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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00340
  • pan locus tag?: SAUPAN001902000
  • symbol: SAOUHSC_00340
  • pan gene symbol?: metC
  • synonym:
  • product: trans-sulfuration enzyme family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00340
  • symbol: SAOUHSC_00340
  • product: trans-sulfuration enzyme family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 353960..355120
  • length: 1161
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACACTTTCAAAAGAGACAGAAGTGATATTCGATTGGCGTAGAGGTGTGGAATATCAT
    TCAGCTAACCCACCACTCTATGATTCTTCAACATTCCATCAAACAAGTCTTGGTGGCGAT
    GTTAAATATGATTATGCACGAAGTGGCAACCCTAACCGTGAACTTTTAGAAGAGAAGTTA
    GCACGATTAGAACAGGGAAAATTCGCTTTTGCTTTTGCATCAGGTATTGCTGCTATTTCA
    GCAGTACTTTTGACTTTCAAATCTGGTGATCATGTCATCTTACCCGATGATGTATATGGC
    GGTACTTTTCGCCTCACTGAGCAAATTTTGAATCGATTTAATATTGAATTTACAACCGTA
    GATACTACAAAGCTCGAACAAATCGAGGGTGCCATTCAATCAAACACAAAATTAATTTAT
    ATCGAAACACCATCGAATCCCTGTTTTAAAATTACTGATATCAAAGCTGTTTCTAAAATA
    GCCGAAAAGCATGAACTACTGGTAGCTGTGGACAATACATTTATGACACCGTTAGGTCAA
    TCACCTTTATTACTTGGCGCTGATATTGTCATTCATAGTGCTACAAAATTTTTAAGTGGA
    CATAGCGATTTAATTGCTGGTGCTGTCATAACTAATAATGAGGCAATTAGTGACGCTCTT
    TATTTAATACAAAATGGTACAGGCAATATGTTATCTGCTCAGGATAGCTGGACACTTGCT
    AAACATTTAAAAACTTTTCCAATCAGATTTAAACAATCTGTCGAAAACGCTCAAAAAATC
    GTGTCATTTTTAATAAAGCAAGATGAAATTTCAGAAGTTTATTATCCGGGACTCACTACT
    GCTCATTTAGAACAAGCTAAAAATGGCGGTGCCGTTATTGGCTTTCGTTTAGCTGATGAG
    TCTAAAGCACAACAATTTGTCGATGCACTGACATTACCACTCGTTTCAGTGAGTCTGGGC
    GGTGTTGAAACGATCCTTTCACATCCAGCAACAATGTCTCACGCTGCACTACCTGAAGAA
    GTGAGACAAGAACGTGGTATCACTTTCGGTCTATTCCGATTAAGTGTTGGTCTCGAAGAT
    CCTGATGAACTCATTGCAGACATCAAATACGCATTAAAGGAGGCATTCAATGAGTCAATT
    CCTCACACAATTGAAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1161

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_00340
  • symbol: SAOUHSC_00340
  • description: trans-sulfuration enzyme family protein
  • length: 386
  • theoretical pI: 5.04033
  • theoretical MW: 42487.9
  • GRAVY: -0.0761658

Function[edit | edit source]

  • TIGRFAM:
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 356)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 345.5)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 335.9)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 294.6)
    and 26 more
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 266.1)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 266.1)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 202.2)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 24.8)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 23.4)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 21.9)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 21.5)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 19.7)
    Unknown function Enzymes of unknown specificity uncharacterized pyridoxal phosphate-dependent enzyme (TIGR01437; HMM-score: 19.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 18.7)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 18.6)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 18.4)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides TDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 17.3)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 15.6)
    Metabolism Energy metabolism Amino acids and amines glycine dehydrogenase (TIGR00461; EC 1.4.4.2; HMM-score: 13.4)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 13.2)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 13.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 12.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 12.9)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 12.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 12.3)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 12.2)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 11.5)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 11.2)
  • TheSEED:  
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Cystathionine beta-lyase (EC 4.4.1.8)
    and 1 more
    Sulfur Metabolism Organic sulfur assimilation L-Cystine Uptake and Metabolism  Cystathionine beta-lyase (EC 4.4.1.8)
  • PFAM:
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 417.5)
    and 7 more
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 34)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 31.3)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 24)
    Met_gamma_lyase; Methionine gamma-lyase (PF06838; HMM-score: 23.5)
    SelA; L-seryl-tRNA selenium transferase (PF03841; HMM-score: 15)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 12.8)
    GG-leader (CL0400) ComC; COMC family (PF03047; HMM-score: 10.3)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:
    SAOUHSC_00520(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SAOUHSC_01757(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SAOUHSC_00187formate acetyltransferase  [1] (data from MRSA252)
    SAOUHSC_00530elongation factor Tu  [1] (data from MRSA252)
    SAOUHSC_00679hypothetical protein  [1] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [1] (data from MRSA252)
    SAOUHSC_01042branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    SAOUHSC_01043dihydrolipoamide dehydrogenase  [1] (data from MRSA252)

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009234
    • TAT(Tat/SPI): 0.001149
    • LIPO(Sec/SPII): 0.002636
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYDYARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGTFRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKIAEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISDALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYPGLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSHAALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: T-box(Met) (transcription antitermination) regulon
    T-box(Met)(5' cis-acting region)important in Amino acid metabolism; transcription unit transferred from N315 data RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]