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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0602 [new locus tag: SACOL_RS03115 ]
  • pan locus tag?: SAUPAN002328000
  • symbol: SACOL0602
  • pan gene symbol?: —
  • synonym:
  • product: HAD superfamily hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0602 [new locus tag: SACOL_RS03115 ]
  • symbol: SACOL0602
  • product: HAD superfamily hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 627938..628621
  • length: 684
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAATGGATATTATTTGATAAAGATGGTACGTTAATTGAATTTGATAGAAGTTGGGAA
    AAAATAGGGGTACGATTTGTACAATCATTGCTTGAGACTTTCCCAGTACATAATAAAGAA
    GCTGCTTTAAGACAACTCGGTGTCATTAAAGAATCTATTGATCCAAAATCAGTGATGGGT
    TCAGGATCTTTACAACAAATTATCCAGGCATTTAATGATGTGACGGGACAAGATACAACC
    GACTGGTCCAAGTCAACAAGTCAAAAGCTGGTAGATGAACGTATTCCTGAAATTAATTGG
    GTAGAAGGTGTTAAAGAAGCACTTATCGATTTGAAAGCAAAAGGCTATCAACTTGGTATT
    GTTACGAGTGATACTAAAAAAGGTGTAGAACAATTTTTAGCACATACCAATGCTACCTCG
    TTGTTCGATTTGATCATTTCTACCGAAGCGGATGCCTATGAGAAGCCAAATCCTAAAGTA
    TTATCGCCTTTATTTGAGCAATATAATGTAGATCCTCAGAAAGTAGCTATAGTAGGAGAC
    ACTGCTAATGATATGAAGACAGCAAGTAATGCAAATTTAGGTATGGCAATAGGTGTATTA
    ACAGGTATTGCAACAAAAGAAGAATTACATGAAGCTGATATTATTTTAAATAGTGCGGCA
    GATATTTTAGAAGCTTTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    684

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0602 [new locus tag: SACOL_RS03115 ]
  • symbol: SACOL0602
  • description: HAD superfamily hydrolase
  • length: 227
  • theoretical pI: 4.35345
  • theoretical MW: 24992.1
  • GRAVY: -0.17489

⊟Function[edit | edit source]

  • TIGRFAM:
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 70.8)
    Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 61.1)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 58.6)
    and 32 more
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 54.9)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 45.6)
    AHBA synthesis associated protein (TIGR01454; HMM-score: 42.9)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 41.7)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 41.2)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 38.5)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 35.5)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 35.5)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides D,D-heptose 1,7-bisphosphate phosphatase (TIGR00213; HMM-score: 33.5)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 31.2)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 30.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 29.2)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphatase (TIGR01261; EC 3.1.3.15; HMM-score: 27.6)
    Metabolism Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 27.4)
    epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 26.9)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 25.7)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 25.6)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 24.8)
    HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 24)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 23)
    Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 21.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 21.9)
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 21)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 20.7)
    DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 19.1)
    phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 17.4)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 15.9)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 14.6)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 14.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 14.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 13.1)
    HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 8.9)
  • TheSEED  :
    • Hydrolase, haloacid dehalogenase-like family
  • PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 99.6)
    and 7 more
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 65)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 40)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 33)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 23)
    Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 17.4)
    PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 16.7)
    Put_Phosphatase; Putative Phosphatase (PF06888; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002681
    • TAT(Tat/SPI): 0.000462
    • LIPO(Sec/SPII): 0.000261
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALRQLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVEGVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKVLSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNSAADILEALN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 1704 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 14.07 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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