PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS14150 [old locus tag: SACOL2705 ]
• pan locus tag^?: SAUPAN006437000
• symbol: SACOL_RS14150
• pan gene symbol^?: —
• synonym:
• product: polysaccharide deacetylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS14150 [old locus tag: SACOL2705 ]
• symbol: SACOL_RS14150
• product: polysaccharide deacetylase
• replicon: chromosome
• strand: -
• coordinates: 2777490..2778614
• length: 1125
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2777490..2778614) NCBI
• BioCyc: SACOL_RS14150 BioCyc
• MicrobesOnline: see SACOL2705

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
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  661
  721
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  841
  901
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  1021
  1081
  
  ATGCATAAAAAATATTTATTGCTATTTTTTCTGTTATTATCAGTTGCATTGTTATATAGC
  TGTTCCAAGCAAAATGAGAAAGCTCAAAATAAATCTGTTAATCCAAATGAGTATGCAAAG
  CATAAATCGAAAATGGATGAACATTGGGAAACATATCACGGCCAAGTTTATCATGTGTTT
  TATCACCCATTAATTACAGACCCAAAAGTTGCGTTTACAGGAGATACACACCAAGCTAAG
  GGCAACAATGATTGGATGATTACTGTAAGTGAATTTAAAAAATCATTGAACGAATTATAT
  AAAAATAATTACATCATCGTTAATCCACATGATGTATACGACTTATCATCTCATCATGTA
  AAAGCCAAAAAAATAAAATTGCCGAAAGGTAAAAAGCCACTAATCATCTCAATTGATGAT
  ATGAATTACTATAGTTACATGAGACACCATGGTTATGCTGACCGTTTAGTACTCGATAAA
  AATAAACATGTTGTTTCTGAAACTACAGACAAAAAAGGCCATGCTACTTATTCTGATGAC
  AATGATATCGTGCCAATTTTAAATAAATTTGTAAAAGAGCATCCAGATTTTTCTTTAAAT
  GGTGAAAAAGGTGTTGTTGCTTTAACCGGTTATGAGGGCGTATTAGGTTATCGCACGAAT
  GAATTAACGAGTAAAGATTATACAAAGAATAAAAAGGCAGCTGAGGAAGTTGTTCGCGCA
  ATGAAACGGGATGGATGGTCATTTGCGAGTCATTCATATGGTCACATTAATTTTGAAAAA
  ACATCTTTAGAAGGTATTAAACGTGATACTAAAAGATGGAAAGATGAAGTTGAACCAATT
  GTAGGTAAAACAGATATGTTTGTATTTCCACATGGCGCACAGGATAGACATACACAAGCG
  TATGATTATTTGGTAGATGAAGCAGAGTTTAAGTTTATTGCAGGTGTCGGTCCAAACAAT
  TTTACAGATATATCAGCAACGAACGTTTACCAAGATAGGGTTGCGATTGATGGTCTAAAC
  TTATTTGAATTTAAATATAAACTCAAACCGTTTTTTAATCCTGAAAATGTATATAGTAAA
  CAAGATAGACGTTACTTTAAAGGGAATCGGGATTATGAAGAATAG
  60
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  1080
  1125
  

⊟Protein

⊟General

• locus tag: SACOL_RS14150 [old locus tag: SACOL2705 ]
• symbol: SACOL_RS14150
• description: polysaccharide deacetylase
• length: 374
• theoretical pI: 9.01084
• theoretical MW: 43552.8
• GRAVY: -0.757754

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination polysaccharide deacetylase family sporulation protein PdaB (TIGR02764; HMM-score: 24.9)
  aromatic cluster surface protein (TIGR04313; HMM-score: 23.3)
  and 4 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan delta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
  Cellular processes Cellular processes Sporulation and germination delta-lactam-biosynthetic de-N-acetylase (TIGR02884; EC 3.5.-.-; HMM-score: 18.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides polysaccharide deacetylase family protein, PEP-CTERM locus subfamily (TIGR03006; HMM-score: 16.4)
  putative urate catabolism protein (TIGR03212; HMM-score: 12.9)
• TheSEED: see SACOL2705
• PFAM:
  GH_CE (CL0158) Polysacc_deac_1; Polysaccharide deacetylase (PF01522; HMM-score: 31.8)
  and 4 more
  no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 15.1)
  DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 15)
  HicA_toxin; HicA toxin of bacterial toxin-antitoxin, (PF07927; HMM-score: 12.7)
  GH_CE (CL0158) DUF2334; Uncharacterized protein conserved in bacteria (DUF2334) (PF10096; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.001313
  • TAT(Tat/SPI): 0.000073
  • LIPO(Sec/SPII): 0.998424
  • Cleavage Site: CS pos: 20-21. LYS-CS. Pr: 0.9986
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446471369 NCBI
• RefSeq: WP_000549223 NCBI
• UniProt: see SACOL2705

⊟Protein sequence

• MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSKMDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKNNYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDKNKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYRTNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDEVEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRVAIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE

⊟Experimental data

• experimentally validated: see SACOL2705
• protein localization: see SACOL2705
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications