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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01751
  • pan locus tag?: SAUPAN004248000
  • symbol: ruvA
  • pan gene symbol?: ruvA
  • synonym:
  • product: Holliday junction DNA helicase RuvA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01751
  • symbol: ruvA
  • product: Holliday junction DNA helicase RuvA
  • replicon: chromosome
  • strand: -
  • coordinates: 1655840..1656442
  • length: 603
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTACGCGTATGTCAAAGGTAAGTTAACACATTTATATCCTACACACGTAGTTGTTGAA
    ACTGCTGGTGTTGGTTATGAAATTCAAACACCAAATTCTTATCGTTTTCAAAAGCATCTA
    GATCATGAAGTTTTAATTCATACATCTTTAATTGTTCGTGAAGATGCACAATTATTGTAT
    GGATTTAGTAGTGAAGAAGAGAAAGATATGTTCTTGAGTTTAATTAAAGTTACTGGTATT
    GGTCCGAAATCAGCTTTAGCTATTTTAGCGACAAGTACGCCTAATGAAGTAAAACGTGCC
    ATTGAAAATGAAAATGATACGTATTTAACTAAATTCCCAGGAATTGGTAAGAAAACGGCA
    AGACAGATTGTCTTAGATTTAAAAGGTAAAGTGAAAATTACTGAAGAAGATAGCGATTCA
    TTATTACAAGTAGACGCTACTTCGACGGTGCAAGATCAATTCGTGCAAGAAGCAATGTTA
    GCGTTAGAAGCATTAGGTTATTCTAAACGAGAGCTTGCAAAAGTTGAGAAAACGTTAAAT
    AAAAATAAATATGACTCAGTTGATGAAGCTGTTAAGGCAGGTCTTCAATTAGTTGTATCT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    603

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01751
  • symbol: RuvA
  • description: Holliday junction DNA helicase RuvA
  • length: 200
  • theoretical pI: 6.03453
  • theoretical MW: 22262.3
  • GRAVY: -0.246

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 174)
    and 3 more
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS13 (TIGR03631; HMM-score: 16.1)
    Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 13.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair recombination protein RecR (TIGR00615; HMM-score: 12.3)
  • TheSEED  :
    • Holliday junction DNA helicase RuvA
    DNA Metabolism DNA recombination RuvABC plus a hypothetical  Holliday junction DNA helicase RuvA
    and 1 more
    DNA Metabolism DNA replication DNA-replication  Holliday junction DNA helicase RuvA
  • PFAM:
    OB (CL0021) RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 73.2)
    and 11 more
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 55)
    UBA (CL0214) RuvA_C; RuvA, C-terminal domain (PF07499; HMM-score: 39.1)
    HHH (CL0198) HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 27.5)
    IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 23.6)
    H2TH (CL0303) Ribosomal_S13; Ribosomal protein S13/S18 (PF00416; HMM-score: 20.6)
    HHH (CL0198) HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 17.8)
    HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 16.2)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 13.3)
    HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 13.3)
    HHH_4; Helix-hairpin-helix containing domain (PF14490; HMM-score: 12.9)
    DNA_pol_lambd_f; Fingers domain of DNA polymerase lambda (PF10391; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003512
    • TAT(Tat/SPI): 0.000142
    • LIPO(Sec/SPII): 0.001493
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEVLIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIENENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAMLALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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