGTCATTCAAGCATAT
    GTATTAATTGTAGCGGTATTATTTATTGTATTTAATACATTAGCAGATATCATTAATGCG
    CTATTAAATCCAAGATTAAGGGAGGGCGCACGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2254 [new locus tag: SA_RS12945 ]
  • symbol: Opp-1B
  • description: oligopeptide transporter permease
  • length: 311
  • theoretical pI: 9.51657
  • theoretical MW: 34748.3
  • GRAVY: 0.755949

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 285.4)
    and 1 more
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 10.8)
  • TheSEED  :
    • Staphylopine-metal ABC transporter, permease protein CntB
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 141)
    and 1 more
    no clan defined DUF4133; Domain of unknown function (DUF4133) (PF13571; HMM-score: 9.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.472172
    • TAT(Tat/SPI): 0.001861
    • LIPO(Sec/SPII): 0.014789
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGTPNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNTLKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVSVKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVKSITLMLHVLRNAIQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPVIQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol Microbiol: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA2254&oldid=85945"