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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01427
  • pan locus tag?: SAUPAN003849000
  • symbol: SAOUHSC_01427
  • pan gene symbol?: ctpA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01427
  • symbol: SAOUHSC_01427
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1365701..1367191
  • length: 1491
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGATAAGCAACACACATCTTCATCCGATGATGAACGCGCTGAAATTGCAACAAGC
    AATCAAGACCAAGAAACTAATTCATCGAAACGCGTTCACTTAAAACGTTGGCAATTCATA
    TCAATATTAATTGGTACAATCCTAATAACAGCTGTCATCACAGTTGTTGCATATATTTTT
    ATAAATCAAAAAATAAGTGGTTTAAACAAAACTGATCAAGCAAACTTAAATAAAATTGAA
    AATGTGTATAAAATCTTAAATAGTGATTATTACAAAAAACAGGACTCTGACAAGTTAAGT
    AAAGCTGCAATTGATGGCATGGTCAAAGAATTAAAAGATCCTTATTCTGAATATTTAACA
    AAAGAACAAACGAAATCCTTTAATGAAGGTGTTTCAGGTGATTTTGTAGGTATTGGTGCA
    GAAATGCAAAAGAAAAATGATCAAATTATGGTTACTAGTCCTATGAAGGGATCTCCAGCA
    GAACGTGCTGGCATTCGTCCTAAAGATGTCATTACTAAAGTAAATGGAAAATCAATTAAA
    GGTAAAGCATTAGATGAAGTTGTCAAAGATGTTCGTGGTAAAGAAAACACTGAAGTCACT
    TTAACTGTTCAACGAGGTAGTGAAGAAAAAGACGTTAAGATTAAACGTGAAAAAATTCAT
    GTTAAAAGTGTTGAGTATAAGAAAAAAGGTAAAGTTGGAGTTATTACTATTAATAAATTC
    CAGAATGATACATCAGGTGAATTGAAAGATGCAGTTCTAAAAGCTCACAAAGATGGTTTG
    AAAAAGATTGTTTTAGATTTAAGAAATAATCCAGGTGGACTACTAGATGAAGCTGTTAAA
    ATGGCAAATATTTTTATCGATAAAGGAAAAACTGTTGTTAAACTAGAAAAAGGTAAAGAT
    ACTGAAGCAATTCAAACTTCTAATGATGCGTTAAAAGAAGCGAAAGACATGGATATATCC
    ATCTTAGTGAATGAAGGTTCAGCTAGCGCTTCTGAAGTGTTTACTGGTGCGCTAAAAGAC
    TATAATAAAGCTAAAGTTTATGGGTCAAAAACATTCGGCAAAGGTGTCGTACAAACTACA
    AGAGAGTTTAAGGATGGTTCATTGTTAAAATATACTGAAATGAAATGGTTAACGCCAGAT
    GGTCATTATATTCACGGTAAAGGCATTAAACCAGACGTTACTATTGACACACCTAAATAT
    CAATCTTTAAATGTCATTCCTAATACGAAAACATTTAAAGTTGGAGACGATGATAAAAAT
    ATTAAAACTATTAAAATTGGTTTATCAGCTTTAGGTTATAAAGTTGATAATGAATCAACG
    CAATTTGATAAAGCTTTAGAAAATCAAGTTAAAGCTTTCCAACAAGCGAATAAACTTGAG
    GTAACTGGTGAATTTAATAAAGAAACGAATAATAAATTTACTGAGTTATTAGTTGAAAAA
    GCTAATAAACATGATGATGTTCTCGATAAGTTGATTAATATTTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01427
  • symbol: SAOUHSC_01427
  • description: hypothetical protein
  • length: 496
  • theoretical pI: 9.7491
  • theoretical MW: 55260.6
  • GRAVY: -0.634476

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 344)
    Genetic information processing Protein fate Protein modification and repair C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 344)
    and 8 more
    putative carboxyl-terminal-processing protease, deltaproteobacterial (TIGR03900; EC 3.4.21.-; HMM-score: 264.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 36.4)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 36.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 28.8)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 20.2)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 20.2)
    Cellular processes Cellular processes Sporulation and germination spore cortex-lytic enzyme (TIGR02869; HMM-score: 18.7)
    Signal transduction Regulatory functions Protein interactions anti-anti-sigma factor (TIGR00377; HMM-score: 12.1)
  • TheSEED  :
    • Carboxyl-terminal protease (EC 3.4.21.102)
  • PFAM:
    ClpP_crotonase (CL0127) Peptidase_S41; Peptidase family S41 (PF03572; HMM-score: 165.5)
    and 7 more
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 56.2)
    PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 51.7)
    PGBD (CL0244) PG_binding_1; Putative peptidoglycan binding domain (PF01471; HMM-score: 46.5)
    PDZ-like (CL0466) Tricorn_PDZ; Tricorn protease PDZ domain (PF14685; HMM-score: 17)
    PGBD (CL0244) PG_binding_2; Putative peptidoglycan binding domain (PF08823; HMM-score: 14.6)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 12.7)
    no clan defined DUF3394; Domain of unknown function (DUF3394) (PF11874; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002498
    • TAT(Tat/SPI): 0.000562
    • LIPO(Sec/SPII): 0.00446
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILIGTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKAAIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPAERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREKIHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLDEAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVFTGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPDVTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQVKAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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