⊟Summary[edit | edit source]
- pan ID?: SAUPAN004446000
- symbol?: —
- synonym:
- description?: TIGR01212 family radical SAM protein
- TIGR01212 family radical SAM protein
- Fe-S oxidoreductase, putative
- Fe-S oxidoreductase
- putative Fe-S oxidoreductase
- radical SAM protein
- radical SAM protein, family
- radical SAM superfamily protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 4693950..4694903
- synteny block?: BlockID0034430
- occurrence?: in 100% of 34 strains
mnmL : tRNA 5-carboxymethylaminomethyl-2-thiouridine(34) oxidative decarboxylase MnmL [1]
Transfer RNAs containing uridine at wobble anticodon position 34 often require modifications to provide maximal fidelity during protein synthesis starting from a shared 5-carboxymethylaminomethyl-2-thiouridine(34) (“cmnm5s2U”, “$”) generated by MnmAEG. One such modification involves SAM-dependent oxidative decarboxylation of 5-carboxymethylaminomethyl-2-thiouridine(34) to generate 5-aminomethyl-2-thiouridine(34) and subsequent SAM-dependent methylation to 5-methylaminomethyl-2-thiouridine(34) by the MnmLM complex. A small amount of 5-aminomethyl-2-thiouridine(34) can also be provided by MnmEG using ammonium as a substrate. These two enzymatic activities are shared in the bifunctional enzyme MnmC in gram-negative bacteria.
tRNA : cmnm5s2U (34) → nm5s2U (34)
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
COL | |
N315 | |
NCTC8325 | |
Newman | |
USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
N315 MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
NCTC8325 MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
Newman MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
USA300_FPR3757 MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
************************************************************
COL GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
N315 GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
NCTC8325 GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
Newman GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
USA300_FPR3757 GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
************************************************************
COL VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
N315 VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
NCTC8325 VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
Newman VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
USA300_FPR3757 VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
************************************************************
COL AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
N315 AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
NCTC8325 AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
Newman AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
USA300_FPR3757 AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
************************************************************
COL GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
N315 GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
NCTC8325 GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
Newman GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
USA300_FPR3757 GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
************************************************************
COL ARRNSYQGLRYKSKVKQ
N315 ARRNSYQGLRYKSKVKQ
NCTC8325 ARRNSYQGLRYKSKVKQ
Newman ARRNSYQGLRYKSKVKQ
USA300_FPR3757 ARRNSYQGLRYKSKVKQ
*****************
- ↑ Marshall Jaroch, Guangxin Sun, Ho-Ching Tiffany Tsui, Colbie Reed, Jingjing Sun, Marko Jörg, Malcolm E Winkler, Kelly C Rice, Agnieszka Dziergowska, Troy A Stich, Peter C Dedon, Patricia C Dos Santos, Valérie de Crécy-Lagard
Alternate routes to mnm 5s 2U synthesis in Gram-positive bacteria.
J Bacteriol: 2024, 206(4);e0045223
[PubMed:38551342] [WorldCat.org] [DOI] (I p)