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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004446000
- symbol?: —
- synonym:
- description?: TIGR01212 family radical SAM protein
- TIGR01212 family radical SAM protein
- Fe-S oxidoreductase, putative
- Fe-S oxidoreductase
- putative Fe-S oxidoreductase
- radical SAM protein
- radical SAM protein, family
- radical SAM superfamily protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 4693950..4694903
- synteny block?: BlockID0034430
- occurrence?: in 100% of 34 strains
mnmL : tRNA 5-carboxymethylaminomethyl-2-thiouridine(34) oxidative decarboxylase MnmL [1]
Transfer RNAs containing uridine at wobble anticodon position 34 often require modifications to provide maximal fidelity during protein synthesis starting from a shared 5-carboxymethylaminomethyl-2-thiouridine(34) (“cmnm5s2U”, “$”) generated by MnmAEG. One such modification involves SAM-dependent oxidative decarboxylation of 5-carboxymethylaminomethyl-2-thiouridine(34) to generate 5-aminomethyl-2-thiouridine(34) and subsequent SAM-dependent methylation to 5-methylaminomethyl-2-thiouridine(34) by the MnmLM complex. A small amount of 5-aminomethyl-2-thiouridine(34) can also be provided by MnmEG using ammonium as a substrate. These two enzymatic activities are shared in the bifunctional enzyme MnmC in gram-negative bacteria.
tRNA : cmnm5s2U (34) → nm5s2U (34)
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1718
08BA02176:
C248_1807
11819-97:
MS7_1766
6850:
RSAU_001617
71193:
ST398NM01_1813
ECT-R 2:
ECTR2_1600
ED133:
SAOV_1746
ED98:
SAAV_1771
HO 5096 0412:
SAEMRSA15_16680
JH1:
SaurJH1_1850
JH9:
SaurJH9_1815
JKD6008:
SAA6008_01732
JKD6159:
SAA6159_01683
JSNZ:
JSNZ_001750
LGA251:
SARLGA251_16500
M013:
M013TW_1776
MRSA252:
SAR1845
MSHR1132:
SAMSHR1132_16100
MSSA476:
SAS1686
Mu3:
SAHV_1748
Mu50:
SAV1762
MW2:
MW1703
RF122:
SAB1620
ST398:
SAPIG1813
T0131:
SAT0131_01871
TCH60:
HMPREF0772_11391
TW20:
SATW20_17500
USA300_TCH1516:
USA300HOU_1751
VC40:
SAVC_08010
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
N315 MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
NCTC8325 MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
Newman MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
USA300_FPR3757 MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGS
************************************************************
COL GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
N315 GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
NCTC8325 GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
Newman GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
USA300_FPR3757 GDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPG
************************************************************
COL VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
N315 VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
NCTC8325 VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
Newman VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
USA300_FPR3757 VVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGV
************************************************************
COL AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
N315 AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
NCTC8325 AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
Newman AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
USA300_FPR3757 AKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDK
************************************************************
COL GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
N315 GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
NCTC8325 GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
Newman GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
USA300_FPR3757 GLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAEL
************************************************************
COL ARRNSYQGLRYKSKVKQ
N315 ARRNSYQGLRYKSKVKQ
NCTC8325 ARRNSYQGLRYKSKVKQ
Newman ARRNSYQGLRYKSKVKQ
USA300_FPR3757 ARRNSYQGLRYKSKVKQ
*****************
- ↑ Marshall Jaroch, Guangxin Sun, Ho-Ching Tiffany Tsui, Colbie Reed, Jingjing Sun, Marko Jörg, Malcolm E Winkler, Kelly C Rice, Agnieszka Dziergowska, Troy A Stich, Peter C Dedon, Patricia C Dos Santos, Valérie de Crécy-Lagard
Alternate routes to mnm 5s 2U synthesis in Gram-positive bacteria.
J Bacteriol: 2024, 206(4);e0045223
[PubMed:38551342] [WorldCat.org] [DOI] (I p)