From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00538
  • pan locus tag?: SAUPAN002328000
  • symbol: SAOUHSC_00538
  • pan gene symbol?: —
  • synonym:
  • product: haloacid dehalogenase-like hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00538
  • symbol: SAOUHSC_00538
  • product: haloacid dehalogenase-like hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 543109..543792
  • length: 684
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAATGGATATTATTTGATAAAGATGGTACGTTAATTGAATTTGATAGAAGTTGGGAA
    AAAATAGGGGTACGATTTGTACAATCATTGCTTGAGACTTTCCCAGTACATAATAAAGAA
    GCTGCTTTAAGACAACTCGGTGTCATTAAAGAATCTATTGATCCAAAATCAGTGATGGGT
    TCAGGATCTTTACAACAAATTATCCAGGCATTTAATGATGTGACGGGACAAGATACAACC
    GACTGGTCCAAGTCAACAAGTCAAAAGCTGGTAGATGAACGTATTCCTGAAATTAATTGG
    GTAGAAGGTGTTAAAGAAGCACTTATCGATTTGAAAGCAAAAGGCTATCAACTTGGTATT
    GTTACGAGTGATACTAAAAAAGGTGTAGAACAATTTTTAGCACATACCAATGCTACCTCG
    TTGTTCGATTTGATCATTTCTACCGAAGCGGATGCCTATGAGAAGCCAAATCCTAAAGTA
    TTATCGCCTTTATTTGAGCAATATAATGTAGATCCTCAGAAAGTAGCTATAGTAGGAGAC
    ACTGCTAATGATATGAAGACAGCAAGTAATGCAAATTTAGGTATGGCAATAGGTGTATTA
    ACAGGTATTGCAACAAAAGAAGAATTACATGAAGCTGATATTATTTTAAATAGTGCGGCA
    GATATTTTAGAAGCTTTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    684

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00538
  • symbol: SAOUHSC_00538
  • description: haloacid dehalogenase-like hydrolase
  • length: 227
  • theoretical pI: 4.35345
  • theoretical MW: 24992.1
  • GRAVY: -0.17489

⊟Function[edit | edit source]

  • TIGRFAM:
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 70.8)
    Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 61.1)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 58.6)
    and 32 more
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 54.9)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 45.6)
    AHBA synthesis associated protein (TIGR01454; HMM-score: 42.9)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 41.7)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 41.2)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 38.5)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 35.5)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 35.5)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides D,D-heptose 1,7-bisphosphate phosphatase (TIGR00213; HMM-score: 33.5)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 31.2)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 30.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 29.2)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphatase (TIGR01261; EC 3.1.3.15; HMM-score: 27.6)
    Metabolism Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 27.4)
    epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 26.9)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 25.7)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 25.6)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 24.8)
    HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 24)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 23)
    Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 21.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 21.9)
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 21)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 20.7)
    DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 19.1)
    phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 17.4)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 15.9)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 14.6)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 14.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 14.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 13.1)
    HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 8.9)
  • TheSEED  :
    • Hydrolase, haloacid dehalogenase-like family
  • PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 99.6)
    and 7 more
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 65)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 40)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 33)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 23)
    Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 17.4)
    PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 16.7)
    Put_Phosphatase; Putative Phosphatase (PF06888; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002681
    • TAT(Tat/SPI): 0.000462
    • LIPO(Sec/SPII): 0.000261
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALRQLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVEGVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKVLSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNSAADILEALN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_00538&oldid=73503"