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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS14150 [old locus tag: SACOL2705 ]
  • pan locus tag?: SAUPAN006437000
  • symbol: SACOL_RS14150
  • pan gene symbol?:
  • synonym:
  • product: polysaccharide deacetylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS14150 [old locus tag: SACOL2705 ]
  • symbol: SACOL_RS14150
  • product: polysaccharide deacetylase
  • replicon: chromosome
  • strand: -
  • coordinates: 2777490..2778614
  • length: 1125
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (2777490..2778614) NCBI
  • BioCyc: SACOL_RS14150 BioCyc
  • MicrobesOnline: see SACOL2705

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCATAAAAAATATTTATTGCTATTTTTTCTGTTATTATCAGTTGCATTGTTATATAGC
    TGTTCCAAGCAAAATGAGAAAGCTCAAAATAAATCTGTTAATCCAAATGAGTATGCAAAG
    CATAAATCGAAAATGGATGAACATTGGGAAACATATCACGGCCAAGTTTATCATGTGTTT
    TATCACCCATTAATTACAGACCCAAAAGTTGCGTTTACAGGAGATACACACCAAGCTAAG
    GGCAACAATGATTGGATGATTACTGTAAGTGAATTTAAAAAATCATTGAACGAATTATAT
    AAAAATAATTACATCATCGTTAATCCACATGATGTATACGACTTATCATCTCATCATGTA
    AAAGCCAAAAAAATAAAATTGCCGAAAGGTAAAAAGCCACTAATCATCTCAATTGATGAT
    ATGAATTACTATAGTTACATGAGACACCATGGTTATGCTGACCGTTTAGTACTCGATAAA
    AATAAACATGTTGTTTCTGAAACTACAGACAAAAAAGGCCATGCTACTTATTCTGATGAC
    AATGATATCGTGCCAATTTTAAATAAATTTGTAAAAGAGCATCCAGATTTTTCTTTAAAT
    GGTGAAAAAGGTGTTGTTGCTTTAACCGGTTATGAGGGCGTATTAGGTTATCGCACGAAT
    GAATTAACGAGTAAAGATTATACAAAGAATAAAAAGGCAGCTGAGGAAGTTGTTCGCGCA
    ATGAAACGGGATGGATGGTCATTTGCGAGTCATTCATATGGTCACATTAATTTTGAAAAA
    ACATCTTTAGAAGGTATTAAACGTGATACTAAAAGATGGAAAGATGAAGTTGAACCAATT
    GTAGGTAAAACAGATATGTTTGTATTTCCACATGGCGCACAGGATAGACATACACAAGCG
    TATGATTATTTGGTAGATGAAGCAGAGTTTAAGTTTATTGCAGGTGTCGGTCCAAACAAT
    TTTACAGATATATCAGCAACGAACGTTTACCAAGATAGGGTTGCGATTGATGGTCTAAAC
    TTATTTGAATTTAAATATAAACTCAAACCGTTTTTTAATCCTGAAAATGTATATAGTAAA
    CAAGATAGACGTTACTTTAAAGGGAATCGGGATTATGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS14150 [old locus tag: SACOL2705 ]
  • symbol: SACOL_RS14150
  • description: polysaccharide deacetylase
  • length: 374
  • theoretical pI: 9.01084
  • theoretical MW: 43552.8
  • GRAVY: -0.757754

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination polysaccharide deacetylase family sporulation protein PdaB (TIGR02764; HMM-score: 24.9)
    aromatic cluster surface protein (TIGR04313; HMM-score: 23.3)
    and 4 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan delta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
    Cellular processes Cellular processes Sporulation and germination delta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide deacetylase family protein, PEP-CTERM locus subfamily (TIGR03006; HMM-score: 16.4)
    putative urate catabolism protein (TIGR03212; HMM-score: 12.9)
  • TheSEED: see SACOL2705
  • PFAM:
    GH_CE (CL0158) Polysacc_deac_1; Polysaccharide deacetylase (PF01522; HMM-score: 31.8)
    and 4 more
    no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 15.1)
    DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 15)
    HicA_toxin; HicA toxin of bacterial toxin-antitoxin, (PF07927; HMM-score: 12.7)
    GH_CE (CL0158) DUF2334; Uncharacterized protein conserved in bacteria (DUF2334) (PF10096; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.001313
    • TAT(Tat/SPI): 0.000073
    • LIPO(Sec/SPII): 0.998424
    • Cleavage Site: CS pos: 20-21. LYS-CS. Pr: 0.9986
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSKMDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKNNYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDKNKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYRTNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDEVEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRVAIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE

Experimental data[edit | edit source]

  • experimentally validated: see SACOL2705
  • protein localization: see SACOL2705
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]