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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1810 [new locus tag: SACOL_RS09275 ]
- pan locus tag?: SAUPAN004446000
- symbol: SACOL1810
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1810 [new locus tag: SACOL_RS09275 ]
- symbol: SACOL1810
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 1866812..1867765
- length: 954
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236540 NCBI
- RefSeq: YP_186643 NCBI
- BioCyc: see SACOL_RS09275
- MicrobesOnline: 913254 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGGGCAATCATTTCCAATACGCTTTTGAAAACAAACGTTATCACACATGGAATTACCAT
TTAAAAAATAAATTTGGACAAAAAATATTTAAAGTTGCATTGGATGGCGGGTTTGACTGT
CCTAACCGCGATGGCACTGTAGCACATGGTGGATGTACATTTTGTTCTGCTGCAGGTAGC
GGAGACTTTGCAGGTAATCGTGCAGATTCAATCGCAGTACAATTTAAAGAAATTAAAGAA
AAGATGCATGAGAAATGGCACGAAGGAAAATATATTGCTTATTTTCAGGCATTTACAAAT
ACACATGCACCGGTTGAAGTATTAAAAGAAAAATTCGAACCTGTACTTAAAGAACCGGGT
GTTGTGGGATTATCTATTGGTACGCGTCCTGACTGTCTACCAGACGATGTTGTCGAATAT
TTAGCAGATTTGAATCAACGAACATACTTATGGGTTGAATTAGGACTACAAACAATCCAT
CAGTCAACATCTGATTTAATCAATCGTGCCCATGATATGAAAACTTATTATGATGGTGTG
GCAAAATTACGTAAGCATAATATCAATGTATGTACACACATCATTAATGGCTTACCTGGC
GAAGACTATGACATGATGATGGCTACTGCCAAAGAAGTTGCACAAATGGATGTACAAGGT
ATTAAAATTCATTTACTTCATTTGTTAAAAGGTACACCGATGGTAAAACAATACGATAAA
GGTTTATTAACTTTTATGACTCAAGAAGAGTACACAAACCTAGTTGTGGACCAATTAGAA
GTGATTCCCCCTGAAATGATCGTTCACCGAATTACCGGTGATGGTCCAATAGATATCATG
GTAGGTCCAATGTGGAGTGTTAATAAATGGGAAGTATTAAATGGCATCGATGCTGAATTA
GCACGTAGAAATTCTTATCAAGGCTTGCGTTACAAGTCTAAGGTGAAGCAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1810 [new locus tag: SACOL_RS09275 ]
- symbol: SACOL1810
- description: hypothetical protein
- length: 317
- theoretical pI: 7.03345
- theoretical MW: 36053.1
- GRAVY: -0.393691
⊟Function[edit | edit source]
- TIGRFAM: Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 433.5)and 15 moreTranscription DNA-dependent RNA polymerase radical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 63)Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 39.6)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin coproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 38.8)putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 32)radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 28.2)Biosynthesis of cofactors, prosthetic groups, and carriers Biotin biotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 27.3)Hypothetical proteins Conserved radical SAM enzyme, TIGR01210 family (TIGR01210; HMM-score: 24.4)Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 22.9)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin oxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 22.8)Protein synthesis tRNA and rRNA base modification tRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 19.6)Cellular processes Toxin production and resistance radical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 18.3)Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme, archaeal-type (TIGR01578; HMM-score: 15.1)hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 14.7)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 12.3)
- TheSEED :
- [4Fe-4S]-AdoMet protein YtqA
- PFAM: no clan defined Radical_SAM_C; Radical_SAM C-terminal domain (PF16199; HMM-score: 82.9)and 1 moreTIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 52.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.251462
- TAT(Tat/SPI): 0.001225
- LIPO(Sec/SPII): 0.00343
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)