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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01877
- pan locus tag?: SAUPAN004446000
- symbol: SAOUHSC_01877
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01877
- symbol: SAOUHSC_01877
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 1792814..1793767
- length: 954
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3921766 NCBI
- RefSeq: YP_500380 NCBI
- BioCyc: G1I0R-1746 BioCyc
- MicrobesOnline: 1290294 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGGGCAATCATTTCCAATACGCTTTTGAAAACAAACGTTATCACACATGGAATTACCAT
TTAAAAAATAAATTTGGACAAAAAATATTTAAAGTTGCATTGGATGGCGGGTTTGACTGT
CCTAACCGCGATGGCACTGTAGCACATGGTGGATGTACATTTTGTTCTGCTGCAGGTAGC
GGAGACTTTGCAGGTAATCGTGCAGATTCAATCGCAGTACAATTTAAAGAAATTAAAGAA
AAGATGCATGAGAAATGGCACGAAGGAAAATATATTGCTTATTTTCAGGCATTTACAAAT
ACACATGCACCGGTTGAAGTATTAAAAGAAAAATTCGAACCTGTACTTAAAGAACCGGGT
GTTGTGGGATTATCTATTGGTACGCGTCCTGACTGTCTACCAGACGATGTTGTCGAATAT
TTAGCAGATTTGAATCAACGAACATACTTATGGGTTGAATTAGGACTACAAACAATCCAT
CAGTCAACATCTGATTTAATCAATCGTGCCCATGATATGAAAACTTATTATGATGGTGTG
GCAAAATTACGTAAGCATAATATCAATGTATGTACACACATCATTAATGGCTTACCTGGC
GAAGACTATGACATGATGATGGCTACTGCCAAAGAAGTTGCACAAATGGATGTACAAGGT
ATTAAAATTCATTTACTTCATTTGTTAAAAGGTACACCGATGGTAAAACAATACGATAAA
GGTTTATTAACTTTTATGACTCAAGAAGAGTACACAAACCTAGTTGTGGACCAATTAGAA
GTGATTCCCCCTGAAATGATCGTTCACCGAATTACCGGTGATGGTCCAATAGATATCATG
GTAGGTCCAATGTGGAGTGTTAATAAATGGGAAGTATTAAATGGCATCGATGCTGAATTA
GCACGTAGAAATTCTTATCAAGGCTTGCGTTACAAGTCTAAGGTGAAGCAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01877
- symbol: SAOUHSC_01877
- description: hypothetical protein
- length: 317
- theoretical pI: 7.03345
- theoretical MW: 36053.1
- GRAVY: -0.393691
⊟Function[edit | edit source]
- TIGRFAM: Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 433.5)and 15 moreTranscription DNA-dependent RNA polymerase radical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 63)Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 39.6)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin coproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 38.8)putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 32)radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 28.2)Biosynthesis of cofactors, prosthetic groups, and carriers Biotin biotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 27.3)Hypothetical proteins Conserved radical SAM enzyme, TIGR01210 family (TIGR01210; HMM-score: 24.4)Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 22.9)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin oxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 22.8)Protein synthesis tRNA and rRNA base modification tRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 19.6)Cellular processes Toxin production and resistance radical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 18.3)Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme, archaeal-type (TIGR01578; HMM-score: 15.1)hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 14.7)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 12.3)
- TheSEED :
- [4Fe-4S]-AdoMet protein YtqA
- PFAM: no clan defined Radical_SAM_C; Radical_SAM C-terminal domain (PF16199; HMM-score: 82.9)and 1 moreTIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 52.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.251462
- TAT(Tat/SPI): 0.001225
- LIPO(Sec/SPII): 0.00343
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3] Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)