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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00984
  • pan locus tag?: SAUPAN003247000
  • symbol: SAOUHSC_00984
  • pan gene symbol?: menH
  • synonym:
  • product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00984
  • symbol: SAOUHSC_00984
  • product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 955930..956733
  • length: 804
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACACATTATAAATTTTATGAAGCAAACGTTGAGACCAATCAAGTTTTAGTATTTCTG
    CATGGATTTCTTAGCGACAGCCGTACTTATCATAATCACATCGAAAAATTTACTGATAAC
    TATCATGTCATCACTATAGACTTACCAGGCCATGGCGAAGATCAGTCTTCAATGGATGAA
    ACGTGGAATTTTGATTATATTACGACGTTGTTAGACCGAATTTTAGATAAATATAAAGAT
    AAATCAATAACATTGTTTGGATATTCAATGGGTGGGCGTGTTGCATTATATTATGCAATT
    AATGGTCACATCCCTATATCTAATTTGATATTAGAAAGTACGTCACCAGGTATTAAAGAA
    GAAGCAAATCAATTGGAACGCCGTCTTGTTGATGATGCACGTGCTAAAGTATTAGACATA
    GCAGGTATTGAATTATTTGTTAATGATTGGGAAAAGTTGCCATTATTTCAATCGCAACTA
    GAATTACCAGTTGAAATACAACATCAAATAAGACAACAACGATTGTCTCAATCGCCACAT
    AAAATGGCCAAAGCATTAAGAGATTATGGTACAGGTCAAATGCCAAACTTATGGCCGCGC
    CTGAAAGAAATTAAAGTACCAACATTAATATTAGCTGGAGAATATGATGAAAAATTTGTA
    CAGATTGCGAAAAAAATGGCAAATTTAATTCCTAATAGTAAATGTAAATTAATTTCTGCT
    ACAGGTCATACAATTCATGTGGAAGATAGTGATGAATTTGATACAATGATATTAGGATTT
    TTAAAGGAGGAGCAAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

Protein[edit | edit source]

Protein Data Bank: 2XMZ

General[edit | edit source]

  • locus tag: SAOUHSC_00984
  • symbol: SAOUHSC_00984
  • description: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
  • length: 267
  • theoretical pI: 5.55003
  • theoretical MW: 30784.8
  • GRAVY: -0.409738

Function[edit | edit source]

  • reaction:
    EC 4.2.99.20?  ExPASy
    2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 300.5)
    and 10 more
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 83.3)
    proline-specific peptidase (TIGR01250; HMM-score: 78.7)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 61.6)
    pyrimidine utilization protein D (TIGR03611; HMM-score: 60.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 55.9)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 39.8)
    Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 39.1)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 22.1)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 19.5)
    Plasmodium subtelomeric family (TIGR01607; HMM-score: 12.3)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone and Phylloquinone Biosynthesis  2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (EC 4.2.99.20)
    and 1 more
    Menaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate  2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (EC 4.2.99.20)
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 96.7)
    and 17 more
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 64.8)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 61.9)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 25.5)
    Ndr; Ndr family (PF03096; HMM-score: 23.7)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 19.9)
    DUF2974; Protein of unknown function (DUF2974) (PF11187; HMM-score: 19.2)
    PGAP1; PGAP1-like protein (PF07819; HMM-score: 19)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 18.3)
    DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 17.6)
    Esterase; Putative esterase (PF00756; HMM-score: 16.7)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 15.7)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 15.4)
    AB_hydrolase (CL0028) UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 14.4)
    DUF900; Alpha/beta hydrolase of unknown function (DUF900) (PF05990; HMM-score: 13.2)
    no clan defined DUF2183; Uncharacterized conserved protein (DUF2183) (PF09949; HMM-score: 12.9)
    AB_hydrolase (CL0028) BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 12.8)
    Chlorophyllase2; Chlorophyllase enzyme (PF12740; HMM-score: 10.9)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006007
    • TAT(Tat/SPI): 0.000299
    • LIPO(Sec/SPII): 0.001732
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVITIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAINGHIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQLELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEKFVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]