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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13535 [old locus tag: SACOL2584 ]
  • pan locus tag?: SAUPAN006235000
  • symbol: SACOL_RS13535
  • pan gene symbol?: isaA
  • synonym:
  • product: transglycosylase IsaA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS13535 [old locus tag: SACOL2584 ]
  • symbol: SACOL_RS13535
  • product: transglycosylase IsaA
  • replicon: chromosome
  • strand: -
  • coordinates: 2648751..2649452
  • length: 702
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2648751..2649452) NCBI
  • BioCyc: SACOL_RS13535 BioCyc
  • MicrobesOnline: see SACOL2584

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAAAGACAATTATGGCATCATCATTAGCAGTGGCATTAGGTGTAACAGGTTACGCA
    GCAGGTACAGGACATCAAGCACACGCTGCTGAAGTAAACGTTGATCAAGCACACTTAGTT
    GACTTAGCGCATAATCACCAAGATCAATTAAATGCAGCTCCAATCAAAGATGGTGCATAT
    GACATCCACTTTGTAAAAGATGGTTTCCAATATAACTTCACTTCAAATGGTACTACATGG
    TCATGGAGCTATGAAGCAGCTAATGGTCAAACTGCTGGTTTCTCAAACGTTGCAGGTGCA
    GACTACACTACTTCATACAACCAAGGTTCAAATGTACAATCAGTAAGCTACAATGCACAA
    TCAAGTAACTCAAACGTTGAAGCTGTTTCAGCTCCAACTTACCATAACTACAGCACTTCA
    ACTACTTCAAGTTCAGTGAGATTAAGCAATGGTAATACTGCAGGTGCTACTGGTTCATCA
    GCAGCTCAAATCATGGCTCAACGTACTGGTGTTTCAGCTTCTACATGGGCTGCAATCATC
    GCTCGTGAATCAAATGGTCAAGTAAATGCTTACAACCCATCAGGTGCTTCAGGTTTATTC
    CAAACTATGCCAGGTTGGGGTCCAACAAACACTGTTGACCAACAAATCAACGCAGCTGTT
    AAAGCATACAAAGCACAAGGTTTAGGTGCTTGGGGATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    702

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS13535 [old locus tag: SACOL2584 ]
  • symbol: SACOL_RS13535
  • description: transglycosylase IsaA
  • length: 233
  • theoretical pI: 6.62819
  • theoretical MW: 24203
  • GRAVY: -0.338197

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.-.-?  ExPASy
  • TIGRFAM:
  • TheSEED: see SACOL2584
  • PFAM:
    Lysozyme (CL0037) SLT; Transglycosylase SLT domain (PF01464; HMM-score: 28.3)
    and 2 more
    Transglycosylas; Transglycosylase-like domain (PF06737; HMM-score: 21.9)
    no clan defined DUF945; Bacterial protein of unknown function (DUF945) (PF06097; HMM-score: 15.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.994875
    • TAT(Tat/SPI): 0.002931
    • LIPO(Sec/SPII): 0.001546
    • Cleavage Site: CS pos: 29-30. AHA-AE. Pr: 0.9855
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAHNHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADYTTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSSAAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINAAVKAYKAQGLGAWGF

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2584
  • protein localization: see SACOL2584
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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