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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01419
  • pan locus tag?: SAUPAN003833000
  • symbol: SAOUHSC_01419
  • pan gene symbol?: arlS
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01419
  • symbol: SAOUHSC_01419
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1360281..1361636
  • length: 1356
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACAAAACGTAAATTGCGCAATAACTGGATTATTGTTACCACGATGATTACGTTTGTC
    ACGATATTTTTGTTTTGTTTAATTATTATTTTTTTCTTGAAAGATACACTGCATAATAGT
    GAGCTTGATGATGCAGAACGAAGCTCAAGCGATATTAATAATTTATTTCATTCTAAGCCT
    GTTAAAGATATATCTGCATTAGACTTGAATGCATCTTTAGGTAATTTTCAAGAGATAATT
    ATTTATGATGAGCATAATAATAAATTATTTGAGACATCGAATGATAACACAGTGAGAGTT
    GAACCAGGTTATGAACACCGTTATTTTGACCGCGTAATAAAAAAACGCTATAAAGGCATT
    GAATATTTAATTATTAAAGAACCAATTACAACGCAAGATTTCAAAGGGTATAGCTTGTTA
    ATTCATTCACTAGAAAATTATGATAACATCGTAAAATCATTGTATATCATTGCGCTGGCA
    TTTGGAGTGATTGCAACAATTATAACTGCCACAATCAGTTATGTATTTTCAACACAAATT
    ACTAAACCGCTTGTCAGTTTATCAAATAAAATGATTGAGATTCGACGAGATGGTTTTCAA
    AATAAATTGCAATTAAATACAAATTATGAAGAAATAGATAATTTAGCAAATACGTTTAAT
    GAGATGATGAGCCAAATTGAAGAATCATTTAATCAACAAAGACAATTTGTTGAAGATGCG
    TCACATGAATTACGAACACCATTACAAATTATTCAAGGTCATTTAAATTTGATTCAGCGA
    TGGGGAAAAAAAGACCCAGCAGTATTAGAAGAATCGTTAAATATTTCTATTGAAGAAATG
    AATCGTATCATAAAATTAGTCGAAGAATTACTTGAATTGACTAAAGGAGATGTAAATGAC
    ATTTCTTCTGAAGCACAGACCGTGCATATTAATGATGAAATTCGCTCGCGAATACACTCA
    TTAAAACAATTGCATCCTGATTATCAATTTGATACGGATCTGACATCTAAAAATCTAGAA
    ATTAAAATGAAACCTCATCAATTCGAACAATTATTTTTAATCTTTATTGATAATGCAATC
    AAATATGATGTGAAGAATAAGAAAATTAAAGTTAAGACAAGGTTAAAAAATAAGCAAAAA
    ATAATTGAAATTACAGATCATGGAATTGGTATTCCAGAGGAAGATCAAGATTTCATTTTT
    GATCGCTTTTATCGAGTGGATAAATCTCGTTCAAGAAGTCAAGGCGGTAATGGACTCGGA
    TTATCTATTGCTCAAAAAATCATTCAATTAAACGGAGGATCGATTAAAATTAAAAGTGAA
    ATTAACAAAGGAACAACGTTTAAAATCATATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01419
  • symbol: SAOUHSC_01419
  • description: hypothetical protein
  • length: 451
  • theoretical pI: 6.97701
  • theoretical MW: 52399.7
  • GRAVY: -0.33592

Function[edit | edit source]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 197.2)
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 177.3)
    and 8 more
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 91)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 89)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.5)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 51.5)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 49.9)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 49.9)
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.1)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.1)
  • TheSEED:  
    Two component system histidine kinase ArlS
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 88.5)
    and 11 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 51.9)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 25.6)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 22.1)
    no clan defined HAMP; HAMP domain (PF00672; HMM-score: 13.7)
    KaiA_RbsU (CL0637) RsbU_N; Phosphoserine phosphatase RsbU, N-terminal domain (PF08673; HMM-score: 13.4)
    no clan defined Clusterin; Clusterin (PF01093; HMM-score: 13)
    DUF4199; Protein of unknown function (DUF4199) (PF13858; HMM-score: 11.9)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 11.4)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 11.2)
    no clan defined MukF_C; MukF C-terminal domain (PF17193; HMM-score: 11.1)
    DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 8.5)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009038
    • TAT(Tat/SPI): 0.000301
    • LIPO(Sec/SPII): 0.004974
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDDAERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEPGYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALAFGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANTFNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNISIEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDLTSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIPEEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKIIF

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]

B Fournier, R Aras, D C Hooper
Expression of the multidrug resistance transporter NorA from Staphylococcus aureus is modified by a two-component regulatory system.
J Bacteriol: 2000, 182(3);664-71
[PubMed:10633099] [WorldCat.org] [DOI] (P p)
B Fournier, D C Hooper
A new two-component regulatory system involved in adhesion, autolysis, and extracellular proteolytic activity of Staphylococcus aureus.
J Bacteriol: 2000, 182(14);3955-64
[PubMed:10869073] [WorldCat.org] [DOI] (P p)
B Fournier, A Klier, G Rapoport
The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureus.
Mol Microbiol: 2001, 41(1);247-61
[PubMed:11454217] [WorldCat.org] [DOI] (P p)
S S Ingavale, W Van Wamel, A L Cheung
Characterization of RAT, an autolysis regulator in Staphylococcus aureus.
Mol Microbiol: 2003, 48(6);1451-66
[PubMed:12791130] [WorldCat.org] [DOI] (P p)
Adhar C Manna, Susham S Ingavale, MaryBeth Maloney, Willem van Wamel, Ambrose L Cheung
Identification of sarV (SA2062), a new transcriptional regulator, is repressed by SarA and MgrA (SA0641) and involved in the regulation of autolysis in Staphylococcus aureus.
J Bacteriol: 2004, 186(16);5267-80
[PubMed:15292128] [WorldCat.org] [DOI] (P p)
Bénédicte Fournier, André Klier
Protein A gene expression is regulated by DNA supercoiling which is modified by the ArlS-ArlR two-component system of Staphylococcus aureus.
Microbiology (Reading): 2004, 150(Pt 11);3807-3819
[PubMed:15528666] [WorldCat.org] [DOI] (P p)
Alejandro Toledo-Arana, Nekane Merino, Marta Vergara-Irigaray, Michel Débarbouillé, José R Penadés, Iñigo Lasa
Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system.
J Bacteriol: 2005, 187(15);5318-29
[PubMed:16030226] [WorldCat.org] [DOI] (P p)