From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01950
  • pan locus tag?: SAUPAN004576000
  • symbol: SAOUHSC_01950
  • pan gene symbol?: epiD
  • synonym:
  • product: flavoprotein EpiD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01950
  • symbol: SAOUHSC_01950
  • product: flavoprotein EpiD
  • replicon: chromosome
  • strand: -
  • coordinates: 1852916..1853434
  • length: 519
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGAGAAAATGTATTGATTTGTTTGTGTGGCTCAGTTAATAGTATCAATATTAGCCAC
    TATATTATTGAATTAAAATCAAAATTTGATGAAGTAAATGTAATCGCGTCAACAAACGGT
    AGAAAGTTTATTAATGGTGAAATATTAAAGCAATTTTGCGATAACTATTATGACGAATTT
    GAAGACCCTTTTTTAAATCATGTAGATATAGCAAATAAACATGACAAGATTATTATTTTA
    CCTGCGACTTCTAATACGATTAATAAAATTGCAAATGGTATATGTGACAATTTATTATTA
    ACTATTTGTCATACAGCTTTTGAAAAACTTTCTATATTTCCAAATATGAATTTACGAATG
    TGGGAAAATCCAGTTACTCAAAATAATATTCGATTATTAAAAGATTATGGTGTATCAATA
    TATCCAGCAAATATTTCAGAAAGTTATGAATTAGCGTCAAAAACATTTAAAAAGAATGTT
    GTCGCACCTGAACCATATAAAGTTCTGGAATTCATTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    519

⊟Protein[edit | edit source]

Protein Data Bank: 3QJG

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01950
  • symbol: SAOUHSC_01950
  • description: flavoprotein EpiD
  • length: 172
  • theoretical pI: 5.63756
  • theoretical MW: 19593.4
  • GRAVY: -0.0581395

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 97.8)
    and 5 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase (TIGR02113; EC 4.1.1.36; HMM-score: 73.2)
    Unknown function Enzymes of unknown specificity polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases (TIGR00421; EC 4.1.1.-; HMM-score: 28.4)
    Metabolism Energy metabolism Electron transport archaeoflavoprotein AfpA (TIGR02699; HMM-score: 26)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 25.9)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, B subunit (TIGR02852; HMM-score: 16)
  • TheSEED  :
    • Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
  • PFAM:
    no clan defined Flavoprotein; Flavoprotein (PF02441; HMM-score: 104.6)
    and 1 more
    His-Me_finger (CL0263) HNH_2; HNH endonuclease (PF13391; HMM-score: 16.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004437
    • TAT(Tat/SPI): 0.000177
    • LIPO(Sec/SPII): 0.000671
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNVVAPEPYKVLEFI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_01950&oldid=98809"