From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0635 [new locus tag: SAUSA300_RS03405 ]
  • pan locus tag?: SAUPAN002527000
  • symbol: fhuG
  • pan gene symbol?: fhuG
  • synonym:
  • product: ferrichrome transport permease fhuG

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0635 [new locus tag: SAUSA300_RS03405 ]
  • symbol: fhuG
  • product: ferrichrome transport permease fhuG
  • replicon: chromosome
  • strand: +
  • coordinates: 709965..710981
  • length: 1017
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTAGTTCAAATAATAAACGCAGACAATTGATAGCACTGGCTGTTTTTAGCATTCTA
    CTATTTCTAGGTTGTACTTGGAGTATTACCTCAGGTGAATACAACATACCTGTTGAAAGA
    TTTTTCAAAACTTTAATTGGACAAGGTGATGCCATTGATAAGTTAATCTTATTAGATTTC
    AGGTTACCTCGGATGATGATTACTATTTTGGCTGGCGCAGCGCTTAGTATTAGTGGTGCA
    ATAGTGCAAAGTGTCACAAAAAATCCAATAGCTGAACCAGGTATATTAGGTATTAACGCA
    GGTGGCGGATTTGCAATCGCATTATTTATTGCAATTGGTAAAATTAATGCTGACAACTTT
    GTTTATGTACTGCCGTTAATAAGTATACTAGGTGGTATCACCACTGCATTGATTATTTTT
    ATTTTCAGTTTTAATAAAAATGAAGGTGTTACACCTGCGAGTATGGTATTAATAGGTGTA
    GGTTTACAAACAGCATTATATGGTGGCTCAATTACAATTATGTCAAAATTTGATGATAAG
    CAATCTGATTTCATCGCTGCTTGGTTTGCAGGTAATATTTGGGGTGACGAATGGCCATTT
    GTCATTGCATTTTTACCGTGGGTGTTGATTATTATTCCTTACTTACTATTTAAATCGAAT
    ACACTAAATATTATTCATACGGGTGATAATATTGCACGAGGTCTAGGTGTAAGGTTAAGC
    AGAGAACGTTTAATATTATTCTTTATCGCAGTGATGTTATCATCTGCTGCTGTAGCAGTA
    GCAGGTTCAATTTCGTTTATCGGATTAATGGGTCCGCATATTGCCAAACGTATCGTTGGA
    CCACGTCACCAGTTGTTTTTACCAATTGCCATTTTAGTAGGGGCATGTTTACTTGTTATA
    GCTGATACAATTGGCAAAATTGTATTACAACCAGGTGGGGTTCCAGCAGGTATTGTCGTA
    GCAATTATTGGTGCACCGTATTTCTTATATTTAATGTACAAAACGAAAAATGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1017

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0635 [new locus tag: SAUSA300_RS03405 ]
  • symbol: FhuG
  • description: ferrichrome transport permease fhuG
  • length: 338
  • theoretical pI: 10.0239
  • theoretical MW: 36277.1
  • GRAVY: 0.89645

⊟Function[edit | edit source]

  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 162.6)
    and 1 more
    Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit IVB (TIGR02908; EC 1.9.3.1; HMM-score: 4.6)
  • TheSEED  :
    • ABC-type Fe3+-siderophore transport system, permease 2 component
    Stress Response Stress Response - no subcategory Flavohaemoglobin  ABC-type Fe3+-siderophore transport system, permease 2 component
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 291.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide LIPO(Sec/SPII) length 24 aa
    • SP(Sec/SPI): 0.013241
    • TAT(Tat/SPI): 0.003488
    • LIPO(Sec/SPII): 0.973045
    • Cleavage Site: CS pos: 24-25. FLG-CT. Pr: 0.9720
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKTLIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGGGFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGVGLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFKSNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAKRIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYKTKNV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAUSA300_0635&oldid=89544"