PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS03115 [old locus tag: SACOL0602 ]
• pan locus tag^?: SAUPAN002328000
• symbol: SACOL_RS03115
• pan gene symbol^?: —
• synonym:
• product: HAD family hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS03115 [old locus tag: SACOL0602 ]
• symbol: SACOL_RS03115
• product: HAD family hydrolase
• replicon: chromosome
• strand: +
• coordinates: 627938..628621
• length: 684
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (627938..628621) NCBI
• BioCyc: SACOL_RS03115 BioCyc
• MicrobesOnline: see SACOL0602

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAATGGATATTATTTGATAAAGATGGTACGTTAATTGAATTTGATAGAAGTTGGGAA
  AAAATAGGGGTACGATTTGTACAATCATTGCTTGAGACTTTCCCAGTACATAATAAAGAA
  GCTGCTTTAAGACAACTCGGTGTCATTAAAGAATCTATTGATCCAAAATCAGTGATGGGT
  TCAGGATCTTTACAACAAATTATCCAGGCATTTAATGATGTGACGGGACAAGATACAACC
  GACTGGTCCAAGTCAACAAGTCAAAAGCTGGTAGATGAACGTATTCCTGAAATTAATTGG
  GTAGAAGGTGTTAAAGAAGCACTTATCGATTTGAAAGCAAAAGGCTATCAACTTGGTATT
  GTTACGAGTGATACTAAAAAAGGTGTAGAACAATTTTTAGCACATACCAATGCTACCTCG
  TTGTTCGATTTGATCATTTCTACCGAAGCGGATGCCTATGAGAAGCCAAATCCTAAAGTA
  TTATCGCCTTTATTTGAGCAATATAATGTAGATCCTCAGAAAGTAGCTATAGTAGGAGAC
  ACTGCTAATGATATGAAGACAGCAAGTAATGCAAATTTAGGTATGGCAATAGGTGTATTA
  ACAGGTATTGCAACAAAAGAAGAATTACATGAAGCTGATATTATTTTAAATAGTGCGGCA
  GATATTTTAGAAGCTTTAAATTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS03115 [old locus tag: SACOL0602 ]
• symbol: SACOL_RS03115
• description: HAD family hydrolase
• length: 227
• theoretical pI: 4.35345
• theoretical MW: 24992.1
• GRAVY: -0.17489

⊟Function

• TIGRFAM:
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 70.8)
  Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 61.1)
  phosphonatase-like hydrolase (TIGR03351; HMM-score: 58.6)
  and 32 more
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 54.9)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 45.6)
  AHBA synthesis associated protein (TIGR01454; HMM-score: 42.9)
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 41.7)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 41.2)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 38.5)
  haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 35.5)
  HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 35.5)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides D,D-heptose 1,7-bisphosphate phosphatase (TIGR00213; HMM-score: 33.5)
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 31.2)
  Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 30.2)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 29.2)
  Metabolism Amino acid biosynthesis Histidine family histidinol-phosphatase (TIGR01261; EC 3.1.3.15; HMM-score: 27.6)
  Metabolism Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 27.4)
  epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 26.9)
  phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 25.7)
  Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 25.6)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 24.8)
  HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 24)
  pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 23)
  Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 21.9)
  Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 21.9)
  heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 21)
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 20.7)
  DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 19.1)
  phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 17.4)
  phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 15.9)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 14.6)
  HAD phosphatase, family IIIC (TIGR01681; HMM-score: 14.3)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 14.2)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 13.1)
  HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 8.9)
• TheSEED: see SACOL0602
• PFAM:
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 99.6)
  and 7 more
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 65)
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 40)
  HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 33)
  Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 23)
  Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 17.4)
  PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 16.7)
  Put_Phosphatase; Putative Phosphatase (PF06888; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002681
  • TAT(Tat/SPI): 0.000462
  • LIPO(Sec/SPII): 0.000261
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446376929 NCBI
• RefSeq: WP_000454784 NCBI
• UniProt: see SACOL0602

⊟Protein sequence

• MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALRQLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVEGVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKVLSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNSAADILEALN

⊟Experimental data

• experimentally validated: see SACOL0602
• protein localization: see SACOL0602
• quantitative data / protein copy number per cell: see SACOL0602
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0602

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications