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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00654
- pan locus tag?: SAUPAN002527000
- symbol: SAOUHSC_00654
- pan gene symbol?: fhuG
- synonym:
- product: ferrichrome ABC transporter permease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_00654
- symbol: SAOUHSC_00654
- product: ferrichrome ABC transporter permease
- replicon: chromosome
- strand: +
- coordinates: 643035..644051
- length: 1017
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3919945 NCBI
- RefSeq: YP_499213 NCBI
- BioCyc: G1I0R-609 BioCyc
- MicrobesOnline: 1289123 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGATTAGTTCAAATAATAAACGCAGACAATTGATAGCACTGGCTGTTTTTAGCATTCTA
CTATTTCTAGGTTGTACTTGGAGTATTACCTCAGGTGAATACAACATACCTGTTGAAAGA
TTTTTCAAAACTTTAATTGGACAAGGTGATGCCATTGATGAGTTAATCTTATTAGATTTC
AGGTTACCTCGGATGATGATTACTATTTTGGCTGGCGCAGCGCTTAGTATTAGTGGTGCA
ATAGTGCAAAGTGTCACAAAAAATCCAATAGCTGAACCAGGTATATTAGGTATTAACGCA
GGTGGCGGATTTGCAATCGCATTATTTATTGCAATTGGTAAAATTAATGCTGACAACTTT
GTTTATGTACTGCCGTTAATAAGTATACTAGGTGGTATCACCACTGCATTGATTATTTTT
ATTTTCAGTTTTAATAAAAATGAAGGTGTTACACCTGCGAGTATGGTATTAATAGGTGTA
GGTTTACAAACAGCATTATATGGTGGCTCAATTACAATTATGTCAAAATTTGATGATAAG
CAATCTGATTTCATCGCTGCTTGGTTTGCAGGTAATATTTGGGGTGACGAATGGCCATTT
GTCATTGCATTTTTACCGTGGGTGTTGATTATTATTCCTTACTTACTATTTAAATCGAAT
ACACTAAATATTATTCATACGGGTGATAATATTGCACGAGGTCTAGGTGTAAGGTTAAGC
AGAGAACGTTTAATATTATTCTTTATCGCAGTGATGTTATCATCTGCTGCTGTAGCAGTA
GCAGGTTCAATTTCGTTTATCGGATTAATGGGTCCGCATATTGCCAAACGTATCGTTGGA
CCACGTCACCAGTTGTTTTTACCAATTGCCATTTTAGTAGGGGCATGTTTACTTGTTATA
GCTGATACAATTGGCAAAATTGTATTACAACCAGGTGGGGTTCCAGCAGGTATTGTCGTA
GCAATTATTGGTGCACCGTATTTCTTATATTTAATGTACAAAACGAAAAATGTATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00654
- symbol: SAOUHSC_00654
- description: ferrichrome ABC transporter permease
- length: 338
- theoretical pI: 9.75188
- theoretical MW: 36278.1
- GRAVY: 0.897633
⊟Function[edit | edit source]
- TIGRFAM: proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 163.8)and 1 moreEnergy metabolism Electron transport cytochrome c oxidase, subunit IVB (TIGR02908; EC 1.9.3.1; HMM-score: 4.6)
- TheSEED :
- Ferrichrome ABC transporter, permease protein FhuG
- PFAM: Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 291.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 9
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide LIPO(Sec/SPII) length 24 aa
- SP(Sec/SPI): 0.01306
- TAT(Tat/SPI): 0.003282
- LIPO(Sec/SPII): 0.973835
- Cleavage Site: CS pos: 24-25. FLG-CT. Pr: 0.9727
- predicted transmembrane helices (TMHMM): 9
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKTLIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGGGFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGVGLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFKSNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAKRIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYKTKNV
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: Fur* (repression) regulon
Fur* (TF) important in Iron homeostasis; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)