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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1764 [new locus tag: SAUSA300_RS09655 ]
  • pan locus tag?: SAUPAN004576000
  • symbol: epiD
  • pan gene symbol?: epiD
  • synonym:
  • product: lantibiotic epidermin biosynthesis protein EpiD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1764 [new locus tag: SAUSA300_RS09655 ]
  • symbol: epiD
  • product: lantibiotic epidermin biosynthesis protein EpiD
  • replicon: chromosome
  • strand: -
  • coordinates: 1949225..1949743
  • length: 519
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGGAGAAAATGTATTGATTTGTTTGTGTGGCTCAGTTAATAGTATCAATATTAGCCAC
    TATATTATTGAATTAAAATCAAAATTTGATGAAGTAAATGTAATCGCGTCAACAAACGGT
    AGAAAGTTTATTAATGGTGAAATATTAAAGCAATTTTGCGATAACTATTATGACGAATTT
    GAAGACCCTTTTTTAAATCATGTAGATATAGCAAATAAACATGACAAGATTATTATTTTA
    CCTGCGACTTCTAATACGATTAATAAAATTGCAAATGGTATATGTGACAATTTATTATTA
    ACTATTTGTCATACAGCTTTTGAAAAACTTTCTATATTTCCAAATATGAATTTACGAATG
    TGGGAAAATCCAGTTACTCAAAATAATATTCGATTATTAAAAGATTATGGTGTATCAATA
    TATCCAGCAAATATTTCAGAAAGTTATGAATTAGCGTCAAAAACATTTAAAAAGAATGTT
    GTCGCACCTGAACCATATAAAGTTCTGGAATTCATTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    519

⊟Protein[edit | edit source]

Protein Data Bank: 3QJG

⊟General[edit | edit source]

  • locus tag: SAUSA300_1764 [new locus tag: SAUSA300_RS09655 ]
  • symbol: EpiD
  • description: lantibiotic epidermin biosynthesis protein EpiD
  • length: 172
  • theoretical pI: 5.63756
  • theoretical MW: 19593.4
  • GRAVY: -0.0581395

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.36?  ExPASy
    Phosphopantothenoylcysteine decarboxylase N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 97.8)
    and 5 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase (TIGR02113; EC 4.1.1.36; HMM-score: 73.2)
    Unknown function Enzymes of unknown specificity polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases (TIGR00421; EC 4.1.1.-; HMM-score: 28.4)
    Metabolism Energy metabolism Electron transport archaeoflavoprotein AfpA (TIGR02699; HMM-score: 26)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 25.9)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, B subunit (TIGR02852; HMM-score: 16)
  • TheSEED  :
    • Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
  • PFAM:
    no clan defined Flavoprotein; Flavoprotein (PF02441; HMM-score: 104.6)
    and 1 more
    His-Me_finger (CL0263) HNH_2; HNH endonuclease (PF13391; HMM-score: 16.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004437
    • TAT(Tat/SPI): 0.000177
    • LIPO(Sec/SPII): 0.000671
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNVVAPEPYKVLEFI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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